LOCUS       sequence-251674-        3343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pAA6
FEATURES             Location/Qualifiers
     source          1..3343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             281..955
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1138..1165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1332..1471
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1387..1406
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1486..1503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1657..2244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1737..1756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2293..2952)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2867..2886
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3266..3287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaagaaga
      121 caaaaatcac cttgcgctaa gagctcgaca ccatcgaatg gtgcaaaacc tttcgcggta
      181 tggcatgata gcgcccggaa gcatgccact taaaaaggag atcaacaatg aaagcaattt
      241 tcgtactgaa acatcttaat catgctgggg agggtttcta atggcttcct ccgaagacgt
      301 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      361 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      421 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      481 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      541 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      601 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      661 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      721 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      781 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      841 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      901 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgctacctc
      961 cgcagcgaac gacgaaaatt acgcccttgc agcgtaataa ctacgtgctg ctgaagttgc
     1021 ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc
     1081 atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc tcacactggc
     1141 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     1201 tattaatccg gcttttttat tatttcctag ctagccaacg catatgagac ggtcacagct
     1261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1321 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     1381 actatgcggc atcagagcag attgtactga gagtgcacca ttgcggtgtg aaataccgca
     1441 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     1501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2221 tacgcgcaga aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacagc
     2281 ttggtctgac agttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     2341 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     2401 cagcaccttg tcgccttgcg tataatattt gcccatcgtg aaaacggggg cgaagaagtt
     2461 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     2521 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2581 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2641 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2701 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     2761 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2821 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2881 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2941 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     3001 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactt
     3061 accaatgctg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3121 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3181 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3241 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccacctc gagctttcag
     3301 gaaaattttt ctgtataata gattcataaa tttgagagaa gag
//
