LOCUS       sequence-251667-        3869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - helper plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSM038
FEATURES             Location/Qualifiers
     source          1..3869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             55..66
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             91..804
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFDGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             814..870
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(1941..2732)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2733..2824)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2872..3610)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ttcgttacca attgacatga tacgaaacgt accgtatcgt taaggttact agagaaagag
       61 gagaaatttt tcaggtcgga ggtcgtagat atgcgtaaag gcgaagagct gttcactggt
      121 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt
      181 ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact
      241 ggtaaactgc cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc
      301 tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa
      361 ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg
      421 gaagtgaaat ttgacggcga taccctggta aaccgcattg agctgaaagg cattgacttt
      481 aaagaagacg gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt
      541 tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac
      601 gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat
      661 ggtcctgttc tgctgccaga caatcactat ctgagctacc aaagcgttct gtctaaagat
      721 ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg
      781 catggtatgg atgaactgta caaaggttcc ggtgctacta acttctctct gctgaaacag
      841 gctggtgacg ttgaagaaaa cccgggtccg ggatctatgc ttatcttatt aggttacctg
      901 gttgttctcg gtacagtttt cggcggttat ttgatgaccg gtggaagcct tggagcactc
      961 tatcaacccg ctgaactggt gattattgcc ggtgcaggga ttgggtcgtt tatcgtcggc
     1021 aataatggca aagcgattaa aggcacgctg aaggcgctgc cgttgctgtt tcgtcgctcc
     1081 aaatacacca aagcaatgta tatggatctg ctggctctgc tttatcggtt gatggcgaaa
     1141 tcgcggcaga tggggatgtt ttcgctggaa cgtgatattg aaaatccccg tgagagcgag
     1201 atcttcgcca gctacccacg catcctcgcg gatagcgtca tgcttgattt tatcgtcgat
     1261 tatctgcgcc tgattatcag cggtcacatg aacaccttcg aaatcgaagc tctgatggat
     1321 gaagagattg agacgcacga aagcgaggca gaagtcccgg cgaacagtct ggcgctggtc
     1381 ggggactcac ttccggcgtt tggtattgtt gcggctgtaa tgggggtcgt tcacgcgtta
     1441 ggttcagccg atcgtcctgc cgccgagctg ggtgcgctta tcgcacatgc gatggtgggg
     1501 actttcctcg gcattttatt ggcttacgga tttatttccc cattagcgac tgttttacgt
     1561 cagaaaagcg ccgaaaccag caaaatgatg cagtgcgtca aagtcactct gctttctaat
     1621 ctgaacggtt acgcaccgcc tatcgccgtt gagtttggtc gcaaaacgct ctattccagc
     1681 gaacgtccgt cgtttattga actggaagag catgtgcgtg cggtgaaaaa tccgcaacaa
     1741 cagacgacaa ccgaggaagc atgagtgtct tcaccataat gaatactttc gctttctaga
     1801 tagctgagta gcctagagct agagtgcgcg acaggtttga tgacaaaaaa ttagcctatt
     1861 taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg
     1921 cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     1981 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
     2041 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     2101 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     2161 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     2221 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     2281 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     2341 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     2401 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     2461 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     2521 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     2581 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     2641 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2701 gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata ttattgaagc
     2761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2821 caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
     2881 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
     2941 acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     3001 taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     3061 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc
     3121 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta
     3181 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
     3241 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
     3301 cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc
     3361 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     3421 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     3481 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     3541 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     3601 tcctttgatc ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt
     3661 agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc
     3721 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     3781 gcctaatgag tgagctaact tacattaatt gcgttgcgcc acagaagaga gtcaattcag
     3841 ggtggtgaat catcaatccg gtaggtttt
//
