LOCUS       sequence-251228-        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of HLA-G fused to B2M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLR03_pXL001-_B2MHLAG
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(383..409)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
ORIGIN
        1 actagtgcca ccatgtctcg ctccgtggcc ttagctgtgc tcgcgctact ctctctttct
       61 ggcctggagg ctatccagcg tactccaaag attcaggttt actcacgtca tccagcagag
      121 aatggaaagt caaatttcct gaattgctat gtgtctgggt ttcatccatc cgacattgaa
      181 gttgacttac tgaagaatgg agagagaatt gaaaaagtgg agcattcaga cttgtctttc
      241 agcaaggact ggtctttcta tctcttgtac tacactgagt tcacccccac tgaaaaagat
      301 gagtatgcct gccgtgtgaa ccatgtgact ttgtcacagc ccaagatagt taagtgggat
      361 cgagacatgg gtggtggtgg tagtggtggt ggtggtagtg gtggtggtgg tagtaagacg
      421 ccaaggatgg tggtcatggc gccccgaacc ctcttcctgc tgctctcggg ggccctgacc
      481 ctgaccgaga cctgggcggg ctcccactcc atgaggtatt tcagcgccgc cgtgtcccgg
      541 cccggccgcg gggagccccg cttcatcgcc atgggctacg tggacgacac gcagttcgtg
      601 cggttcgaca gcgactcggc gtgtccgagg atggagccgc gggcgccgtg ggtggagcag
      661 gaggggccgg agtattggga agaggagaca cggaacacca aggcccacgc acagactgac
      721 agaatgaacc tgcagaccct gcgcggctac tacaaccaga gcgaggccag ttctcacacc
      781 ctccagtgga tgattggctg cgacctgggg tccgacggac gcctcctccg cgggtatgaa
      841 cagtatgcct acgatggcaa ggattacctc gccctgaacg aggacctgcg ctcctggacc
      901 gcagcggaca ctgcggctca gatctccaag cgcaagtgtg aggcggccaa tgtggctgaa
      961 caaaggagag cctacctgga gggcacgtgc gtggagtggc tccacagata cctggagaac
     1021 gggaaggaga tgctgcagcg cgcggacccc cccaagacac acgtgaccca ccaccctgtc
     1081 tttgactatg aggccaccct gaggtgctgg gccctgggct tctaccctgc ggagatcata
     1141 ctgacctggc agcgggatgg ggaggaccag acccaggacg tggagctcgt ggagaccagg
     1201 cctgcagggg atggaacctt ccagaagtgg gcagctgtgg tggtgccttc tggagaggag
     1261 cagagataca cgtgccatgt gcagcatgag gggctgccgg agcccctcat gctgagatgg
     1321 aagcagtctt ccctgcccac catccccatc atgggtatcg ttgctggcct ggttgtcctt
     1381 gcagctgtag tcactggagc tgcggtcgct gctgtgctgt ggagaaagaa gagctcagat
     1441 tgagaattc
//
