LOCUS       sequence-25117-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-hphMX6-Purg1-3FLAG
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(63..250)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     171..195
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(274..345)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 cgcgagctcg tcgctgcagg gtcgacggat ctatattacc ctgtttatcc ctagcggatc
       61 tgccggtaga ggtgtggtca ataagagcga cctcatacta tacctgagaa agcaacctga
      121 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt
      181 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata
      241 agaaattcgc ttatttagaa gtggcgcgcc ctatttatca tcatcgtcct tatagtcctt
      301 atcgtcgtca tctttgtaat ccttgtcatc gtcgtccttg tagtccatat taattaacct
      361 ttgttcagtg gcaagcatat tgaattagtt ctaatttagt aattgaagaa aacggataag
      421 taaaacaaca agaaaaatga caaacgaatg ttcaatgggt agaaagaaat tcgtttcaca
      481 aaattagcct ttaaacaaag tgaattgagt aaggcagcta aagtaatgaa tgaaaccttt
      541 gcaattgatg ttgaaccgta tggatttcct cccttattta tagccttcca gatactatca
      601 atgaaagcgc tagctcgcat gataccagat accacgcgga cttgccgcgg gcacttcctc
      661 gtcagcgaac cgaaaacaat accttgttta catcgttgcg ccttcttaga ccttacgaag
      721 tcgcggggaa gggtcgtgca cgcatgttct cgagcggcgc taccgcgggc aatagccgac
      781 gcagntcttg ntttaaaaaa aaaaaaattt ttttggnatg nanngnatng gaatnggtgg
      841 agg
//
