LOCUS       sequence-251625-        2352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes synTF fused to VP64 activation domain as a Type 3b part to 
            be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_026
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      122..710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     611..630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1359..1508
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      complement(1528..1562)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1633..2292)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2207..2226
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
       61 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      241 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      481 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      781 cgttatcccc tgattctgtg gataaccgta gtcggtctca ttcttctaga ccaggcgaac
      841 gaccgtttca atgccggata tgtatgagga acttctccga ggaggcaaac ttgaggcgcc
      901 acacccgaac acatacagga gaaaagccat tccaatgtcg aatttgtatg cgcaattttt
      961 cagatcactc aagcctcaag cgacacctcc gcacacatac tggttcacag aagccctttc
     1021 agtgcaggat ttgtatgcgc aactttagcc aatcagcgaa ccttttgcgg cacactagaa
     1081 cgcatacagg tgagaagcct ttccagtgtc gcatctgtat gcggaacttc agcgacccca
     1141 gttcattgaa gaggcatttg cgaactcaca ccggttctca aaaacctttt cagtgccgaa
     1201 tttgtatgcg caacttcagc caacaaacaa atttgacgag acacacgcgc acgcacaccg
     1261 gggaaaaacc gtttcaatgt cgaatctgca tgcgcaattt tagcgatgcg acacaacttg
     1321 ttaggcatct gcgcacacac ttgcggggat ccggtagtga tgctttagac gattttgact
     1381 tagatatgct tggttcagac gcgttagacg acttcgacct agacatgtta ggctcagatg
     1441 cattggacga cttcgattta gatatgttgg gctccgatgc cctagatgac tttgatctag
     1501 atatgctagg atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1561 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1621 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1681 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1741 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1801 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
     1861 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1921 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1981 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2041 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     2101 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2161 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2221 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2281 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2341 cggtagtgat ct
//
