LOCUS       sequence-251560-        7811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses nut lipid binding TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7811)
  TITLE     Alcocer Lab plasmids
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMJA290
FEATURES             Location/Qualifiers
     source          1..7811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     552..571
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    570..625
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     671..690
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             1363..1374
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(1974..2177)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2003..2023)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2178..2481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3819..3836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3825..4049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4095..4523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4182..4201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4392..4413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4532..4552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4537..4866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4717..4852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4779..4798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4914..4961
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4980..5354
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5484..5605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5521..5540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5575..5594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5654..5670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5654..5670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5667..5689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5678..5694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5702..5708,5709..5726,5727..5732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5747..5768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5885..5902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6056..6644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6136..6155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6815..7606,7607..7675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7438..7457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7676..7780)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct
      781 taataaaccc aatgcctttt tcttattaat gcccaacttt gtgacccaag aaaggttggg
      841 gttagatttt cttttccatt tgcaggaaga aaacccgttt cttttaaggc agacagcagg
      901 tggatatggc caaaataggc ccccctcttg aggagccgga ggaggtacgt gtaatatgca
      961 gaggtgtttg tgagctgcag cattagtgta tcatttgcat cttttgacca attgtctttg
     1021 gggtccattg tgatgacatc atttgcatct tttgaataac tgtctttggg gtccactgtg
     1081 gtgacatctg tctttattgg aggaaagata atttcttgat caattccgtt tttattattc
     1141 tcatgtctga cgatacatct gtgttctttg tccagtgact cttctggcac cgttaaccag
     1201 ctaaatttca tgtatgtgtc gttagtcttc atggtgttcc cctcctggga gcccagaatc
     1261 gtgttgctct tcttttcttg ccaatgtatc ttaataatat ctgggaaaaa tttctcaaga
     1321 agacaaaggt atgttccagc cttctggagt tttgtttcag caattgaagg aagaaaaata
     1381 gtgggcttgg gggaaacatc tgcatcaagt tgtttatctg tgacaacaag tgttgttcca
     1441 ctgccaaaga gtttcttata tacttctcat cccaggtggc acagtaatag actccagagt
     1501 cattttcaat aagatttcgc agtatcattc tcaagttctt ccttgtgctt ccataagtat
     1561 catacttccc tgggctgatt cctgattcca acacaacgct ggaggtgtag gagtcatagt
     1621 acagaagacg ctgtggggcc ttcccctcct ggtgtagata ccagtggatg tagccggtac
     1681 ttccttcagc aagatcacaa gtgatttcag cagatgaccc agtctgcctg atgactgact
     1741 tcgttctccc ttccaagttg gaagatttct gactggcagg agacaggaaa gctagaagca
     1801 ccgctagagc ccactgcatg ccttcctctc tgaggccttg gaaggaaagg gaccagatga
     1861 agaggagcag aagatcctct taaaactcag gccccactca acaaggaagt gatccctagc
     1921 acagaagaca gtaggaggga ggatccctag cggatctgac ggttcactaa accagctctg
     1981 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     2041 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     2101 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     2161 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     2221 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     2281 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     2341 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     2401 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     2461 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     2521 attgattact attaataact actcgagtat gtcgaggtgg cgtgtacggt gggaggccta
     2581 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg aggtaccacc ggtcatatgc
     2641 cgcgggagcc cagatcctgt gtgtgtgtgt gtgtgcttgt gcacatctgt tctctgattg
     2701 gctgactgta cttcctgaga gtccctgttt aatgttacaa gaaacagctg atgcagagga
     2761 tctcctccct catccatctc tctctcttct gggcaggtaa ggcagacccc agaacttggg
     2821 ccaagcaaga ctcagcagca aagaaacatc aaggctcaca ggacctcgta ctcatggact
     2881 gtgctcctta ctcaatgacc tcagccccca gccctcactg agcccctctc ttgtgtcttt
     2941 tctctcagga gtcatgtcag ccattgagtt ggtgcctgaa caccaaacag tgcctgtgtc
     3001 aatagggatc cctgccaccc tcaggtgctc catgaaagga gaagcgatcg gtaactacta
     3061 tatcaactgg tacaggaaga cccaaggtaa cacaatgact ttcatatacc gagaaaagga
     3121 catctatggc cctggtttca aagacaattt ccaaggtgac attgatattg caaagaacct
     3181 ggctgtactt aagatacttg caccatcaga gagagatgaa gggtcttact actgtgcctg
     3241 tgaccccgta ccgggggata caacacacac aagcgacgat aaactcatct ttggaaaagg
     3301 aacccgtgtg actgtggaac caagaagtca gcctcatacc aaaccatccg tttttgtcat
     3361 gaaaaatgga acaaatgtcg cttgtctggt gaaggaattt taccccaagg atataagaat
     3421 aaatctcgtg tcatccaaga agataacaga gtttgatcct gctattgtca tctctcccgg
     3481 tgggaagtac aatgctgtca agcttggtaa atatgaagat tcaaattcag tgacatgttc
     3541 agttcaacac gacaataaaa ctgtgcactc cactgacttt gaagtgaaga cagattctac
     3601 agatcacgta aaaccaaagg aaactgaaaa cacaaagcaa ccttcaaaga gctgccataa
     3661 acccaaagcc atagttcata ccgagaaggt gaacatgatg tccctcacag tgcttgggct
     3721 acgaatgctg tttgcaaaga ctgttgccgt caattttctc ttgactgcca agttattttt
     3781 cttgtaatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4081 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4501 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4861 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     4921 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     4981 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     5041 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     5101 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     5161 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     5221 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     5281 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     5341 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     5401 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5461 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5581 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5641 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5821 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7801 cacctgacgt c
//
