LOCUS       sequence-251166-       10433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A,
            C840A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM
  TITLE     Angelman syndrome-associated point mutations in the Zn-binding 
            N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding 
            to the proteasome.
  JOURNAL   J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653.
  PUBMED    30257870
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653."
COMMENT     Sequence Label: PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)
FEATURES             Location/Qualifiers
     source          1..10433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2950..2974
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5594..5618)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5637..6136
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5672..5695)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6069..6088
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6157..6756
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6157..6176)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6653..6673
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6823..7411
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6876..6896)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7294..7305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7486..7719
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7838..7856)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7924..8028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8029..8097,8098..8889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8247..8266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9060..9648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9549..9568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9802..9819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10059..10080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10095..10100,10101..10118,10119..10125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10133..10149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10138..10160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10157..10173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10157..10173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10223..10242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcatggcca cagcttgtaa aagatcagga
     3001 gaacctcagt ctgacgacat tgaagctagc cgaatgaagc gagcagctgc aaagcatcta
     3061 atagaacgct actaccacca gttaactgag ggcgctggaa atgaagccgc cacgaatgag
     3121 ttttgtgctt cctgtccaac ttttcttcgt atggataata atgcagcagc tattaaagcc
     3181 ctcgagcttt ataagattaa tgcaaaactc tgtgatcctc atccctccaa gaaaggagca
     3241 agctcagctt accttgagaa ctcgaaaggt gcccccaaca actcctgctc tgagataaaa
     3301 atgaacaaga aaggcgctag aattgatttt aaagatgtga cttacttaac agaagagaag
     3361 gtatatgaaa ttcttgaatt atgtagagaa agagaggatt attccccttt aatccgtgtt
     3421 attggaagag ttttttctag tgctgaggca ttggtacaga gcttccggaa agttaaacaa
     3481 cacaccaagg aagaactgaa atctcttcaa gcaaaagatg aagacaaaga tgaagatgaa
     3541 aaggaaaaag ctgcatgttc tgctgctgct atggaagaag actcagaagc atcttcctca
     3601 aggataggtg atagctcaca gggagacaac aatttgcaaa aattaggccc tgatgatgtg
     3661 tctgtggata ttgatgccat tagaagggtc tacaccagat tgctctctaa tgaaaaaatt
     3721 gaaactgcct ttctcaatgc acttgtatat ttgtcaccta acgtggaatg tgacttgacg
     3781 tatcacaatg tatactctcg agatcctaat tatctgaatt tgttcattat cgtaatggag
     3841 aatagaaatc tccacagtcc tgaatatctg gaaatggctt tgccattatt ttgcaaagcg
     3901 atgagcaagc taccccttgc agcccaagga aaactgatca gactgtggtc taaatacaat
     3961 gcagaccaga ttcggagaat gatggagaca tttcagcaac ttattactta taaagtcata
     4021 agcaatgaat ttaacagtcg aaatctagtg aatgatgatg atgccattgt tgctgcttcg
     4081 aagtgcttga aaatggttta ctatgcaaat gtagtgggag gggaagtgga cacaaatcac
     4141 aatgaagaag atgatgaaga gcccatccct gagtccagcg agctgacact tcaggaactt
     4201 ttgggagaag aaagaagaaa caagaaaggt cctcgagtgg accccctgga aactgaactt
     4261 ggtgttaaaa ccctggattg tcgaaaacca cttatccctt ttgaagagtt tattaatgaa
     4321 ccactgaatg aggttctaga aatggataaa gattatactt ttttcaaagt agaaacagag
     4381 aacaaattct cttttatgac atgtcccttt atattgaatg ctgtcacaaa gaatttggga
     4441 ttatattatg acaatagaat tcgcatgtac agtgaacgaa gaatcactgt tctctacagc
     4501 ttagttcaag gacagcagtt gaatccatat ttgagactca aagttagacg tgaccatatc
     4561 atagatgatg cacttgtccg gctagagatg atcgctatgg aaaatcctgc agacttgaag
     4621 aagcagttgt atgtggaatt tgaaggagaa caaggagttg atgagggagg tgtttccaaa
     4681 gaattttttc agctggttgt ggaggaaatc ttcaatccag atattggtat gttcacatac
     4741 gatgaatcta caaaattgtt ttggtttaat ccatcttctt ttgaaactga gggtcagttt
     4801 actctgattg gcatagtact gggtctggct atttacaata actgtatact ggatgtacat
     4861 tttcccatgg ttgtctacag gaagctaatg gggaaaaaag gaacttttcg tgacttggga
     4921 gactctcacc cagttctata tcagagttta aaagatttat tggagtatga agggaatgtg
     4981 gaagatgaca tgatgatcac tttccagata tcacagacag atctttttgg taacccaatg
     5041 atgtatgatc taaaggaaaa tggtgataaa attccaatta caaatgaaaa caggaaggaa
     5101 tttgtcaatc tttattctga ctacattctc aataaatcag tagaaaaaca gttcaaggct
     5161 tttcggagag gttttcatat ggtgacaaat gaatctccct taaagtactt attcagacca
     5221 gaagaaattg aattgcttat atgtggaagc cggaatctag atttccaagc actagaagaa
     5281 actacagaat atgacggtgg ctataccagg gactctgttc tgattaggga gttctgggaa
     5341 atcgttcatt catttacaga tgaacagaaa agactcttct tgcagtttac aacgggcaca
     5401 gacagagcac ctgtgggagg actaggaaaa ttaaagatga ttatagccaa aaatggccca
     5461 gacacagaaa ggttacctac atctcatact gcctttaatg tgcttttact tccggaatac
     5521 tcaagcaaag aaaaacttaa agagagattg ttgaaggcca tcacgtatgc caaaggattt
     5581 ggcatgctgt aatacccaac tttcttgtac aaagtggttc gataacgaat tctaccgggt
     5641 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     5701 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     5761 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     5821 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     5881 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     5941 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     6001 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     6061 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     6121 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     6181 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6241 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6301 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6361 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6421 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6481 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6541 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6601 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6661 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6721 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     6781 ccgaccgaaa ggagcgcacg accccatgca tcgatagatc ctaatcaacc tctggattac
     6841 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6901 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6961 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7021 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7081 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7141 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7201 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7261 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7321 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7381 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca
     7441 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7501 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7561 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7621 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7681 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7741 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa
     7801 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     7861 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7921 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7981 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8041 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8101 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8161 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8221 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     8281 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9181 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
     9901 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9961 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
    10021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10081 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10141 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
    10201 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10261 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10321 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10381 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
