LOCUS       sequence-251657-        5926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Left arm of TALEN pair 1 for cleavage of apobb.1 STOP locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Thierer JH, Ekker SC, Farber SA
  TITLE     The LipoGlo reporter system for sensitive and specific monitoring of
            atherogenic lipoproteins.
  JOURNAL   Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w.
  PUBMED    31366908
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-31"; volume: "10(1):3426. doi"; pages: " 
            10.1038/s41467-019-11259-w"
COMMENT     Sequence Label: pT3TS-TAL-apobb.1-PR1-LA
FEATURES             Location/Qualifiers
     source          1..5926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        146..164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           173..215
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             285..305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             306..332
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             2631..3218
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     3'UTR           3257..3382
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3481..3500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3482..3500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3507..3524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3507..3523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3516..3538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3691..4119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3801..3822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4013..4032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4146..4250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4251..4319,4320..5111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4469..4488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5282..5870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5771..5790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgaatta accctcacta aagggaagct tgcttgttct
      181 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaactcgag aaagatattg
      241 tatatatcgt aacaatagga ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa
      301 aggttagttg gaaggacgca agtggttggt ctagagtgga tctacgcacg ctcggctaca
      361 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
      421 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
      481 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
      541 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
      601 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
      661 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
      721 cactgacggg tgcccccctg aacctgaccc cggaccaagt ggtggctatc gccagcaaca
      781 atgsmggcwr rcrrgygcyc smmwsraysy grccccggac caagtggtgg ctatcgccag
      841 caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
      901 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca
      961 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     1021 ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca
     1081 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     1141 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     1201 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagcc acgatggcgg
     1261 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     1321 cacacccgat caggtcgtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
     1381 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     1441 ggctatcgcc agcmacgrtg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     1501 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagccacga
     1561 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     1621 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     1681 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     1741 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     1801 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     1861 caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     1921 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     1981 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
     2041 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
     2101 gcggctgttg ccggtgctgt gccaggacca tggcctgacy ccggaccaag tggtggctat
     2161 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     2221 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acattggcgg
     2281 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     2341 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaag
     2401 cattgtggcc cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca acgaccacct
     2461 cgysgcsttg gccngcstyg rcsracgwcc wscymgtcgc cttggcctgc ctcggcggac
     2521 gtcctgccat ggatgcagtg aaaaagggat tgccgcacgc gccggaattg atcagaagag
     2581 tcaatcgccg tattggcgaa cgcacgtccc atcgcgttgc ctctagatcc cagctagtga
     2641 aatctgaatt ggaagagaag aaatctgaac ttagacataa attgaaatat gtgccacatg
     2701 aatatattga attgattgaa atcgcaagaa attcaactca ggatagaatc cttgaaatga
     2761 aggtgatgga gttctttatg aaggtttatg gttatcgtgg taaacatttg ggtggatcaa
     2821 ggaaaccaga cggagcaatt tatactgtcg gatctcctat tgattacggt gtgatcgttg
     2881 atactaaggc atattcagga ggttataatc ttccaattgg tcaagcagat gaaatgcaaa
     2941 gatatgtcga agagaatcaa acaagaaaca agcatatcaa ccctaatgaa tggtggaaag
     3001 tctatccatc ttcagtaaca gaatttaagt tcttgtttgt gagtggtcat ttcaaaggaa
     3061 actacaaagc tcagcttaca agattgaatc atatcactaa ttgtaatgga gctgttctta
     3121 gtgtagaaga gcttttgatt ggtggagaaa tgattaaagc tggtacattg acacttgagg
     3181 aagtgagaag gaaatttaat aacggtgaga taaactttta ataggctagt gactgactag
     3241 gatctggtta ccactaaacc agcctcaaga acacccgaat ggagtctcta agctacataa
     3301 taccaactta cactttacaa aatgttgtcc cccaaaatgt agccattcgt atctgctcct
     3361 aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaamm
     3421 cccccccccc ccccctgcag gtcgactcta ctagaggatc cccgggcgag ctccaattcg
     3481 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     3541 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3661 yskramkygy mmkskwkmat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3721 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3781 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3841 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3901 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3961 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4021 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4081 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccatkcsmcw ttymggskrm
     4141 rtgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4261 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4321 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4381 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4441 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4501 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4561 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4621 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4681 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4741 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4801 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4861 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4921 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4981 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5041 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5101 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5161 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5221 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5281 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5341 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5401 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5461 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5521 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5581 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5641 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5701 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5761 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5821 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5881 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctca
//
