LOCUS       sequence-25125-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ150 - pDESTttTi5605[R4-R3]
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..269)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(277..294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(277..293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca actttattat acatagttga taattcactg gccgtcgttt tacactcgag
      301 acgtacggtg cgcgcgatgc attcgaagat ctgcccacta gtgagtcgta ttataagtgc
      361 aagtaagatc agtgtttgtt tcgatatcag tctgtttcgt aacggtcttc tgtataacta
      421 caaaaaagaa taaaaaaccg tatcacaact gagaaaaaag acaaaactta ttgaacagtg
      481 ttcctttcaa aaaattattt tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat
      541 caaaaacaag aaaactaacg tgttctcctg ggcgtggaac tccttatcaa gattaccatt
      601 attgtctgtc agaattgata tttcaattat ttcatatcta gacaaacccg ggtgtcttgc
      661 atttattagt actgtgagtt tac
//
