LOCUS       sequence-251599-        8967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pLENTI_RPS3_K227R_Keima
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    213..330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        413..792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        793..996
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1836..1838,1839..1841,1842..2504)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             2523..2552
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2571..2594
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2628..3201
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2651..3203
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2795..2812)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3022..3041
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3203..3802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3203..3222)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3699..3719
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(3832..3865)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3921..4509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3974..3994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4392..4403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4512..4528)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4513..4529)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5038..5218
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5280..5868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5360..5379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5945..6604)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6519..6538
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6605..6709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6784..6803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6974..7353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7355..7553
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7571..7751
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7798..7923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8420..8653
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8838..8882
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
       61 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
      121 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
      181 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      241 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      301 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      361 gtttggttag taccgggccc gctctagaca tgtccaatat gaccgccatg ttgacattga
      421 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      661 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      721 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
      781 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
      841 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      901 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
      961 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt
     1021 gtaatacgac tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga
     1081 tctgccgccg cgatcgccgg cgcgccatgg cagtgcaaat atccaagaag aggaagtttg
     1141 tcgctgatgg catcttcaaa gctgaactga atgagtttct tactcgggag ctggctgaag
     1201 atggctactc tggagttgag gtgcgagtta caccaaccag gacagaaatc attatcttag
     1261 ccaccagaac acagaatgtt cttggtgaga agggccggcg gattcgggaa ctgactgctg
     1321 tagttcagaa gaggtttggc tttccagagg gcagtgtaga gctttatgct gaaaaggtgg
     1381 ccactagagg tctgtgtgcc attgcccagg cagagtctct gcgttacaaa ctcctaggag
     1441 ggcttgctgt gcggagggcc tgctatggtg tgctgcggtt catcatggag agtggggcca
     1501 aaggctgcga ggttgtggtg tctgggaaac tccgaggaca gagggctaaa tccatgaagt
     1561 ttgtggatgg cctgatgatc cacagcggag accctgttaa ctactacgtt gacactgctg
     1621 tgcgccacgt gttgctcaga cagggtgtgc tgggcatcaa ggtgaagatc atgctgccct
     1681 gggacccaac tggtaagatt ggccctaaga agcccctgcc tgaccacgtg agcattgtgg
     1741 aacccaaaga tgagatactg cccaccaccc ccatctcaga acagaggggt gggaagccag
     1801 agccgcctgc catgccccag ccagtcccca cagcaatggt gagtgtgatc gctaaacaaa
     1861 tgacctacaa ggtttatatg tcaggcacgg tcaatggaca ctactttgag gtcgaaggcg
     1921 atggaaaagg aaagccttac gagggagagc agacagtaaa gctcactgtc accaagggtg
     1981 gacctctgcc atttgcttgg gatattttat caccacagct tcagtacgga agcataccat
     2041 tcaccaagta ccctgaagac atccctgatt atttcaagca gtcattccct gagggatata
     2101 catgggagag gagcatgaac tttgaagatg gtgcagtgtg tactgtcagc aatgattcca
     2161 gcatccaagg caactgtttc atctacaatg tcaaaatctc tggtgagaac tttcctccca
     2221 atggacctgt tatgcagaag aagacacagg gctgggaacc cagcactgag cgtctctttg
     2281 cacgagatgg aatgctgata ggaaacgatt atatggctct gaagttggaa ggaggtggtc
     2341 actatttgtg tgaatttaaa tctacttaca aggcaaagaa gcctgtgagg atgccagggc
     2401 gccacgagat tgaccgcaaa ctggatgtaa ccagtcacaa cagggattac acatctgttg
     2461 agcagtgtga aatagccatt gcacgccact ctttgctcgg ttaaacgcgt acgcggccgc
     2521 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg
     2581 atgacgacga taaggtttaa acctaggcgt agcggccgca aattccgccc ctctccctcc
     2641 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2701 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2761 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2821 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     2881 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     2941 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3001 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3061 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3121 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3181 ctttgaaaaa cacgatgata atatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3241 cgacgacgtc ccccgggcag tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3301 gcgccacacc gtcgatccag accgccacat cgagcgggtc accgagctgc aagaactctt
     3361 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3421 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3481 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3541 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3601 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3661 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3721 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3781 gacccgcaag cccggtgcct gatgtacaag taggattcgt cgagggacct aataacttcg
     3841 tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat
     3901 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3961 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4021 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4081 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4141 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4201 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4261 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4321 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4381 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4441 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4501 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     4561 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     4621 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     4681 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4741 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     4801 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     4861 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     4921 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     4981 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     5041 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5101 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5161 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     5221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5281 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5341 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5401 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5461 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5521 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5581 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5641 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5701 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5761 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5821 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5881 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5941 atgattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     6001 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     6061 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     6121 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     6181 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     6241 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     6301 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     6361 gctcaccgtc tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa
     6421 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     6481 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     6541 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     6601 ccatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6721 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     6781 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     6841 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     6901 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     6961 cagatatcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     7021 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     7081 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     7141 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     7201 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     7261 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     7321 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     7381 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     7441 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     7501 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     7561 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7741 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7801 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7861 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7921 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7981 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8041 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     8101 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     8161 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     8221 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     8281 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8341 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8401 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8461 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8521 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8581 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8641 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8701 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8761 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8821 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8881 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8941 taggaggctt ggtaggttta agaatag
//
