LOCUS       sequence-251621-        8762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optical reporter of presynaptic ATP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Rangaraju V, Calloway N, Ryan TA
  TITLE     Activity-driven local ATP synthesis is required for synaptic 
            function.
  JOURNAL   Cell. 2014 Feb 13;156(4):825-35. doi: 10.1016/j.cell.2013.12.042.
  PUBMED    24529383
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "825-35"
COMMENT     Sequence Label: Syn-ATP
FEATURES             Location/Qualifiers
     source          1..8762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        495..698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     649..669
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     743..762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        743..761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1785..2492
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1930..1948)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2198..2217
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2392..2415
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2508..4160
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRALCVRFSHA
                     RDPIFGNQIAPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFLAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPKGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDLAYWDEDEHFFIVGRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2574..2592)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(4195..4213)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4196..4213)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4233..4250)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4239..4463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4509..4937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4596..4615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4806..4827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4946..4966)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4951..5280
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5131..5266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5193..5212
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5347..6141
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5401..5420)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6011..6030
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6315..6436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6352..6371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6406..6425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6485..6501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6485..6501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6498..6520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6509..6525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6533..6539,6540..6557,6558..6563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6578..6599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6716..6733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6887..7475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6967..6986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7646..8437,8438..8506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8269..8288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8507..8611)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8686..8705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
       61 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      121 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      181 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      481 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      541 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      601 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      661 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      721 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      781 gagctcggat ccgcatcgaa ttcggggcag cagcaatgga cgtggtgaat cagctggtgg
      841 ctgggggtca gttccgggtg gtcaaggagc cccttggctt cgtgaaggtg ctgcagtggg
      901 tctttgccat cttcgccttt gctacgtgtg gcagctacac cggggagctt cggctgagcg
      961 tggagtgtgc caacaagacg gagagtgccc tcaacatcga agttgaattc gagtacccct
     1021 tcaggctgca ccaagtgtac tttgatgcac cctcctgcgt caaagggggc actaccaaga
     1081 tcttcctggt tggggactac tcctcgtcgg ctgaattctt tgtcaccgtg gctgtgtttg
     1141 ccttcctcta ctccatgggg gccctggcca cctacatctt cctgcagaac aagtaccgag
     1201 agaacaacaa agggcctatg atggactttc tggctacagc cgtgttcgct ttcatgtggc
     1261 tagttagttc atcagcctgg gccaaaggcc tgtccgatgt gaagatggcc acggacccag
     1321 agaacattat caaggagatg cccatgtgcc gccagacagg gaacacatgc aaggaactga
     1381 gggaccctgt gacttcagga ctcaacacct cagtggtgtt tggcttcctg aacctggtgc
     1441 tctgggttgg caacttatgg ttcgtgttca aggagacagg ctgggcagcc ccattcatgc
     1501 gcgcacctcc aggcgccccg gaaaagcaac cagcacctgg cgatgcctac ggcgatgcgg
     1561 gctacgggca gggccccgga ggctatgggc cccaggactc ctacgggcct cagggtggtt
     1621 atcaacccga ttacgggcag ccagccagcg gtggcggtgg ctacgggcct cagggcgact
     1681 atgggcagca aggctatggc caacagggtg cgcccacctc cttctccaat cagatgggat
     1741 ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcgatggtg agcaagggcg
     1801 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1861 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1921 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     1981 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2041 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2101 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2161 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2221 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2281 tcaagcagag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     2341 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca
     2401 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     2461 actccaccgg cggcatggac gagctgtaca agggaccggt gctagcgatg gaagacgcca
     2521 aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc
     2581 aactgcataa ggctatgaag agatacgctc tggttcctgg aacaattgct tttacagatg
     2641 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
     2701 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     2761 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     2821 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
     2881 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
     2941 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     3001 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
     3061 cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac
     3121 ctaagggtgt ggcccttccg catagagctc tctgcgtcag attctcgcac gccagagatc
     3181 caatatttgg caatcaaatc gctccggata ctgcgatttt aagtgttgtt ccattccatc
     3241 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     3301 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
     3361 cgttgctagt accaacccta ttttcattct tggccaaaag tactctgatt gacaaatacg
     3421 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
     3481 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
     3541 ctactagtgc tattctgatt acacccaagg gggatgataa accgggcgcg gtcggtaaag
     3601 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     3661 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
     3721 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacctagctt
     3781 actgggacga agacgaacac ttcttcatag ttggccgctt gaagtcttta attaaataca
     3841 aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct
     3901 tcgacgcagg tgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     3961 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     4021 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     4081 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     4141 gcggaaagat cgccgtgtaa gcggccgctc gagcatgcat ctagagggcc ctattctata
     4201 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     4261 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4321 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4381 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4441 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4501 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4861 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4921 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4981 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5041 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5101 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5161 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     5221 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5281 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     5341 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5401 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5461 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5521 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5581 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5641 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5701 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5761 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5821 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5881 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5941 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6001 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6061 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6121 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6181 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6241 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6301 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6361 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6421 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7201 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7261 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7321 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7381 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7441 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7501 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7561 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7621 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7681 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7741 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7801 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7861 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7921 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7981 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8041 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8101 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8161 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8221 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8281 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8341 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8401 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8461 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8521 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8581 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8641 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8701 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     8761 cg
//
