LOCUS       sequence-251143-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CDK4/6 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Yang HW, Cappell SD, Jaimovich A, Liu C, Chung M, Daigh LH, Pack LR,
            Fan Y, Regot S, Covert M, Meyer T
  TITLE     Stress-mediated exit to quiescence restricted by increasing 
            persistence in CDK4/6 activation.
  JOURNAL   Elife. 2020 Apr 7;9. pii: 44571. doi: 10.7554/eLife.44571.
  PUBMED    32255427
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-7"; volume: "9. pii: 44571. doi"; pages: " 
            10.7554/eLife.44571"
COMMENT     Sequence Label: pLenti-mCherry-CDK4KTR
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    558..683
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1180..1413
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1598..1642
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1905..2020
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2098..2309
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2310..3248
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3205..3225
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3267..3974
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3412..3430)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3680..3699
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4361..4910
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4505..4522)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4732..4751
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4913..5512
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4913..4932)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5409..5429
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5536..6124
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5589..5609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6007..6018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6331..6964
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7092..7108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7092..7108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7105..7127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7116..7132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7140..7146,7147..7164,7165..7170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7185..7206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7323..7340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7494..8082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7574..7593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8253..9044,9045..9113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8876..8895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9114..9218)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9266..9400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9303..9322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9357..9376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9568..9690,1..511)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
       61 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      121 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      181 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      241 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      301 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      361 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      421 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      481 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      541 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      601 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      661 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      721 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      781 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      841 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
      901 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
      961 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1021 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1081 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1141 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1201 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1261 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1321 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1381 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1441 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1501 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1561 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1621 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1681 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1741 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1801 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1861 agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg
     1921 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1981 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2041 gcagagatcc agtttatcga tgaggccctt tcgtcttcac tcgaggtgcc cgtcagtggg
     2101 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2161 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2221 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2281 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2341 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2401 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2461 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2521 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2581 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2641 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2701 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2761 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     2821 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2881 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2941 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3001 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3061 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3121 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3181 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3241 catttcaggt gtcgtgagga tccaccatgg tgagcaaggg cgaggaggat aacatggcca
     3301 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     3361 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3421 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     3481 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3541 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3601 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3661 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     3721 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3781 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3841 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3901 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3961 acgagctgta caagagtgct ggtggtagtg ctggtggtaa gcgctcggcc ggtctcaacg
     4021 aagacgagtc gccatctaag aagctgccat cgccgaccgt gtcgtcaagg ctggagcgcc
     4081 tgaccctcca gagatcaaga atcttgagca ctagtgatgg aagtaaacat ctcccaggag
     4141 agtccaaatt tcagcagaaa ctggcagaaa tgacttctac tcgaacacga atgcaaaagc
     4201 agaaaatgaa tgatagcatg gatacctcaa acaaggaaga gaagtaagaa ttcctcgagg
     4261 gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac
     4321 ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc
     4381 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4441 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4501 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4561 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4621 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4681 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4741 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4801 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4861 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgaccga
     4921 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     4981 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5041 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5101 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5161 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5221 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5281 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5341 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5401 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5461 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacccgggcg
     5521 gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5701 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5761 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5821 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5881 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5941 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6001 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6061 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6121 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     6181 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     6241 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6301 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     6361 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     6421 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6481 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6541 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6601 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     6661 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6721 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     6781 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6841 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6901 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6961 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7021 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     7081 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7141 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7201 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7261 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7921 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8701 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8881 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9061 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9241 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     9301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9361 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     9421 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     9481 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     9541 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     9601 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     9661 caccagggcc aggggtcaga tatccactga
//
