LOCUS       sequence-251348-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BirA* expressed as a protein fusion with MS2 coat protein (MCP) 
            dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify 
            the RNA proximal proteome by their biotinylation. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     Jansen lab plasmids
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: phage - ubc - nls - 2xmcp - egfp - cMycBirA*
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..30
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(34..381,382..384,385..993)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 gaacaaaaac tcatctcaga agaggatctc gacaaggaca acaccgtgcc cctgaagctg
       61 atcgccctgc tggccaacgg cgagttccac tctggcgagc agctgggaga gaccctggga
      121 atgagcagag ccgccatcaa caagcacatc cagacactga gagactgggg agtggacgtg
      181 ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta tccagctgct gaacgccaag
      241 cagatcctgg gacagctgga tggcggaagc gtggccgtgc tgcctgtgat cgactccacc
      301 aatcagtacc tgctggacag aatcggagag ctgaagtccg gcgacgcctg catcgccgag
      361 taccagcagg ctggcagagg aggcagagga cggaagtggt tcagcccatt cggagccaac
      421 ctgtacctgt ccatgttctg gagactggag cagggacctg ctgctgccat cggactgagt
      481 ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc tgggagccga caaggtgaga
      541 gtgaagtggc ctaatgacct gtacctccag gaccgcaagc tggctggcat cctggtggag
      601 ctgacaggca agacaggcga tgccgctcag atcgtgatcg gagccggaat caacatggcc
      661 atgagaagag tggaggagag cgtggtgaac cagggctgga tcaccctgca ggaggctggc
      721 atcaacctgg accggaacac cctggccgcc atgctgatca gagagctgag agccgctctg
      781 gagctgttcg agcaggaggg actggctcct tacctgagca gatgggagaa gctggacaac
      841 ttcatcaaca gacctgtgaa gctgatcatc ggcgacaagg aaatcttcgg catctccaga
      901 ggaatcgaca agcagggagc tctgctgctg gagcaggacg gaatcatcaa gccctggatg
      961 ggcggagaaa tctccctgag aagcgcagag aagtaa
//
