LOCUS       sequence-251598-        8966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pLENTI_RPS3_K214R_Keima
FEATURES             Location/Qualifiers
     source          1..8966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     204..223
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        204..222
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1017..1019,1020..1022,1023..1685)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             1704..1733
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1752..1775
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1809..2382
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1832..2384
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1976..1993)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2203..2222
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2384..2983
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2384..2403)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2880..2900
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(3013..3046)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3102..3690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3155..3175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3573..3584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3693..3709)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3694..3710)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4219..4399
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4461..5049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4541..4560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5126..5785)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5700..5719
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5786..5890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5965..5984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6155..6534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6536..6734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6752..6932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6979..7104
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7601..7834
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8019..8063
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8361..8478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8561..8940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8941..8966,1..178)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
       61 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
      121 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      181 tttagtgaac cgtcagaatt ttgtaatacg actcactata gggcggccgg gaattcgtcg
      241 actggatccg gtaccgagga gatctgccgc cgcgatcgcc ggcgcgcatg gcagtgcaaa
      301 tatccaagaa gaggaagttt gtcgctgatg gcatcttcaa agctgaactg aatgagtttc
      361 ttactcggga gctggctgaa gatggctact ctggagttga ggtgcgagtt acaccaacca
      421 ggacagaaat cattatctta gccaccagaa cacagaatgt tcttggtgag aagggccggc
      481 ggattcggga actgactgct gtagttcaga agaggtttgg ctttccagag ggcagtgtag
      541 agctttatgc tgaaaaggtg gccactagag gtctgtgtgc cattgcccag gcagagtctc
      601 tgcgttacaa actcctagga gggcttgctg tgcggagggc ctgctatggt gtgctgcggt
      661 tcatcatgga gagtggggcc aaaggctgcg aggttgtggt gtctgggaaa ctccgaggac
      721 agagggctaa atccatgaag tttgtggatg gcctgatgat ccacagcgga gaccctgtta
      781 actactacgt tgacactgct gtgcgccacg tgttgctcag acagggtgtg ctgggcatca
      841 aggtgaagat catgctgccc tgggacccaa ctggtaagat tggccctaag aagcccctgc
      901 ctgaccacgt gagcattgtg gaacccagag atgagatact gcccaccacc cccatctcag
      961 aacagaaggg tgggaagcca gagccgcctg ccatgcccca gccagtcccc acagcaatgg
     1021 tgagtgtgat cgctaaacaa atgacctaca aggtttatat gtcaggcacg gtcaatggac
     1081 actactttga ggtcgaaggc gatggaaaag gaaagcctta cgagggagag cagacagtaa
     1141 agctcactgt caccaagggt ggacctctgc catttgcttg ggatatttta tcaccacagc
     1201 ttcagtacgg aagcatacca ttcaccaagt accctgaaga catccctgat tatttcaagc
     1261 agtcattccc tgagggatat acatgggaga ggagcatgaa ctttgaagat ggtgcagtgt
     1321 gtactgtcag caatgattcc agcatccaag gcaactgttt catctacaat gtcaaaatct
     1381 ctggtgagaa ctttcctccc aatggacctg ttatgcagaa gaagacacag ggctgggaac
     1441 ccagcactga gcgtctcttt gcacgagatg gaatgctgat aggaaacgat tatatggctc
     1501 tgaagttgga aggaggtggt cactatttgt gtgaatttaa atctacttac aaggcaaaga
     1561 agcctgtgag gatgccaggg cgccacgaga ttgaccgcaa actggatgta accagtcaca
     1621 acagggatta cacatctgtt gagcagtgtg aaatagccat tgcacgccac tctttgctcg
     1681 gttaaacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     1741 atgatatcct ggattacaag gatgacgacg ataaggttta aacctaggcg tagcggccgc
     1801 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     1861 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     1921 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     1981 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2041 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2101 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2161 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2221 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2281 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2341 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatgaccg agtacaagcc
     2401 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggca gtacgcaccc tcgccgccgc
     2461 gttcgccgac taccccgcca cgcgccacac cgtcgatcca gaccgccaca tcgagcgggt
     2521 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2581 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2641 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2701 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     2761 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2821 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2881 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     2941 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatgtacaa gtaggattcg
     3001 tcgagggacc taataacttc gtatagcata cattatacga agttatacat gtttaagggt
     3061 tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     3121 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3181 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3241 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3301 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3361 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3421 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3481 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     3541 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3601 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3661 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
     3721 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     3781 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     3841 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3901 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     3961 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     4021 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     4081 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     4141 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     4201 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4261 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4321 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4381 gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5101 tcacgttaag ggattttggt catgattacg ccccgccctg ccactcatcg cagtactgtt
     5161 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     5221 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     5281 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     5341 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     5401 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     5461 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     5521 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaac tccggatgag
     5581 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     5641 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     5701 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     5761 tatatccagt gatttttttc tccatactct tcctttttca atattattga agcatttatc
     5821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5881 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     5941 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6001 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     6061 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     6121 gctgcttcgc gatgtacggg ccagatatcg cgttgacatt gattattgac tagttattaa
     6181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     6241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     6421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     6541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     6601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     6661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     6721 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     6781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6901 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6961 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7021 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7081 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7141 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7201 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7261 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7321 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7381 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7441 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7501 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7561 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7621 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7681 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7741 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7801 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7861 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7921 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7981 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8041 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8101 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8161 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8221 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8281 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     8341 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     8401 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     8461 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta
     8521 ccgggcccgc tctagacatg tccaatatga ccgccatgtt gacattgatt attgactagt
     8581 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8641 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8821 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8881 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8941 gtgatgcggt tttggcagta caccaa
//
