LOCUS       sequence-251345-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BirA* expressed as a protein fusion with MS2 coat protein (MCP) 
            dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify 
            the RNA proximal proteome by their biotinylation. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, 
            Macek B, Jansen RP
  TITLE     beta-Actin mRNA interactome mapping by proximity biotinylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12863-12872. doi: 
            10.1073/pnas.1820737116. Epub 2019 Jun 12.
  PUBMED    31189591
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1820737116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-06-25- 25"; volume: "116"; issue: "26"; pages: 
            "12863-12872"
COMMENT     Sequence Label: phage - ubc - nls - 2xmcp - egfp - BirA*
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(4..351,352..354,355..963)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 gacaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
       61 tctggcgagc agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc
      121 cagacactga gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg
      181 cctgagccta tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc
      241 gtggccgtgc tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag
      301 ctgaagtccg gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga
      361 cggaagtggt tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag
      421 cagggacctg ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg
      481 ctgagaaagc tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag
      541 gaccgcaagc tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag
      601 atcgtgatcg gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac
      661 cagggctgga tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc
      721 atgctgatca gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct
      781 tacctgagca gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc
      841 ggcgacaagg aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg
      901 gagcaggacg gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag
      961 aagtaa
//
