LOCUS       sequence-251342-        3398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  apobb.1 homology arm 3' gateway clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Thierer JH, Ekker SC, Farber SA
  TITLE     The LipoGlo reporter system for sensitive and specific monitoring of
            atherogenic lipoproteins.
  JOURNAL   Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w.
  PUBMED    31366908
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-31"; volume: "10(1):3426. doi"; pages: " 
            10.1038/s41467-019-11259-w"
COMMENT     Sequence Label: pDONR_P2R-P3_3'arm_apobb.1
FEATURES             Location/Qualifiers
     source          1..3398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..92
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     76..92
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        97..115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    153..248
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1008..1131
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1202..1219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1202..1218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1211..1233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1282..1368
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1460..1487
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1646..1663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1817..2405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1897..1916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2552..3361)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3275..3294
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3353..3372
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
       61 acgggccaga gctgccagga aacagctatg accatgtaat acgactcact ataggggata
      121 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      241 taaagttggg taaggcagac atcagtttgt aagcaaatcc aaaatatttt taatgaagat
      301 taaatgattt tcagtttata tgtcaaaaag gagtagttaa catgtgttaa cctgaagctg
      361 gttaaaataa ggttcaataa ccactaatca aagcaattga tttactagat ttttcaggta
      421 tgggattgag taaaatgtta gtagtttttt ttacctctac aaaagttctg ataaactttt
      481 ttcttcctaa tgaaaaaaat agaataaaat attttatttg taattttttt tttaaagtca
      541 ccagtagttt gcataaaaaa ctaaaatgta cccatgtacc actgcttatt ttacattttg
      601 ttgcatgtta ttacacctgt atattcaatt atattatatt attgaatggt ccaaactagt
      661 gtaaaacact tctagaatat agaaatgaca agattgggtc gtgttgcata ataaaagttc
      721 tttattgatc cccatccatc atgccattaa tgttggatac agaacaagga tgacaatagg
      781 ttgcaggtgg catttttgat aactagctta gtatttcaat cctcatttat acaaatagaa
      841 taatagagca gcataacaat gagacatgtt ttgagtcagt aattgcttta tatacatgtt
      901 tgtactatac ttcagtgaag tgtccattgg cttcgatgtc caagaatgtt catttcaaaa
      961 tatggtagtg tttacttagt ttactttctg ttcagaatga ccatattcca ctttgtacaa
     1021 gaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1081 taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat gaaccaacta
     1141 cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc ccgagcttaa
     1201 gactggccgt cgttttacaa cgtcgtgact gggaaaacat ccatgctagc gttaacgcga
     1261 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt
     1321 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     1381 gatttgaacg ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     1441 gccaggcatc aaactaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1501 actcttcctg gctagcggta cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt
     1561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac
     2461 tcacgttaag ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct
     2521 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     2581 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     2641 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     2701 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2761 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     2821 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     2881 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     2941 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3001 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgtttttc
     3061 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     3121 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     3181 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     3241 agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     3301 aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca
     3361 taacacccct tgtattactg tttatgtaag cagacagt
//
