LOCUS       sequence-251505-        1443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of 
            Opto-casp9) separated by P2A site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: VfAU1-LOV_P2A_casp9
FEATURES             Location/Qualifiers
     source          1..1443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             463..489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             490..546
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             547..576
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(916..927)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accatggcca ccggtcctga ctacagtctc gtgaaggctc tgcaaatggc acaacagaat
       61 tttgtcatta cagacgcctc cctcccagac aaccctatcg tctacgccag tagagggttt
      121 ctgacactga caggctattc tctcgaccag atcctgggca ggaactgcag gtttctgcaa
      181 gggccagaaa cagacccaag agctgtggat aagatcagga atgccatcac caaaggcgtt
      241 gataccagtg tctgtctgct gaattataga caggatggca caaccttctg gaatctcttc
      301 ttcgtggctg gactcagaga ttctaagggc aatattgtca actacgtcgg agtgcagtca
      361 aaggtgagcg aagattatgc caagctgctg gtcaacgagc agaacattga gtacaaaggt
      421 gtgcgcacca gtaacatgct gcgcagaaag cccggtgaat tctacccata cgatgttcca
      481 gattacgctg cgaccaactt tagcctgctg aaacaggcgg gcgatgtgga agaaaacccg
      541 ggcccggaac aaaaactcat ctcagaagag gatctgggat cccccggggg atttggtgat
      601 gtcggtgctc ttgagagttt gaggggaaat gcagatttgg cttacatcct gagcatggag
      661 ccctgtggcc actgcctcat tatcaacaat gtgaacttct gccgtgagtc cgggctccgc
      721 acccgcactg gctccaacat cgactgtgag aagttgcggc gtcgcttctc ctcgctgcat
      781 ttcatggtgg aggtgaaggg cgacctgact gccaagaaaa tggtgctggc tttgctggag
      841 ctggcgcggc aggaccacgg tgctctggac tgctgcgtgg tggtcattct ctctcacggc
      901 tgtcaggcca gccacctgca gttcccaggg gctgtctacg gcacagatgg atgccctgtg
      961 tcggtcgaga agattgtgaa catcttcaat gggaccagct gccccagcct gggagggaag
     1021 cccaagctct ttttcatcca ggcctgtggt ggggagcaga aagaccatgg gtttgaggtg
     1081 gcctccactt cccctgaaga cgagtcccct ggcagtaacc ccgagccaga tgccaccccg
     1141 ttccaggaag gtttgaggac cttcgaccag ctggacgcca tatctagttt gcccacaccc
     1201 agtgacatct ttgtgtccta ctctactttc ccaggttttg tttcctggag ggaccccaag
     1261 agtggctcct ggtacgttga gaccctggac gacatctttg agcagtgggc tcactctgaa
     1321 gacctgcagt ccctcctgct tagggtcgct aatgctgttt cggtgaaagg gatttataaa
     1381 cagatgcctg gttgctttaa tttcctccgg aaaaaacttt tctttaaaac atcacccggg
     1441 taa
//
