LOCUS       sequence-251596-        8967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pLENTI_RPS3_Keima
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..635
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     661..680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        661..679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1475..1477,1478..1480,1481..2143)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             2162..2191
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2210..2233
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2267..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2290..2842
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2842..3441
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2842..2861)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3338..3358
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(3471..3504)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3560..4148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3613..3633)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4031..4042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4151..4167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4152..4168)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4677..4857
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4919..5507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4999..5018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5584..6243)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6158..6177
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6244..6348)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6423..6442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6613..6992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6994..7192
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7210..7390
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7437..7562
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8059..8292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8477..8521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8819..8936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tttggttagt accgggcccg ctctagacat gtccaatatg accgccatgt tgacattgat
       61 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      241 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      301 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      361 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
      421 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
      481 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      541 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
      601 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattttg
      661 taatacgact cactataggg cggccgggaa ttcgtcgact ggatccggta ccgaggagat
      721 ctgccgccgc gatcgccggc gcgccatggc agtgcaaata tccaagaaga ggaagtttgt
      781 cgctgatggc atcttcaaag ctgaactgaa tgagtttctt actcgggagc tggctgaaga
      841 tggctactct ggagttgagg tgcgagttac accaaccagg acagaaatca ttatcttagc
      901 caccagaaca cagaatgttc ttggtgagaa gggccggcgg attcgggaac tgactgctgt
      961 agttcagaag aggtttggct ttccagaggg cagtgtagag ctttatgctg aaaaggtggc
     1021 cactagaggt ctgtgtgcca ttgcccaggc agagtctctg cgttacaaac tcctaggagg
     1081 gcttgctgtg cggagggcct gctatggtgt gctgcggttc atcatggaga gtggggccaa
     1141 aggctgcgag gttgtggtgt ctgggaaact ccgaggacag agggctaaat ccatgaagtt
     1201 tgtggatggc ctgatgatcc acagcggaga ccctgttaac tactacgttg acactgctgt
     1261 gcgccacgtg ttgctcagac agggtgtgct gggcatcaag gtgaagatca tgctgccctg
     1321 ggacccaact ggtaagattg gccctaagaa gcccctgcct gaccacgtga gcattgtgga
     1381 acccaaagat gagatactgc ccaccacccc catctcagaa cagaagggtg ggaagccaga
     1441 gccgcctgcc atgccccagc cagtccccac agcaatggtg agtgtgatcg ctaaacaaat
     1501 gacctacaag gtttatatgt caggcacggt caatggacac tactttgagg tcgaaggcga
     1561 tggaaaagga aagccttacg agggagagca gacagtaaag ctcactgtca ccaagggtgg
     1621 acctctgcca tttgcttggg atattttatc accacagctt cagtacggaa gcataccatt
     1681 caccaagtac cctgaagaca tccctgatta tttcaagcag tcattccctg agggatatac
     1741 atgggagagg agcatgaact ttgaagatgg tgcagtgtgt actgtcagca atgattccag
     1801 catccaaggc aactgtttca tctacaatgt caaaatctct ggtgagaact ttcctcccaa
     1861 tggacctgtt atgcagaaga agacacaggg ctgggaaccc agcactgagc gtctctttgc
     1921 acgagatgga atgctgatag gaaacgatta tatggctctg aagttggaag gaggtggtca
     1981 ctatttgtgt gaatttaaat ctacttacaa ggcaaagaag cctgtgagga tgccagggcg
     2041 ccacgagatt gaccgcaaac tggatgtaac cagtcacaac agggattaca catctgttga
     2101 gcagtgtgaa atagccattg cacgccactc tttgctcggt taaacgcgta cgcggccgct
     2161 cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg attacaagga
     2221 tgacgacgat aaggtttaaa cctaggcgta gcggccgcaa attccgcccc tctccctccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     2881 gacgacgtcc cccgggcagt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     2941 cgccacaccg tcgatccaga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     3001 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     3061 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     3121 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     3181 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     3241 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     3301 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     3361 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     3421 acccgcaagc ccggtgcctg atgtacaagt aggattcgtc gagggaccta ataacttcgt
     3481 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     3541 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3601 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3661 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3721 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3781 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3841 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3901 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3961 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4021 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4081 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4141 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     4201 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     4261 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     4321 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4381 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     4441 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     4501 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     4561 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     4621 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     4681 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4741 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4801 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     4861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5341 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5581 tgattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     5641 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     5701 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     5761 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     5821 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     5881 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     5941 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     6001 ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat
     6061 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     6121 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     6181 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     6241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6361 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     6421 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     6481 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     6541 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     6601 agatatcgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     6661 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     6721 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     6781 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     6841 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     6901 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     6961 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7021 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7081 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7141 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     7201 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7381 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7441 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7501 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7561 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7621 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7681 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7741 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7801 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7861 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     7921 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7981 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8041 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8101 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8161 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8221 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8281 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8341 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8401 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8461 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8521 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8581 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8641 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     8701 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     8761 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     8821 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8881 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8941 tttattacag ggacagcaga gatccag
//
