LOCUS       sequence-251245-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  minicircle parental backbone plasmid including GFP knock-in donor 
            targeting Tubb3 gene and one cutting site for HITI .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, 
            Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, 
            Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X,
            Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, 
            Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang 
            K, Callaway EM, Zhang K, Belmonte JC
  TITLE     In vivo genome editing via CRISPR/Cas9 mediated homology-independent
            targeted integration.
  JOURNAL   Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub
            2016 Nov 16.
  PUBMED    27851729
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20565"; journalName: "Nature"; date: "2016-12-1- 1"; 
            volume: "540"; issue: "7631"; pages: "144-149"
COMMENT     Sequence Label: pTubb3-MC
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..49
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcgcagctt atagtcgcgc ccggngagcc caagggcacg ccctggcacc gcggccgcnn
       61 nnnctcgacc atcatcatca tcatcattga gtttatctgg gataacaggg taatgtcatc
      121 tagggataac agggtatgtc atctgggata acagggtaat gtatctaggg ataacagggt
      181 aatgtcatct gggataacag ggtaatgtca tctagggata acagggtatg tcatctggga
      241 taacagggta atgtatctag ggataacagg gtaatgtcat ctgggataac agggtaatgt
      301 catctaggga taacagggta tgtcatctgg gataacaggg taatgtatct agggataaca
      361 gggtaatgtc atctgggata acagggtaat gtcatctagg gataacaggg tatgtcatct
      421 gggataacag ggtaatgtat ctagggataa cagggtaatg tcatctggga taacagggta
      481 atgtcatcta gggataacag ggtatgtcat ctgggataac agggtaatgt atctagggat
      541 aacagggtaa tgtcatctgg gataacaggg taatgtcatc tagggataac agggtatgtc
      601 atctgggata acagggtaat gtatctaggg ataacagggt aatgtcatct gggataacag
      661 ggtaatgtca tctagggata acagggtatg tcatctggga taacagggta atgtatctag
      721 ggataacagg gtaatgtcat ctgggataac agggtaatgt catctaggga taacagggta
      781 tgtcatctgg gataacaggg taatgtatct agggataaca gggtaatgtc aaacggtctc
      841 cagcttggct gnttttgnng gntgagagaa gaatcgccag cgtcgcacag catgtttgct
      901 tgtcgccgtc gcgnnntgtc annatctttt ccnccagcag ttagggantn agcgtcttaa
      961 nnctggcgcg a
//
