LOCUS       sequence-25127-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ151 - ttTi5605_MCS
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..43)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        111..215
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(434..453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtnccgaaac gcgcgagnga aagggcctcg tgatacgcct atttttatag gttaatgtca
       61 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      181 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      241 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      301 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      361 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      421 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      481 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      541 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      601 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      661 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaccgg agctgaatga
      721 ngcntaccaa cgacgagcgt gacacacgat gctgagcatg ncacacgtgc ncnganataa
      781 ctgcgacnct accnactccc gcacatataa cggatgagcg a
//
