LOCUS       sequence-25115-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-KanMX6-Purg1-3FLAG
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(58..245)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     166..190
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(269..340)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 cgagctcgtc gctgcggtcg acggatctat attaccctgt tatccctagc ggatctgccg
       61 gtagaggtgt ggtcaataag agcgacctca tactatacct gagaaagcaa cctgacctac
      121 aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat
      181 ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa
      241 ttcgcttatt tagaagtggc gcgccctatt tatcatcatc gtccttatag tccttatcgt
      301 cgtcatcttt gtaatccttg tcatcgtcgt ccttgtagtc catattaatt aacctttgtt
      361 cagtggcaag catattgaat tagttctaat ttagtaattg aagaaaacgg ataagtaaaa
      421 caacaagaaa aatgacaaac gaatgttcaa tgggtagaaa gaaattcgtt tcacaaaatt
      481 agcctttaaa caaagtgaat tgagtaaggc agctaaagta atgaatgaaa cctttgcaat
      541 tgatgttgaa ccgtatggat ttcctccctt atttatagcc ttccagatac tatcaatgaa
      601 agcgctagct cgcatgatac cagataccac gcggacttgc cgcgggcact tcctcgtcag
      661 cgaaccgaaa acaatacctt gtttacatcg ttgcgccttc ttagacctta cgaagtcgcg
      721 gggaagggtc gtgcacgctg tnntcncgng nggngctncc gggggcaata gccgtngcca
      781 ngtcattgtt tagaagaaaa aaattttttg gtatgtaaca ttggattggt gagccgtagc
//
