LOCUS       sequence-251230-        1476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor template to knock in HLA-E fused to B2M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1476)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLR04_AAVS1_Donor_HLAEB2M
FEATURES             Location/Qualifiers
     source          1..1476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtagatg gaaccctcct tttactcctc tcggaggccc tggcccttac ccagacctgg
       61 gcgggctccc actccttgaa gtatttccac acttccgtgt cccggcccgg ccgcggggag
      121 ccccgcttca tctctgtggg ctacgtggac gacacccagt tcgtgcgctt cgacaacgac
      181 gccgcgagtc cgaggatggt gccgcgggcg ccgtggatgg agcaggaggg gtcagagtat
      241 tgggaccggg agacacggag cgccagggac accgcacaga ttttccgagt gaatctgcgg
      301 acgctgcgcg gctactacaa tcagagcgag gccgggtctc acaccctgca gtggatgcat
      361 ggctgcgagc tggggcccga cgggcgcttc ctccgcgggt atgaacagtt cgcctacgac
      421 ggcaaggatt atctcaccct gaatgaggac ctgcgctcct ggaccgcggt ggacacggcg
      481 gctcagatct ccgagcaaaa gtcaaatgat gcctctgagg cggagcacca gagagcctac
      541 ctggaagaca catgcgtgga gtggctccac aaatacctgg agaaggggaa ggagacgctg
      601 cttcacctgg agcccccaaa gacacacgtg actcaccacc ccatctctga ccatgaggcc
      661 accctgaggt gctgggccct gggcttctac cctgcggaga tcacactgac ctggcagcag
      721 gatggggagg gccataccca ggacacggag ctcgtggaga ccaggcctgc aggggatgga
      781 accttccaga agtgggcagc tgtggtggtg ccttctggag aggagcagag atacacgtgc
      841 catgtgcagc atgaggggct acccgagccc gtcaccctga gatggaagcc ggcttcccag
      901 cccaccatcc ccatcgtggg catcattgct ggcctggttc tccttggatc tgtggtctct
      961 ggagctgtgg ttgctgctgt gatatggagg aagaagagct caggtggaaa aggagggagc
     1021 tactctaagg ctgagtggag cgacagtgcc caggggtctg agtctcacag cttgggagga
     1081 ggtggaagcg gaggaggagg aagcggagga ggaggtagct ctcgctccgt ggccttagct
     1141 gtgctcgcgc tactctctct ttctggcctg gaggctatcc agcgtactcc aaagattcag
     1201 gtttactcac gtcatccagc agagaatgga aagtcaaatt tcctgaattg ctatgtgtct
     1261 gggtttcatc catccgacat tgaagttgac ttactgaaga atggagagag aattgaaaaa
     1321 gtggagcatt cagacttgtc tttcagcaag gactggtctt tctatctctt gtactacact
     1381 gaattcaccc ccactgaaaa agatgagtat gcctgccgtg tgaaccatgt gactttgtca
     1441 cagcccaaga tagttaagtg ggatcgagac atgtaa
//
