LOCUS       sequence-251614-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pLENTI_RPS2_Keima
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    306..423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        506..885
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        886..1089
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1115..1134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1115..1133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2079..2081,2082..2084,2085..2747)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             2766..2795
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2814..2837
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2871..3444
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2894..3446
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3038..3055)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3265..3284
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3446..4045
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3446..3465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3942..3962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4075..4108)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4164..4752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4217..4237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4635..4646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4755..4771)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4756..4772)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5281..5461
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5523..6111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5603..5622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6188..6847)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6762..6781
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6848..6952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7027..7046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7217..7596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7598..7796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7814..7994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8041..8166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8663..8896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(9081..9117,1..8)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
       61 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
      121 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      181 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      241 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      301 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      361 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      421 tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg cccgctctag
      481 acatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
      541 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      601 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      661 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      721 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
      781 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
      841 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      901 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      961 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1021 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1081 agcagagctc gtttagtgaa ccgtcagaat tttgtaatac gactcactat agggcggccg
     1141 ggaattcgtc gactggatcc ggtaccgagg agatctgccg ccgcgatcgc cggcgcgcca
     1201 tggcggatga cgccggtgca gcgggggggc ccgggggccc tggtggccct gggatgggga
     1261 accgcggtgg cttccgcgga ggtttcggca gtggcatccg gggccggggt cgcggccgtg
     1321 gacggggccg gggccgaggc cgcggagctc gcggaggcaa ggccgaggat aaggagtgga
     1381 tgcccgtcac caagttgggc cgcttggtca aggacatgaa gatcaagtcc ctggaggaga
     1441 tctatctctt ctccctgccc attaaggaat cagagatcat tgatttcttc ctgggggcct
     1501 ctctcaagga tgaggttttg aagattatgc cagtgcagaa gcagacccgt gccggccagc
     1561 gcaccaggtt caaggcattt gttgctatcg gggactacaa tggccacgtc ggtctgggtg
     1621 ttaagtgctc caaggaggtg gccaccgcca tccgtggggc catcatcctg gccaagctct
     1681 ccatcgtccc cgtgcgcaga ggctactggg ggaacaagat cggcaagccc cacactgtcc
     1741 cttgcaaggt gacaggccgc tgcggctctg tgctggtacg cctcatccct gcacccaggg
     1801 gcactggcat cgtctccgca cctgtgccta agaagctgct catgatggct ggtatcgatg
     1861 actgctacac ctcagcccgg ggctgcactg ccaccctggg caacttcgcc aaggccacct
     1921 ttgatgccat ttctaagacc tacagctacc tgacccccga cctctggaag gagactgtat
     1981 tcaccaagtc tccctatcag gagttcactg accacctcgt caagacccac accagagtct
     2041 ccgtgcagcg gactcaggct ccagctgtgg ctacaacaat ggtgagtgtg atcgctaaac
     2101 aaatgaccta caaggtttat atgtcaggca cggtcaatgg acactacttt gaggtcgaag
     2161 gcgatggaaa aggaaagcct tacgagggag agcagacagt aaagctcact gtcaccaagg
     2221 gtggacctct gccatttgct tgggatattt tatcaccaca gcttcagtac ggaagcatac
     2281 cattcaccaa gtaccctgaa gacatccctg attatttcaa gcagtcattc cctgagggat
     2341 atacatggga gaggagcatg aactttgaag atggtgcagt gtgtactgtc agcaatgatt
     2401 ccagcatcca aggcaactgt ttcatctaca atgtcaaaat ctctggtgag aactttcctc
     2461 ccaatggacc tgttatgcag aagaagacac agggctggga acccagcact gagcgtctct
     2521 ttgcacgaga tggaatgctg ataggaaacg attatatggc tctgaagttg gaaggaggtg
     2581 gtcactattt gtgtgaattt aaatctactt acaaggcaaa gaagcctgtg aggatgccag
     2641 ggcgccacga gattgaccgc aaactggatg taaccagtca caacagggat tacacatctg
     2701 ttgagcagtg tgaaatagcc attgcacgcc actctttgct cggttaaacg cgtacgcggc
     2761 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     2821 aggatgacga cgataaggtt taaacctagg cgtagcggcc gcaaattccg cccctctccc
     2881 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     2941 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     3001 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     3061 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     3121 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     3181 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     3241 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     3301 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     3361 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     3421 ttcctttgaa aaacacgatg ataatatgac cgagtacaag cccacggtgc gcctcgccac
     3481 ccgcgacgac gtcccccggg cagtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3541 cacgcgccac accgtcgatc cagaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3601 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3661 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3721 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3781 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     3841 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     3901 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     3961 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4021 catgacccgc aagcccggtg cctgatgtac aagtaggatt cgtcgaggga cctaataact
     4081 tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc cactaggtac
     4141 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4741 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc
     4801 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     4861 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     4921 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4981 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     5041 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     5101 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     5161 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     5221 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     5281 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5341 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5401 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5461 agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5701 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5941 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6181 gtcatgatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     6241 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     6301 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     6361 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     6421 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     6481 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     6541 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     6601 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     6661 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     6721 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     6781 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     6841 tctccatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6901 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6961 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     7021 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     7081 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     7141 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     7201 ggccagatat cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7261 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7321 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7381 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7441 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7501 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     7561 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     7621 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     7681 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     7741 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     7801 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7861 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7921 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7981 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8041 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8101 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8161 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8221 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8281 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8341 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8401 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8461 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     8521 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8581 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8641 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8701 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8761 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8821 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8881 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8941 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     9001 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     9061 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatggg
//
