LOCUS       sequence-251578-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, 
            Busino L, Rothenberg E, Pagano M
  TITLE     PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin 
            ligase to sites of DNA damage controls H2A.Z loading.
  JOURNAL   Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.
  PUBMED    29985131
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-9"; volume: "7. pii: 38771. doi"; pages: " 
            10.7554/eLife.38771"
COMMENT     Sequence Label: FLAG-HA-RNF68 [dRING]
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggactaca aggacgacga tgacaagctc gatggaggat acccntacga cgtgcccgac
       61 tacgccgaag gaagatctgc gtctcctcag gggggccaga ttgcgatcgc gatgaggctt
      121 cggaaccagc tccagtcagt gtacaagatg gacccgctac ggaacgagga ggaggttcga
      181 gtgaagatca aagacttgaa tgaacacatt gttattaaga tccacgagac acagccactg
      241 ctcaacctca aactggaccg ggtcatgcag gacatcgtgt ataagctggt gcctggcttg
      301 caagacagtg aagagaaacg gattcgggaa ttctaccagt cccgaggttt ggaccgggtc
      361 acccagccca ctggggaaga gccagcactg agcaacctcg gcctcccctt cagcagcttt
      421 gaccactcta aagcccacta ctatcgctat gatgagcagt tgaacctgtg cctggagcgg
      481 ctgagttctg gcaaagacaa gaataaaagc gtcctgcaga acaagtatgt ccgatgttct
      541 gttagagctg aggtacgcca tctccggagg gtcctgtgtc accgcttgat gctaaaccct
      601 cagcatgtgc agctcctttt tgacaatgaa gttctccctg atcacgtgac aatgaagcag
      661 atatggctct cccgctggtt cggcaagcca tcccctttgc ttttacaata cagtgtgaaa
      721 gagaagagga ggtag
//
