LOCUS       sequence-251466-         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AtCIB (Arabidopsis thaliana) preceded by restriction 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: pOVC1_X_AtCIB
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..549
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="NGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSSM
                     ITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCNE
                     AAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKEL
                     EKTDYI"
ORIGIN
        1 accatggcca ccggtgaatt ctcaggctct ggatcccccg gtaatggagc tataggaggt
       61 gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc tcacctcaag
      121 tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc ttcaatgatt
      181 accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc gatgatgtac
      241 ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac gacgcttggg
      301 actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa tgaggcggcg
      361 aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga gaagtcgaaa
      421 ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca caaagccaag
      481 aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt ggagaaaacg
      541 gattatattc atcccgggta a
//
