LOCUS       sequence-251199-        1281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid of targeted ASAP2f knockin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1281)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ22-AAVS1-Puro-CAG-ASAP2f
FEATURES             Location/Qualifiers
     source          1..1281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagacga ctgtgaggta tgaacagggg tcagagctca ctaaaacttc gagctctcca
       61 acagcagatg agcccacgat aaagattgat gatggtcgtg atgagggtaa tgaacaagac
      121 agctgttcca ataccattag gagaaaaatt tccccgtttg tgatgtcatt tggattcaga
      181 gtatttggag ttgtgcttat cattgtagac atcatagtgg tgattgtgga tctggccatc
      241 agtgagaaga aaagaggcat tagagagatt cttgaaggtg tttccctggc tatagcactc
      301 ttcttccttg ttgatgttct catgagagtg tttgttgaag gcttcaagaa ctatttccgg
      361 tccaaactga atactttgga tgcagtcata gtagtgggca ctctgctaat taatatgacc
      421 tactccttct ctgaccttag ctttaacagc cataacgtgt atattaccgc ggataaacag
      481 aaaaacggca ttaaagcgaa ctttaccgtg cgccataacg tggaagatgg cagcgtgcag
      541 ctggcggatc attatcagca gaacaccccg attggcgatg gcccggtgct gctgccggat
      601 aaccattatc tgagcaccca gaccgtgctg agcaaagatc cgaacgaaaa acgcgatcac
      661 atggtgctgc tggaatttgt gaccgcagcg ggcattacac acggcatgga tgaactgtat
      721 ggcggcaccg gcggcagcgc gagccagggc gaagaactgt ttaccggcgt ggtgccgatt
      781 ctggtggaac tggatggcga tgtgaacggc cataaattta gcgtgcgcgg cgaaggcgaa
      841 ggcgatgcga ccattggcaa actgaccctg aaatttattt gcaccaccgg caaactaccg
      901 gtgccgtggc cgaccctggt gaccacctta acctatggcg tgcagtgctt tagccgctat
      961 ccggatcata tgaaacgcca tgattttttt aaaagcgcga tgccggaagg ctatgtgcag
     1021 gaacgcacca ttagctttaa agatgatggc aaatataaaa cccgcgcggt ggtgaaattt
     1081 gaaggcgata ccctggtgaa ccgcattgaa ctgaaaggca ccgattttaa agaagatggc
     1141 aacattctgg ggcataaact ggaatataac acagatcaga tgccgcagat ggttactctt
     1201 cttcgagttc tgagaattgt tatcttaata agaatatttc gcctggcttc acagaagaaa
     1261 caacttgaag tggtaaccta a
//
