LOCUS       sequence-251469-        9156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express RASA2 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Arafeh R, Qutob N, Emmanuel R, Keren-Paz A, Madore J, Elkahloun A, 
            Wilmott JS, Gartner JJ, Di Pizio A, Winograd-Katz S, Sindiri S, 
            Rotkopf R, Dutton-Regester K, Johansson P, Pritchard AL, Waddell N, 
            Hill VK, Lin JC, Hevroni Y, Rosenberg SA, Khan J, Ben-Dor S, Niv MY,
            Ulitsky I, Mann GJ, Scolyer RA, Hayward NK, Samuels Y
  TITLE     Recurrent inactivating RASA2 mutations in melanoma.
  JOURNAL   Nat Genet. 2015 Dec;47(12):1408-10. doi: 10.1038/ng.3427. Epub 2015 
            Oct 26.
  PUBMED    26502337
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3427"; 
            journalName: "Nat Genet"; date: "2015-12"; volume: "47"; issue: 
            "12"; pages: "1408-10"
COMMENT     Sequence Label: pCDF1-WT_RASA2-EF1-Puro
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..74)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     174..193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        338..541
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1684..1887
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1884..1908
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1958..1967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1967..1990
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4579..4790
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4803..5071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5098..5697
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5098..5117)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5594..5614
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5704..6292
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5757..5777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6175..6186)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6629..6750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6666..6685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6720..6739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6790..6925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6958..6974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6958..6974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6971..6993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6982..6998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7006..7012,7013..7030,7031..7036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7051..7072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7189..7206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7360..7948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7440..7459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8119..8910,8911..8979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8742..8761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8980..9084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(9152..9156,1..14)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
       61 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      181 agagcagatt gtactgagag tgcaccatat gccaagtacg ccccctattg acgtcaatga
      241 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      301 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      361 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      421 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      481 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      541 ttgtgaaact tcgaggagtc tctttgttga ggacttttga gttctccctt gaggctccca
      601 cagatacaat aaatatttga gattgaaccc tgtcgagtat ctgtgtaatc ttttttacct
      661 gtgaggtctc ggaatccggg ccgagaactt cgcagttggc gcccgaacag ggacttgatt
      721 gagagtgatt gaggaagtga agctagagca atagaaagct gttaagcaga actcctgctg
      781 acctaaatag ggaagcagta gcagacgctg ctaacagtga gtatctctag tgaagcagac
      841 tcgagctcat aatcaagtca ttgtttaaag gcccagataa attacatctg gtgactcttc
      901 gcggaccttc aagccaggag attcgccgag ggacagtcaa caaggtagga gagattctac
      961 agcaacatgg ggaatggaca ggggcgagat tggaaaatgg ccattaagag atgtagtaat
     1021 gttgctgtag gagtaggggg gaagagtaaa aaatttggag aagggaattt cagatgggcc
     1081 attagaatgg ctaatgtatc tacaggacga gaacctggtg atataccaga gactttagat
     1141 caactaaggt tggttatttg cgatttacaa gaaagaagag aaaaatttgg atctagcaaa
     1201 gaaattgata tggcaattcc tgcattgagg agaaatggta ggcaatgtgg catgtctgaa
     1261 aaagaggagg aatgatgaag tatctcagac ttattttata agggagatac tgtgctgagt
     1321 tcttcccttt gaggaaggta tgtcatatcc tagacatagt ctcaatttta aaagaagagg
     1381 taggatagga gggatggccc cttatgaatt attagcacaa caagaatcct taagaataca
     1441 agattatttt tctgcaatac cacaaaaatt gcaagcacag tggatttatt ataaagatca
     1501 aaaagataag aaatggaaag gaccaatgag agtagaatac tggggacagg gatcagtatt
     1561 attaaaggat gaagagaagg gatattttct tataatcgat actagtatta tgcccagtac
     1621 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1681 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1741 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1801 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1861 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     1921 catccacgct gttttgacct ccatagaaga ttctagagcc accatggatt acaaggatga
     1981 cgacgataag gcggcggcgg cgcctgctgc tgcggcggct tcttccgagg cgccagcggc
     2041 gagtgcgact gcagagcccg aggccgggga ccaggacagt cgcgaggttc gagtgttgca
     2101 gagcctgcgg ggcaagatct gtgaagcaaa aaatttattg ccatatcttg gaccccacaa
     2161 aatgagagat tgtttctgta ccataaattt ggaccaggaa gaagtttatc gtacccaagt
     2221 tgtggaaaaa tctttaagcc catttttcag tgaagaattt tactttgaga ttccaagaac
     2281 tttccagtat ttgtctttct atgtttatga taagaatgtt ttacaaagag atctccgtat
     2341 aggaaaagta gccatcaaaa aagaagactt gtgtaatcac agtggcaaag aaacttggtt
     2401 ttcattacag cctgttgact ccaattcaga ggttcagggt aaagttcacc ttgaattaaa
     2461 actgaatgaa ctgataacgg agaatggaac tgtatgccag cagcttgttg tacacatcaa
     2521 ggcatgccat gggttgcctc tcataaatgg ccaaagctgt gacccttatg caacagtttc
     2581 tctagtgggc ccttctagga atgaccaaaa gaagacaaaa gtaaagaaga aaacaagcaa
     2641 tccgcagttt aatgaaatct tttattttga ggtaaccaga tccagtagtt acaccagaaa
     2701 gtcccagttc caggtagaag aggaggacat tgaaaagcta gaaatcagga tcgacttgtg
     2761 gaacaatgga aacctagtcc aagatgtttt cctaggtgag attaaggttc ctgtgaacgt
     2821 attaagaact gattcctctc atcaagcctg gtacttgcta cagccaagag acaatggaaa
     2881 caagtcatcc aaaactgatg acctggggtc tcttcgatta aatatatgtt atacagaaga
     2941 ctacgtgctt ccttcagagt actatggtcc tttgaaaact ttgctgctaa aatcaccaga
     3001 tgttcaacca atatctgcct cagctgctta cattttgagt gaaatatgtc gagataaaaa
     3061 tgatgctgtt ttgccccttg tacgactgct gctgcaccat gataaacttg ttccttttgc
     3121 cactgctgtg gctgaattag acttgaagga tacacaagat gcaaacacaa tttttagagg
     3181 aaattccctg gctacccgat gtctggatga gatgatgaaa atagtgggag ggcactacct
     3241 gaaagtaaca ttaaaaccta ttcttgatga gatatgtgac tcctcaaaat cctgtgaaat
     3301 cgatcctatt aaattgaaag agggagataa tgtagaaaat aataaggaga atctgcgcta
     3361 ctatgtagac aagttattca atacaattgt aaaatcaagt atgagctgcc ccactgtaat
     3421 gtgtgatatc ttttattctc taaggcagat ggctactcag agatttccta atgaccctca
     3481 tgttcagtat tctgcagtga gcagctttgt atttcttcgt ttctttgctg tagccgtagt
     3541 atcacctcat acttttcatt tgcgacctca tcatccagat gcacagacaa ttagaacatt
     3601 aactctcatc tcaaaaacta tacaaacttt gggaagctgg gggagtctgt ccaaaagcaa
     3661 gtcaagtttc aaagagacat tcatgtgtga atttttcaaa atgtttcaag aagaaggata
     3721 tattatagca gttaaaaagt tcttggatga aatttcatct actgaaacta aagagtccag
     3781 tggtacgagt gagcctgtgc acctgaaaga aggtgagatg tataaaagag ctcaaggaag
     3841 aactcggatt ggaaaaaaga attttaagaa acgatggttc tgcttaacaa gcagagagct
     3901 cacctaccac aaacagccag gcaaagatgc aatctacaca atcccagtaa aaaacattct
     3961 tgctgtggaa aaactggaag agagctcttt caacaagaaa aatatgttcc aagtaataca
     4021 tacggagaaa ccactctatg tccaggcaaa taactgtgta gaagctaatg aatggataga
     4081 cgtactctgc agggtgagcc gatgcaatca aaacaggctc agtttttatc atccctctgt
     4141 gtatctgaac ggaaattggc tctgctgtca ggagactggt gaaaacactc tcggctgcaa
     4201 gccatgtact gcaggtgtcc ctgcagacat ccaaatagat attgatgaag acagagaaac
     4261 agaaagaatt tattcccttt ttaccctcag tttacttaag ctgcagaaga tggaagaggc
     4321 ttgtggaact attgcagtct atcaaggacc acagaaagag cctgatgatt attctaactt
     4381 tgtaatcgag gattctgtaa caacctttaa gacaattcag caaataaaaa gcataattga
     4441 gaagctggat gaacctcatg aaaaatatag gaagaaaaga tccagtagtg caaaatatgg
     4501 gagcaaggaa aatccaattg ttgggaaagc atcttaggcg gccgcgaagg atctgcgatc
     4561 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4621 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4681 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4741 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     4801 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     4861 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     4921 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     4981 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     5041 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagatg
     5101 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5161 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5221 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5281 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5341 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5401 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5461 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5521 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5581 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5641 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc
     5701 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5761 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5821 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5881 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5941 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6001 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6061 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6121 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6181 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6241 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     6301 gatgacagag ttagaagatc gcttcaggaa gctatttggc acgacttcta caacgggaga
     6361 cagcacagta gattctgaag atgaacctcc taaaaaagaa aaaagggtgg actgggatga
     6421 gtattggaac cctgaaatcg atagcttcca gtgctttgtg aaacttcgag gagtctcttt
     6481 gttgaggact tttgagttct cccttgaggc tcccacagat acaataaata tttgagattg
     6541 aaccctgtcg agtatctgtg taatcttttt tacctgtgag gtctcggaat ccgggccgag
     6601 aacttcgcag cgagctcatt gtaccgcgaa cttgtttatt gcagcttata atggttacaa
     6661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6721 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6781 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6901 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     6961 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7021 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7081 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7141 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8221 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8701 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9001 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9061 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9121 aaccattatt atcatgacat taacctataa aaatag
//
