LOCUS       sequence-251332-        3413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSPB848
FEATURES             Location/Qualifiers
     source          1..3413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    108..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1285..1305
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(1533..2324,2325..2393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2156..2175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2394..2498)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2542..2573)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(2717..2734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2851..3396)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2931..2950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaaaaaa aaaccccgcc cctgacaggg cggggttttt tttccaatta ttgaaggccg
       61 ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa tgactaagaa ttcgcggccg
      121 cttctagagc gtctcacttc ggatccgctg caggcttccg cacccatagc atttttatcc
      181 ataagattag cggatcctac cttttacttc ttgccatcga ctacggatac tgagcacgac
      241 atgcatgcca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta
      301 atcatgctaa ggagacttca atgtccgaag ggcgtcgtgg tctgggtcga gggctttcgg
      361 ccctgctggg cgaggtcgac gccgcgccgg ctcaggcgcc gggcgagcag cttggcggtt
      421 cccgtgaagc gccgatcgaa atcctgcagc gcaatcccga ccagccccgt cggaccttcc
      481 gcgaggaaga cctcgaggat ctgtcaaact cgatccgtga gaagggcgtt ctgcagccga
      541 tcctggtgcg gccgtcgccg gatacggctg gcgagtacca gatcgtcgcc ggcgagcgcc
      601 gttggcgcgc agcccagcgg gcgggcctca agactgtgcc gatcatggtg cgcgagctcg
      661 acgacctggc ggtgctggag atcggcatca tcgagaacgt ccagcgggct gatctgaacg
      721 tcctggaaga agcgctctcc tacaaggtgc tgatggagaa gttcgagcgc acccaggaga
      781 acatcgccca gaccatcggc aagagccgca gccacgtggc caacacgatg cgcttgctgg
      841 ccctaccgga cgaggttcag tcgcacctgg tcagtggtga gctgacggcc ggccacgccc
      901 gcgccatcgc tgcagcggct gacccggtgg ccctcgccaa gcaaatcatc gagggcggct
      961 tgtcggtgcg cgagacagag gccctggccc gtaaggcgcc caacctgtct gcgggcaaga
     1021 gcaagggggg ccgcccccca cgcgtgaagg acaccgacac ccaggcgctg gagtctgacc
     1081 tgtcctcggt tctgggtctg gatgtctcga tcgaccatcg cgggtctacc ggcacgctga
     1141 ccatcaccta cgccaccctc gagcagctcg acgacctctg caaccgactg acgcgcggga
     1201 tctaataatg gcctcggtac caaagacgaa caataagacg ctgaaaagcg tcttttttcg
     1261 ttttggtccg ctgcgtttga gacgtactag tagcggccgc tgcagttaat cactgattaa
     1321 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
     1381 cttttgttat caataaaaaa ggggagcggt ttcccgctcc ccttattgtt cgtctacaga
     1441 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
     1681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2521 cctggagtcc tgttgagtaa tagtcaaaag cctccggtcg gaggcttttg actttctgct
     2581 tacccaatag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     2641 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     2701 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     2761 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     2821 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     2881 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     2941 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     3001 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     3061 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     3121 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     3181 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     3241 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     3301 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     3361 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taa
//
