LOCUS       sequence-251616-        7868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses nut lipid binding TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  TITLE     Alcocer Lab plasmids
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMJA297
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     552..571
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    570..625
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     671..690
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     promoter        complement(1974..2177)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2003..2023)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2178..2481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3876..3893)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3882..4106
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4152..4580
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4239..4258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4449..4470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4589..4609)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4594..4923
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4774..4909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4836..4855
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4971..5018
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5037..5411
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5541..5662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5578..5597)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5632..5651
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5711..5727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5711..5727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5724..5746)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5735..5751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5759..5765,5766..5783,5784..5789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5804..5825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5942..5959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6113..6701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6193..6212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6872..7663,7664..7732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7495..7514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7733..7837)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct
      781 agcctctgga atcctttctc ttgaccatgg ccatcagcac gagggcactg accagcacgg
      841 catacaaggt ggccttccct agcaagatct catagaggat ggtggcagac aggacccctt
      901 gctggtaaga ctcggaggtg aagccacagt ctgctctacc ccaggcctcg gcgctgacga
      961 tctgggtgac aggtttggcc ctatcctggg tccactcgtc attctccgag agcccgtaga
     1021 actggacttg acagcggaag tggttgcggg ggttctgcca gaaggtggcc gagaccctca
     1081 ggcggctgct caggcagtat ctggagtcat tgagggcggg ctgctccttg aggggctgcg
     1141 ggtctgtgct gaccccactg tgcacctcct tcccattcac ccaccagctc agctccacgt
     1201 ggtcggggta gaagcctgtg gccaggcaca ccagtgtggc cttttgggtg tgggagatct
     1261 ctgcttctga tggctcaaac acagcgacct cgggtgggaa cacgtttttc aggtcctcga
     1321 gcactgtcag ccgggtgcct gggccaaaat actgcgtatc caccgagccc ccctgagggc
     1381 tgctggcaca gaagtacaca gatgtctgag agggtacagc agacgccaac gtgaggggga
     1441 aatcatctgt gtttgctctg gagacactat aaccatcagg gacttctcct ttgccagtgg
     1501 tacctgcagt atttgaataa tggatgagcc ttagccccag tcctagatct tgtctatacc
     1561 agtacatggc attatgtctc atatcctggg tacatctcag tgtcatgcgc cgtcctgctg
     1621 ccaggatctg agatgttggt gcctgggtga tcccagcaat cactggacct acagagaagg
     1681 agaacaaagc caatgctgca gcctcaatgg gaaggctctg ggtcttgaaa tctacacatg
     1741 ggccctgccc aggacttacc tgcccacagg agagaaaagg ccacacagca caggagcctg
     1801 attctcatga cagcatcccc ttgttcaggg tccaggactt ctgggggaac tatttggagg
     1861 tttggatctg tagtctccta ataggctctg ctcgacaagc ttatcgatgc ggccgcgcta
     1921 gcacgcgtca gctgactaga ggatccctag cggatctgac ggttcactaa accagctctg
     1981 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     2041 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     2101 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     2161 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     2221 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     2281 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     2341 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     2401 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     2461 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     2521 attgattact attaataact actcgagtat gtcgaggtgg cgtgtacggt gggaggccta
     2581 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg aggtaccacc ggtcatatgc
     2641 cgcgggagcc cagatcctgt gtgtgtgtgt gtgtgcttgt gcacatctgt tctctgattg
     2701 gctgactgta cttcctgaga gtccctgttt aatgttacaa gaaacagctg ccatgtgggg
     2761 agttttcctt ctttatgttt ccatgaagat gggaggtaag tctcaatcta atagcaaatg
     2821 ctgctgggag ttttcaaaac gattttcttt cagctaaatg attgccattt ctgacactcg
     2881 taacaaaagt gtttcttaat agatattttc tttatgcggt gctaattgtt attggatatc
     2941 aaataataac gactttgctg ccatttattt ctaagaatct ctccttaatg gcaattcttt
     3001 tgtgaccaca tggagacatc gtttttaatg ataatgggtt tccaggagcg ctgccctagt
     3061 aacagtcatg tatcttgtct taggcactac aggacaaaac attgaccagc ccactgagat
     3121 gacagctacg gaaggtgcca ttgtccagat caactgcacg taccagacat ctgggctcaa
     3181 cgggctgttc tggtaccagc aacatgctgg cgaagcaccc acatttctgt cttacaatgt
     3241 tctggatggt ttggagggga aaggtcgttt ttcttcattc cttagtcggt ctaaagggta
     3301 cagttacctc cttttgaagg agctccagat gaaagactct gcctcttacc tctgtgcagg
     3361 ggtggatagc agctataaat tgatcttcgg agcaggaaca agactatttg ttaaagcaaa
     3421 tatccagaac cctgaccctg ccgtgtacca gctgagagac tctaaatcca gtgacaagtc
     3481 tgtctgccta ttcaccgatt ttgattctca aacaaatgtg tcacaaagta aggattctga
     3541 tgtgtatatc acagacaaaa ctgtgctaga catgaggtct atggacttca agagcaacag
     3601 tgctgtggcc tggagcaaca aatctgactt tgcatgtgca aacgccttca acaacagcat
     3661 tattccagaa gacaccttct tccccagccc agaaagttcc tgtgatgtca agctggtcga
     3721 gaaaagcttt gaaacagata cgaacctaaa ctttcaaaac ctgtcagtga ttgggttccg
     3781 aatcctcctc ctgaaagtgg ccgggtttaa tctgctcatg acgctgcggc tgtggtccag
     3841 ctgatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3901 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3961 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4021 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4081 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4141 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4201 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4261 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4321 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4381 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4441 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4501 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4561 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4681 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4741 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4801 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4861 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4921 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     4981 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     5041 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     5101 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     5161 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     5221 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     5281 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     5341 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     5401 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5461 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5521 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5581 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5641 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5701 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5761 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5821 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5881 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5941 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6001 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6061 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6121 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6181 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6241 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6301 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6361 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6421 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6481 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6541 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6601 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6661 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6721 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6781 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6841 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6901 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6961 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7021 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7081 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7141 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7201 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7261 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7321 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7381 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7441 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7501 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7561 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7621 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7681 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7741 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7801 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7861 ctgacgtc
//
