LOCUS       sequence-251622-        3318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human RelA as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_023
FEATURES             Location/Qualifiers
     source          1..3318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      131..719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     620..639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2112..2483
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     terminator      complement(2503..2537)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2608..3267)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3182..3201
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat tctgacgaac
      841 tgttccccct catcttcccg gcagagccag cccaggcctc tggcccctat gtggagatca
      901 ttgagcagcc caagcagcgg ggcatgcgct tccgctacaa gtgcgagggg cgctccgcgg
      961 gcagcatccc aggcgagagg agcacagata ccaccaagac ccaccccacc atcaagatca
     1021 atggctacac aggaccaggg acagtgcgca tctccctggt caccaaggac cctcctcacc
     1081 ggcctcaccc ccacgagctt gtaggaaagg actgccggga tggcttctat gaggctgagc
     1141 tctgcccgga ccgctgcatc cacagtttcc agaacctggg aatccagtgt gtgaagaagc
     1201 gggacctgga gcaggctatc agtcagcgca tccagaccaa caacaacccc ttccaagttc
     1261 ctatagaaga gcagcgtggg gactacgacc tgaatgctgt gcggctctgc ttccaggtga
     1321 cagtgcggga cccatcaggc aggcccctcc gcctgccgcc tgtcctttct catcccatct
     1381 ttgacaatcg tgcccccaac actgccgagc tcaagatctg ccgagtgaac cgaaactctg
     1441 gcagctgcct cggtggggat gagatcttcc tactgtgtga caaggtgcag aaagaggaca
     1501 ttgaggtgta tttcacggga ccaggctggg aggcccgagg ctccttttcg caagctgatg
     1561 tgcaccgaca agtggccatt gtgttccgga cccctcccta cgcagacccc agcctgcagg
     1621 ctcctgtgcg tgtctccatg cagctgcggc ggccttccga ccgggagctc agtgagccca
     1681 tggaattcca gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa
     1741 ggacatatga aaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc
     1801 cccggcctcc acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc
     1861 cagcacccca gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc
     1921 ccaccatggt gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc
     1981 cccaagtcct gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg
     2041 cccaggcccc agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac
     2101 ctgcccccaa gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc
     2161 agtttgatga tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca
     2221 cagacctggc atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg
     2281 tggcccccca cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag
     2341 tgacaggggc ccagaggcca ccagacccag ctcctgctcc actgggggcc ccggggctcc
     2401 ccaatggcct cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag
     2461 ccctgctgag tcagatcagc tccggatcct gagaccagac caataaaaaa cgcccggcgg
     2521 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2581 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     2641 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2701 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2761 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2821 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2881 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2941 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     3001 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     3061 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     3121 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     3181 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     3241 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     3301 ctcaaaaaat acgcccgg
//
