LOCUS       sequence-251304-        3242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - negative control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSM105
FEATURES             Location/Qualifiers
     source          1..3242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    108..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             321..1034
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1114..1134
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(1362..2153,2154..2222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1985..2004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2223..2327)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2371..2402)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(2546..2563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2680..3225)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2760..2779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaaaaaa aaaccccgcc cctgacaggg cggggttttt tttccaatta ttgaaggccg
       61 ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa tgactaagaa ttcgcggccg
      121 cttctagagc gtctcacttc ggatccgctg caggcttccg cacccatagc atttttatcc
      181 ataagattag cggatcctac cttttacttc ttgccatcga ctacggatac tgagcacgac
      241 atgcatgcca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta
      301 atcatgctaa ggagacttca atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
      361 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
      421 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
      481 cggtaccttg gccgactctg gtaacgacgc tgacttgggg tgttcagtgc tttgctcgtt
      541 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
      601 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
      661 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg
      721 gcaatatcct gggccataag ctggaataca attacatcag cgacaatgtt tacatcaccg
      781 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
      841 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
      901 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
      961 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     1021 atgaactgta caaataatgg cctcggtacc aaagacgaac aataagacgc tgaaaagcgt
     1081 cttttttcgt tttggtccgc tgcgtttgag acgtactagt agcggccgct gcagttaatc
     1141 actgattaac tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc
     1201 gttttggtcc ttttgttatc aataaaaaag gggagcggtt tcccgctccc cttattgttc
     1261 gtctacagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1501 cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2341 aaagtgccac ctggagtcct gttgagtaat agtcaaaagc ctccggtcgg aggcttttga
     2401 ctttctgctt acccaatagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     2461 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     2521 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     2581 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     2641 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     2701 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     2761 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     2821 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     2881 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     2941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     3001 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     3061 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     3121 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     3181 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     3241 aa
//
