LOCUS       sequence-251497-        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: Opto-casp9_VfAU1-LOV_casp9
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(811..822)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accatggcca ccggtcctga ctacagtctc gtgaaggctc tgcaaatggc acaacagaat
       61 tttgtcatta cagacgcctc cctcccagac aaccctatcg tctacgccag tagagggttt
      121 ctgacactga caggctattc tctcgaccag atcctgggca ggaactgcag gtttctgcaa
      181 gggccagaaa cagacccaag agctgtggat aagatcagga atgccatcac caaaggcgtt
      241 gataccagtg tctgtctgct gaattataga caggatggca caaccttctg gaatctcttc
      301 ttcgtggctg gactcagaga ttctaagggc aatattgtca actacgtcgg agtgcagtca
      361 aaggtgagcg aagattatgc caagctgctg gtcaacgagc agaacattga gtacaaaggt
      421 gtgcgcacca gtaacatgct gcgcagaaag cccggtgaat tctcaggctc tggatccccc
      481 gggggatttg gtgatgtcgg tgctcttgag agtttgaggg gaaatgcaga tttggcttac
      541 atcctgagca tggagccctg tggccactgc ctcattatca acaatgtgaa cttctgccgt
      601 gagtccgggc tccgcacccg cactggctcc aacatcgact gtgagaagtt gcggcgtcgc
      661 ttctcctcgc tgcatttcat ggtggaggtg aagggcgacc tgactgccaa gaaaatggtg
      721 ctggctttgc tggagctggc gcggcaggac cacggtgctc tggactgctg cgtggtggtc
      781 attctctctc acggctgtca ggccagccac ctgcagttcc caggggctgt ctacggcaca
      841 gatggatgcc ctgtgtcggt cgagaagatt gtgaacatct tcaatgggac cagctgcccc
      901 agcctgggag ggaagcccaa gctctttttc atccaggcct gtggtgggga gcagaaagac
      961 catgggtttg aggtggcctc cacttcccct gaagacgagt cccctggcag taaccccgag
     1021 ccagatgcca ccccgttcca ggaaggtttg aggaccttcg accagctgga cgccatatct
     1081 agtttgccca cacccagtga catctttgtg tcctactcta ctttcccagg ttttgtttcc
     1141 tggagggacc ccaagagtgg ctcctggtac gttgagaccc tggacgacat ctttgagcag
     1201 tgggctcact ctgaagacct gcagtccctc ctgcttaggg tcgctaatgc tgtttcggtg
     1261 aaagggattt ataaacagat gcctggttgc tttaatttcc tccggaaaaa acttttcttt
     1321 aaaacatcac ccgggtaa
//
