LOCUS       sequence-251295-        3183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  apobb.1 homology arm 5' gateway clone .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Thierer JH, Ekker SC, Farber SA
  TITLE     The LipoGlo reporter system for sensitive and specific monitoring of
            atherogenic lipoproteins.
  JOURNAL   Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w.
  PUBMED    31366908
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-31"; volume: "10(1):3426. doi"; pages: " 
            10.1038/s41467-019-11259-w"
COMMENT     Sequence Label: pDONR_P4-P1R_5'arm_apobb.1
FEATURES             Location/Qualifiers
     source          1..3183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(133..160)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(252..338)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     387..409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     401..418
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     402..418
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    458..553
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1093..1217
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1289..1308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1290..1308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1313..1329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1313..1329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1431..1450)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1442..2251
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1509..1528)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2398..2986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2887..2906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3140..3157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
      121 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      181 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      241 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      301 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      361 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
      421 taagctcggg cccgagttaa cgctaccatg gagctccaaa taatgatttt attttgactg
      481 atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat gccaactttg
      541 tatagaaaag ttgcgctgcc tggaatgaat gaagctacac tggctgaaat ttgcaagaaa
      601 atccaattag ttgttgtaga aatgctgagg aattttggta acaacttgga ggtatacttc
      661 tctcccatca tggataactt taatacaatt caattgacat ttcccaatgg aaaggtcatg
      721 acagtagctg agttccaaca aaatgtgcag agtatcctga gaagcaacct agttatgata
      781 gcagatgcaa tgaagcaaat cgaaagtcct gatgtagttc ttaagaaact tggacaaact
      841 ttacaagagg ttgttgaaaa aggacaagag tttgtggaca aaatgaattt aagtttatta
      901 gaagatattg ccgcggccat taacacgttc tacctggagc tcatgaagat catagaagac
      961 attagtgagg cagtcatgtt tggtttttca atccctgcaa ttaaaatata tgaaatgtct
     1021 caaaacatag agaaagtgtt gaacgcaaat aatggaatac atcaatttga gcttcctctc
     1081 ccatttttcc agacaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat
     1141 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     1201 catatgcagt cactatgaac caactactta gatggtatta gtgacctgta gaattcgagc
     1261 tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc
     1321 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1381 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1441 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1501 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1561 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1621 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1681 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1741 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1801 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1861 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1921 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1981 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2041 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2101 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2161 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2221 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2281 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2341 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2401 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2461 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2521 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2581 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2641 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2701 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2761 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2821 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2881 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2941 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3001 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3061 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3181 caa
//
