LOCUS       sequence-251493-         384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EcSSPB-nano (Escherichia coli) preceded by restriction 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: pOVC1_X_EcSSPB-micro
FEATURES             Location/Qualifiers
     source          1..384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(43..249,250..252,253..372)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 accatggcca ccggtgaatt ctcaggctct ggatcccccg ggagctcccc gaaacgccct
       61 aagctgctgc gtgaatatta cgattggctg gttgataaca gctttacccc atatctggtg
      121 gtggatgcca catacctggg cgtgaacgtg cccgtggagt atgtgaaaga cggtcagatc
      181 gtgctgaatc tgtctgcaag tgcgaccggc aacctgcaac tgacaaatga ttttatccag
      241 ttcaacgccc agtttaaggg cgtgtctcgt gaactgtata tcccgatggg tgccgctctg
      301 gccatttacg ctcgcgagaa cggcgatggt gtgatgttcg aaccagaaga aatctatgac
      361 gagctgaata ttggtcccgg gtaa
//
