LOCUS       sequence-251139-        1407 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD9-pHuji for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD9-pHuji
FEATURES             Location/Qualifiers
     source          1..1407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             157..864
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     570..589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgccggtca aaggaggcac caagtgcatc aaatacctgc tgttcggatt taacttcatc
       61 ttctggcttg ccgggattgc tgtccttgcc attggactat ggctccgatt cgactctcag
      121 accaagagca tcttcgagca agaaactaat agatctatgg tgagcaaggg cgaggagaat
      181 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      241 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
      301 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      361 cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc
      421 aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc
      481 attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag
      541 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      601 gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga gattaagtat
      661 aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
      721 aagaagcccg tgcagctgcc cggcgcctac atcgtggaca tcaagttgga catcgtgtcc
      781 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      841 ggcggcatgg acgagctgta caagagatct aataataatt ccagcttcta cacaggagtc
      901 tatattctga tcggagccgg cgccctcatg atgctggtgg gcttcctggg ctgctgcggg
      961 gctgtgcagg agtcccagtg catgctggga ctgttcttcg gcttcctctt ggtgatattc
     1021 gccattgaaa tagctgcggc catctgggga tattcccaca aggatgaggt gattaaggaa
     1081 gtccaggagt tttacaagga cacctacaac aagctgaaaa ccaaggatga gccccagcgg
     1141 gaaacgctga aagccatcca ctatgcgttg aactgctgtg gtttggctgg gggcgtggaa
     1201 cagtttatct cagacatctg ccccaagaag gacgtactcg aaaccttcac cgtgaagtcc
     1261 tgtcctgatg ccatcaaaga ggtcttcgac aataaattcc acatcatcgg cgcagtgggc
     1321 atcggcattg ccgtggtcat gatatttggc atgatcttca gtatgatctt gtgctgtgct
     1381 atccgcagga accgcgagat ggtctag
//
