LOCUS       sequence-251671-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Studies of cellular contractility.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, 
            Liu B, Nixon BR, Manalo AE, Becker JR, Crawley SW, Bader DM, Tyska 
            MJ, Liu Q, Gutzman JH, Burnette DT
  TITLE     Muscle-specific stress fibers give rise to sarcomeres in 
            cardiomyocytes.
  JOURNAL   Elife. 2018 Dec 12;7. pii: 42144. doi: 10.7554/eLife.42144.
  PUBMED    30540249
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-12-12"; volume: "7. pii: 42144. doi"; pages: " 
            10.7554/eLife.42144"
COMMENT     Sequence Label: pHalo-MyosinIIA-C25
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      605..614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             611..1501
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    7617..7738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7654..7673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7708..7727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7745..8200)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7882..7903)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8094..8113
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8227..8331
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8333..8690
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8333..8524
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8356..8376)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8541..8676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8725..9519
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8725..9519
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8779..8798)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9389..9408
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9707..9726)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9751..9798
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10127..10715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10616..10635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg ttagtgaccg ctcgagccgc tagcgctacc
      601 ggtcgccacc atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt
      661 cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt
      721 cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc
      781 gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct
      841 gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct
      901 ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa
      961 gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac
     1021 ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt
     1081 cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt
     1141 cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt
     1201 tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa
     1261 catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct
     1321 gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa
     1381 aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga
     1441 caacccggac ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg
     1501 cgagccaacc actgaggact tttccggact cagatctggc agcggtggag gcagcgcatc
     1561 cggcggaagc ggaagcgctc agcaggctgc agacaagtac ctctatgtgg ataaaaactt
     1621 catcaataac ccgctggccc aagctgactg ggctgccaag aagttggtat gggtgccttc
     1681 cagcaagaat ggctttgaac cagccagcct caaggaggag gtgggagaag aggccattgt
     1741 agagctggta gagaatggga agaaggtgaa ggtgaacaag gacgacatcc agaagatgaa
     1801 cccacccaag ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcttc
     1861 ggtgctgcac aacctcaagg agcgatacta ctcagggctt atctacacct attcaggcct
     1921 gttctgtgtg gtcatcaacc cttataagaa cctgcccatc tactcagagg agatcgtgga
     1981 gatgtacaag ggcaagaaga ggcacgagat gccaccccac atctacgcca tcacagatac
     2041 tgcctaccgg agcatgatgc aggaccggga agatcagtcc atcctgtgca cgggggagtc
     2101 tggagcaggg aagacagaga acaccaagaa agtcatccag tacctggcac atgtggcctc
     2161 ctcacacaag agcaagaagg accaggggga gttggagcgg cagctgctac aggccaaccc
     2221 tatcctagag gcctttggaa acgccaagac ggtgaagaat gacaactcct ctcgattcgg
     2281 taaattcatt cgtatcaact ttgatgtcaa tggctacatt gttggtgcca acattgagac
     2341 ttatcttctg gagaaatctc gtgctatccg ccaagccaaa gaggagcgga ccttccacat
     2401 cttctactac ctgctgtctg gggccggaga acacctgaag actgatctcc tgttggagcc
     2461 atacaacaaa taccgcttcc tgtccaacgg gcacgtcacc atccctgggc agcaggacaa
     2521 ggacatgttc caggagacaa tggaggccat gagaattatg ggtatcccag aggatgagca
     2581 gatgggcttg ctgcgggtca tctctggggt ccttcagctt ggcaacattg ccttcaagaa
     2641 ggagcggaac actgaccagg cgtccatgcc ggacaacaca gctgctcaaa aggtgtccca
     2701 cctcctgggg atcaatgtga ccgacttcac cagaggcatc ctcaccccac gcatcaaggt
     2761 gggcagagac tatgtgcaga aggcgcagac taaagagcag gctgactttg ccattgaggc
     2821 cttggccaag gctacctatg agcggatgtt ccgctggctg gtgcttcgca tcaacaaagc
     2881 tctggacaag accaagaggc agggcgcctc atttatcggg atactggaca tcgctggctt
     2941 tgaaatcttt gatctgaact ccttcgagca gctgtgcatc aactacacca acgagaagct
     3001 gcagcagctg ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgagaggg
     3061 catcgagtgg aacttcatcg acttcggcct ggacctgcag ccctgcatcg acctcattga
     3121 gaagccggcg ggtcccccag gcatcctggc cctgctagat gaggagtgct ggtttcctaa
     3181 ggccactgac aagagcttcg tggagaaggt ggtgcaggag cagggcaccc accccaagtt
     3241 ccagaagccc aagcaactga aggacaaggc tgatttctgc attatccact atgccggcaa
     3301 ggtggactat aaagctgacg agtggctgat gaagaacatg gaccccttga acgacaacat
     3361 cgccacgctg cttcaccagt cctcagacaa gtttgtctct gagctgtgga aggatgtgga
     3421 tcggatcatt ggcttggacc aagtggctgg aatgtccgag acagcactac ctggtgcctt
     3481 caagacccgg aagggcatgt tccgtactgt cggacagctg tacaaggagc agctggccaa
     3541 gctcatggcc acgttgagga ataccaaccc caacttcgtg cgctgcatca ttcccaacca
     3601 tgagaagaag gccggcaaac tggacccgca cttggtgctg gaccagctgc gctgcaatgg
     3661 cgtccttgag ggcatccgga tctgccgcca gggctttccc aacagggtgg tcttccagga
     3721 gttccggcag aggtatgaga tcctcacccc caactccatc ccgaagggct tcatggatgg
     3781 caagcaagcg tgtgtgctca tgatcaaagc cttggagctt gacagcaacc tgtaccgcat
     3841 cggccagagc aaagtgttct tccgggcagg agtgctcgcc cacctggagg aagagcggga
     3901 cctgaagatc accgatgtca tcattggctt ccaggcctgc tgcaggggct acctggccag
     3961 gaaggccttt gccaagaggc agcaacagct gaccgccatg aaggtcctac agaggaactg
     4021 tgctgcgtac ctcaggctgc gcaactggca gtggtggagg ctcttcacca aggtcaagcc
     4081 cctgttgaac tcaataagac atgaggatga gctgttagcc aaggaggcgg aactgacaaa
     4141 ggttcgagag aaacatctgg ctgcagagaa caggctgaca gagatggaga cgatgcagtc
     4201 tcagctcatg gcagagaagc tgcagcttca ggagcagctg caggcggaga cagagctgtg
     4261 tgccgaggct gaggagctcc gggcccgtct gacagcgaag aagcaggagc tggaggagat
     4321 atgccatgac ctggaggcca gggtggagga ggaggaggag cgctgccagt acctgcaggc
     4381 cgagaagaag aagatgcagc agaacatcca ggaacttgag gagcagttgg aggaggagga
     4441 gagcgcccgg cagaagctgc agcttgagaa ggtgaccacc gaggccaagc tgaagaaact
     4501 ggaggaggac cagatcatca tggaggacca gaactgcaaa ctggccaagg agaagaaact
     4561 gctggaagac agagtagctg agttcactac caacctcatg gaagaggagg agaagtccaa
     4621 gagcctggcc aagctcaaga acaagcacga ggcaatgatc accgacctgg aagagcgcct
     4681 ccgtagggag gagaagcaga ggcaggagtt ggagaagacc cgtcgcaagc tggagggaga
     4741 ctccacagac ctcagtgacc agattgctga gctccaggcg cagatagcag agctcaagat
     4801 gcagctggcc aagaaggagg aggagttgca ggctgccttg gccagagtgg aagaagaagc
     4861 tgctcagaag aatatggccc tgaagaagat ccgagaactg gaaactcaga tatctgagct
     4921 ccaggaggac ctggagtcgg agcgagcctc caggaataaa gccgagaagc agaaacggga
     4981 tctgggagag gagctggagg cgctgaagac agagctggag gacacgctgg actccacggc
     5041 tgcccagcag gagctgaggt cgaagcgtga gcaggaggtg agcatcctga agaagactct
     5101 ggaggacgag gccaagaccc atgaggccca gatccaggag atgaggcaga agcactcaca
     5161 ggctgtggag gagctggcag atcagttgga gcagacgaag cgggtaaaag ctacccttga
     5221 gaaggcgaag cagaccctgg agaatgagcg gggagagctg gccaatgagg tgaaggccct
     5281 gctgcaaggc aagggcgact cagagcacaa gcgcaagaag gtggaggcgc agctgcaaga
     5341 actgcaggtc aagttcagcg agggagagcg cgtgcgaacc gaactggccg acaaggtcac
     5401 caagctgcag gttgaactgg acagtgtgac cggtctcctt agccagtctg acagcaagtc
     5461 cagcaagctt acgaaggact tctctgcgct ggagtcccag cttcaggaca cacaggagtt
     5521 gctccaggag gagaaccggc agaagctgag cctgagcacc aagctcaagc agatggagga
     5581 tgagaaaaac tccttcaggg agcagctgga ggaggaggag gaggccaagc gcaacttgga
     5641 gaagcagatc gccacgctcc atgcccaggt gaccgacatg aagaagaaga tggaggacgg
     5701 tgtagggtgc ctggagactg cagaggaggc gaagcggagg cttcagaagg acttggaagg
     5761 cctgagccag cggcttgagg agaaggtggc tgcctacgat aagctggaga agaccaagac
     5821 acggctgcag caggagctgg acgacctgct ggttgacctg gaccaccagc ggcagagcgt
     5881 ctccaacctg gaaaagaagc agaagaagtt cgaccagctc ctagccgagg agaagaccat
     5941 ctcggccaag tatgcagagg agcgtgaccg agctgaggct gaggcccgtg agaaggagac
     6001 aaaggcgcta tcactggccc gggcgcttga ggaggccatg gagcagaagg cagagctgga
     6061 gcggctcaac aagcagttcc gcacggagat ggaggacctc atgagctcca aggatgacgt
     6121 gggcaagagt gtccacgagc tggagaagtc caagcgggcc ttggagcagc aggtggagga
     6181 gatgaagacc cagctggagg agctggagga tgagctgcag gccacggagg atgccaagct
     6241 ccgcctggag gtgaacctgc aggccatgaa ggcccagttt gagcgggatc tgcagggccg
     6301 ggatgaacag agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc
     6361 ggagctggag gatgagagga agcagcgctc catggccatg gccgcacgca agaaactgga
     6421 gatggatctg aaggacctgg aggcacacat tgacacagcc aataagaacc gggaagaggc
     6481 catcaaacag ctgcggaagc ttcaggccca gatgaaggac tgcatgcggg agctggacga
     6541 cacgcgcgcc tcccgggagg agatcctggc gcaggccaag gagaatgaga agaagctgaa
     6601 gagcatggag gccgagatga ttcagctgca ggaggaactg gcagctgctg agcgtgctaa
     6661 gcgtcaggcc caacaggaac gggacgagct ggctgatgag atcgccaaca gcagtggcaa
     6721 aggggcccta gcattagagg agaagcggcg actggaggcc cgcattgccc tgctggagga
     6781 ggagctggag gaggaacagg gcaacacgga gctgatcaac gatcggctga agaaggccaa
     6841 cctgcagatc gaccaaataa acaccgacct gaacctggaa cgcagccacg cacagaagaa
     6901 tgagaatgcg cgacagcagc tggaacgcca gaacaaggag ctcaaggcca agctgcagga
     6961 aatggagagt gctgtcaagt ccaaatacaa ggcctccatc gcggccttgg aggccaaaat
     7021 tgcacagctg gaggaacagc tggacaacga gaccaaggag cgccaggcag cctccaagca
     7081 ggtgcgccgg acggagaaga agctgaagga cgtgctgctg caggtggagg acgagcggag
     7141 gaacgcggaa cagttcaagg accaggctga caaggcgtcc acccgcctga agcagcttaa
     7201 acggcagcta gaggaggctg aagaggaggc ccagcgggcc aatgcctcac gccggaagct
     7261 gcagcgtgag ctggaagatg ccacagagac cgctgatgct atgaaccgcg aggtcagctc
     7321 cctgaagaac aaactgaggc gtggggacct gccatttgtc gtgactcgcc gaattgttcg
     7381 gaaaggcact ggcgactgct cagacgagga ggtcgacggt aaagcagatg gggccgatgc
     7441 caaggcagct gaataggaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     7501 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     7561 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     7621 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7681 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     7741 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7801 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7861 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7921 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7981 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8041 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8101 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8161 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     8221 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8281 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     8341 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8461 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8521 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8581 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8641 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     8701 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     8761 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     8821 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     8881 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     8941 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     9001 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     9061 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     9121 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     9181 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     9241 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     9301 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     9361 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     9421 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     9481 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     9541 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     9601 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9661 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     9721 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     9781 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     9841 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     9901 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     9961 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10021 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10081 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10141 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10201 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10261 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10321 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10381 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10441 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10501 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10561 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10621 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10681 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10741 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10801 ctgtggataa ccgtattacc gccatgcat
//
