LOCUS       sequence-251607-         337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enhanced RNAi vector for C. elegans. Contains two T7 polymerase 
            termination sequences adjacent to the T7 promoter sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
  TITLE     Highly efficient RNAi and Cas9-based auto-cloning systems for C. 
            elegans research.
  JOURNAL   Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 
            10.1093/nar/gky516.
  PUBMED    29924347
REFERENCE   2  (bases 1 to 337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516."
COMMENT     Sequence Label: T444T
FEATURES             Location/Qualifiers
     source          1..337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(118..165)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     166..185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        166..184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     242..258
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     242..258
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcaa
      121 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagtaata cgactcacta
      181 tagggagacc ggcagatctg atatcatcga tgaattcgag ctccaccgcg gtggcggccg
      241 ctctagaact agtggatcca ccggttccat ggctagccac gtgacgcgtg gatcccccgg
      301 gctgcaggaa ttcgatatca agcttatcga taccgtc
//
