LOCUS       sequence-251624-        2436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes synTF fused to VP16 activation domain as a Type 3b part to 
            be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_025
FEATURES             Location/Qualifiers
     source          1..2436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      121..709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     610..629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1358..1591
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      complement(1611..1645)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1716..2375)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2290..2309
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
       61 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      781 gttatcccct gattctgtgg ataaccgtag tcggtctcat tcttctagac caggcgaacg
      841 accgtttcaa tgccggatat gtatgaggaa cttctccgag gaggcaaact tgaggcgcca
      901 cacccgaaca catacaggag aaaagccatt ccaatgtcga atttgtatgc gcaatttttc
      961 agatcactca agcctcaagc gacacctccg cacacatact ggttcacaga agccctttca
     1021 gtgcaggatt tgtatgcgca actttagcca atcagcgaac cttttgcggc acactagaac
     1081 gcatacaggt gagaagcctt tccagtgtcg catctgtatg cggaacttca gcgaccccag
     1141 ttcattgaag aggcatttgc gaactcacac cggttctcaa aaaccttttc agtgccgaat
     1201 ttgtatgcgc aacttcagcc aacaaacaaa tttgacgaga cacacgcgca cgcacaccgg
     1261 ggaaaaaccg tttcaatgtc gaatctgcat gcgcaatttt agcgatgcga cacaacttgt
     1321 taggcatctg cgcacacact tgcggggatc cggtagtgcc cccccgaccg atgtcagcct
     1381 gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga
     1441 cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca
     1501 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     1561 cgatgccctt ggaattgacg agtacggtgg gggatcctga gaccagacca ataaaaaacg
     1621 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1681 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1741 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1801 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1861 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1921 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     1981 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2041 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2101 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     2161 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2221 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2281 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2341 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2401 ctcgataact caaaaaatac gcccggtagt gatctt
//
