LOCUS       sequence-251665-        4185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSM040
FEATURES             Location/Qualifiers
     source          1..4185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             281..955
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    1008..1028
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(1166..1200)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1275..1934)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1849..1868
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(2286..3236)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3284..3506
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4035..4046
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4093..4114
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaagaaga
      121 caaaaatcac cttgcgctaa gagctcgaca ccatcgaatg gtgcaaaacc tttcgcggta
      181 tggcatgata gcgcccggaa gcatgccact taaaaaggag atcaacaatg aaagcaattt
      241 tcgtactgaa acatcttaat catgctgggg agggtttcta atggcttcct ccgaagacgt
      301 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      361 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      421 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      481 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      541 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      601 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      661 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      721 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      781 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      841 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      901 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgctacctc
      961 cgcagcgaac gacgaaaatt acgcccttgc agcgtaataa agagacctac tagtagcggc
     1021 cgctgcagtt aatcactgat taactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1081 gggggccttt tttcgttttg gtccttttgt tatcaataaa aaaggggagc ggtttcccgc
     1141 tccccttatt gttcgtctac aaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1201 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1261 cgatatcatt taaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1321 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1381 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1441 ttgtccatat tggccacatt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1501 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1561 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1621 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1681 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1741 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1801 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1861 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1921 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1981 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     2041 ttaaatgagt ttcagttcca caggtaccca gaaggtgctt ccaagttaca ttgtcgatct
     2101 gttcatggtg aacagctttg aatacaccaa aaactcgtaa aagctctgat gtatctatct
     2161 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa
     2221 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
     2281 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
     2341 gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt
     2401 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
     2461 ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
     2521 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
     2581 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
     2641 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
     2701 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
     2761 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
     2821 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
     2881 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
     2941 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
     3001 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
     3061 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
     3121 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
     3181 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
     3241 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
     3301 ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga
     3361 aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt
     3421 gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga
     3481 atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg
     3541 atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt
     3601 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3661 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3721 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3781 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3841 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct ggccacttcg
     3901 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     3961 caacaggctt acccgtctta ctgtcacgga aaaaaaaacc ccgcccctga cagggcgggg
     4021 ttttttttcc aattattgaa ggccgctaac gcggcctttt tttgtttctg gtctgcctta
     4081 atcaatgact aagaattcgc ggccgcttct agagggtctc ggagtccacc tcgagctttc
     4141 aggaaaattt ttctgtataa tagattcata aatttgagag aagag
//
