LOCUS       sequence-251222-        1245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-induced expression of SOX17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ42-XLone-SOX17
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             949..966
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             955..972
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgagcagcc cggatgcggg atacgccagt gacgaccaga gccagaccca gagcgcgctg
       61 cccgcggtga tggccgggct gggcccctgc ccctgggccg agtcgctgag ccccatcggg
      121 gacatgaagg tgaagggcga ggcgccggcg aacagcggag caccggccgg ggccgcgggc
      181 cgagccaagg gcgagtcccg tatccggcgg ccgatgaacg ctttcatggt gtgggctaag
      241 gacgagcgca agcggctggc gcagcagaat ccagacctgc acaacgccga gttgagcaag
      301 atgctgggca agtcgtggaa ggcgctgacg ctggcggaga agcggccctt cgtggaggag
      361 gcagagcggc tgcgcgtgca gcacatgcag gaccacccca actacaagta ccggccgcgg
      421 cggcgcaagc aggtgaagcg gctgaagcgg gtggagggcg gcttcctgca cggcctggct
      481 gagccgcagg cggccgcgct gggccccgag ggcggccgcg tggccatgga cggcctgggc
      541 ctccagttcc ccgagcaggg cttccccgcc ggcccgccgc tgctgcctcc gcacatgggc
      601 ggccactacc gcgactgcca gagtctgggc gcgcctccgc tcgacggcta cccgttgccc
      661 acgcccgaca cgtccccgct ggacggcgtg gaccccgacc cggctttctt cgccgccccg
      721 atgcccgggg actgcccggc ggccggcacc tacagctacg cgcaggtctc ggactacgct
      781 ggccccccgg agcctcccgc cggtcccatg cacccccgac tcggcccaga gcccgcgggt
      841 ccctcgattc cgggcctcct ggcgccaccc agcgcccttc acgtgtacta cggcgcgatg
      901 ggctcgcccg gggcgggcgg cgggcgcggc ttccagatgc agccgcaaca ccagcaccag
      961 caccagcacc agcaccaccc cccgggcccc ggacagccgt cgccccctcc ggaggcactg
     1021 ccctgccggg acggcacgga ccccagtcag cccgccgagc tcctcgggga ggtggaccgc
     1081 acggaatttg aacagtatct gcacttcgtg tgcaagcctg agatgggcct cccctaccag
     1141 gggcatgact ccggtgtgaa tctccccgac agccacgggg ccatttcctc ggtggtgtcc
     1201 gacgccagct ccgcggtata ttactgcaac tatcctgacg tgtag
//
