LOCUS       sequence-251580-        4942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Xylanase C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Furniss CS, Belshaw NJ, Alcocer MJ, Williamson G, Elliott GO, 
            Gebruers K, Haigh NP, Fish NM, Kroon PA
  TITLE     A family 11 xylanase from Penicillium funiculosum is strongly 
            inhibited by three wheat xylanase inhibitors.
  JOURNAL   Biochim Biophys Acta. 2002 Jul 29;1598(1-2):24-9.
  PUBMED    12147340
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta."; date: "2002-07-29"; volume: "1598(1-2)"; pages: 
            "24-9"
COMMENT     Sequence Label: pMJA008
FEATURES             Location/Qualifiers
     source          1..4942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2166..2186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4294..4313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4295..4313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4323..4340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4323..4339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4332..4354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4480..4936)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4618..4639)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4830..4849
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtgagtat gtaagcgagt ctgatcttcc cgcccgccaa tcaaatcgca
     2281 ggatcacaga atgcaaatac cttctgatcc caaccacaac cagtatttct gattcgaatc
     2341 aaaatcgtgg catgtaattc gcgggatcag aatgcggttt ccaccggcca aaatgaccga
     2401 tctgcgttgc cacggctggt aatggcagga tcagagcagc agcgttgaat cagtcagatc
     2461 aagcgccgct cccgctgatt ggccagatca cccactagcg cgtgatccca ggcaccttcc
     2521 cgcgtccggc tctttctata gaaatatctc ccgccgcgtc ttccttccca ccttctttct
     2581 cctctccatc tccagctcca tcttcaagct tactcaacat tatcaacgcg tttacgcatc
     2641 cacattcaat tacttatcat ctcatcccca tacagaattc aaaatgaagc tcttcctagc
     2701 tgcaattgtc ctttgcgcaa ctgccgcgac agccttccca tcagaacttg ctcaacgcgc
     2761 tgcgggagac cttagcaagc gtcaatcaat cacgaccagc cagactggga cgaacaacgg
     2821 ctactactac tcgttctgga ccaacggcgg aggagaagtc acttacacaa atggcgacaa
     2881 tggcgagtac agcgtgacat gggtcgattg tggtgacttt acatctggca agggctggaa
     2941 tccagccaat gcacagtaag tttccctctt tccttctaag cttatattgt acgtactcac
     3001 aatttgcagg actgtcacgt actcaggaga atttaacccc tctggaaacg cttatttggc
     3061 tgtctacggg tggacaacag atcctcttgt cgaatactac atcctggagt cctacggcac
     3121 ctataaccca tcatctggac ttacttcact tggccaggtc actagcgatg gtggcaccta
     3181 cgatatctac tcaacccagc gtgtcaacca gccttccatt gagggaactt ccaccttcaa
     3241 ccagtactgg tcagttcgca ccgagaagcg agtcggcgga actgtcacca cggccaacca
     3301 ctttgcagca tggaaggcac ttggacttga aatgggcact tataactata tgattgtgtc
     3361 caccgaaggc tacgagagca gtggctctag taccatcaca gtgtcctaga catgtctcaa
     3421 tgacgcttgt tacacagctg tccctttatt gacacttata aatgacttat ggaagggagt
     3481 cggcaaaatt tttatgttcg aagtttcata tgtctattgt ggaaatcggc ccatattttc
     3541 agggctagtc actctttgat tgcatcttaa gttacttgat caaattaagc cctaacacca
     3601 agatctggaa tgcgagcaat atcaagtatt tattcattta ttttaaaccc cggagtgggc
     3661 tgtctttgat agtatagtaa tgatgcacat ttgttgtggc agccttacct gtttttccat
     3721 tggcattcga gatatctacc gacatgttcc ttcagcaagc agtatttatc gcgtctcgat
     3781 caagcatcga cggccttttg gggaaaccaa gaaaaatatt ttggcctcca tatctctgtc
     3841 gcacattccc tccttctctg aaacctttgc ttttgggaac gttcgaaaaa acagagcggt
     3901 tgcaagcagt agctccatcc aggcaagatg cataccgatg catactagtg agtaggccag
     3961 ttagcgaatt gtttgttctc agtgccgatg atgaaattat gcaattaaag acttactgcg
     4021 agacccgcca ccaaagggca tgaaaacatg cttcatctct tttgtgggat tctcccatct
     4081 gcttggatca aagctatatc ccggacatca atagttagcg atattgaatc gaacatctgc
     4141 catgccttgt aggcgggaaa gtgacaccga ataggctata ggaaccactc acgcatgagg
     4201 attgggaaac acatcagggt cgcgatgtaa agtatatgcc tgagtagata ctgtgacacc
     4261 tccagctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4321 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4381 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4441 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt
     4501 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt tttttaacca ataggccgaa
     4561 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4621 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4681 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     4741 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4801 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4861 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4921 ccgctacagg gcgcgtcagg tg
//
