LOCUS       sequence-25126-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ150 - pDESTttTi5605[R4-R3]
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(102..107,108..125,126..132)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(252..271)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
       61 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
      121 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
      181 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      241 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      301 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      361 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
      421 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      481 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      541 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
      601 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
      661 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
      721 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
      781 tccatgt
//
