LOCUS       sequence-251676-       10863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Studies of cellular contractility..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, 
            Liu B, Nixon BR, Manalo AE, Becker JR, Crawley SW, Bader DM, Tyska 
            MJ, Liu Q, Gutzman JH, Burnette DT
  TITLE     Muscle-specific stress fibers give rise to sarcomeres in 
            cardiomyocytes.
  JOURNAL   Elife. 2018 Dec 12;7. pii: 42144. doi: 10.7554/eLife.42144.
  PUBMED    30540249
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-12-12"; volume: "7. pii: 42144. doi"; pages: " 
            10.7554/eLife.42144"
COMMENT     Sequence Label: pHalo-MyosinIIB-C25
FEATURES             Location/Qualifiers
     source          1..10863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        375..578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      617..626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             623..1513
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    7661..7782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7698..7717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7752..7771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7789..8244)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7926..7947)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8138..8157
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8271..8375
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8377..8734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8377..8568
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8400..8420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8585..8720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8769..9563
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8769..9563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8823..8842)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9433..9452
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9751..9770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9795..9842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10171..10759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10660..10679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      601 gctagcgcta ccggtcgcca ccatggcaga aatcggtact ggctttccat tcgaccccca
      661 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
      721 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
      781 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
      841 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
      901 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
      961 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     1021 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     1081 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
     1141 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
     1201 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
     1261 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
     1321 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     1381 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     1441 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct
     1501 cgagatttcc ggcgagccaa ccactgagga cttttccgga ggtagcgcat ccagtggggg
     1561 tgcgtcaggc agtggcagcg gatctggcgc gcagagaact ggactcgagg atccagagag
     1621 gtatctcttt gtggacaggg ctgtcatcta caaccctgcc actcaagctg attggacagc
     1681 taaaaagcta gtgtggattc catcagaacg ccatggtttt gaggcagcta gtatcaaaga
     1741 agaacgggga gatgaagtta tggtggagtt ggcagagaat ggaaagaaag caatggtcaa
     1801 caaagatgat attcagaaga tgaacccacc taagttttcc aaggtggagg atatggcaga
     1861 attgacatgc ttgaatgaag cttccgtttt acataatctg aaggatcgct actattcagg
     1921 actaatctat acttattctg gactcttctg tgtagttata aacccttaca agaatcttcc
     1981 aatttactct gagaatatta ttgaaatgta cagagggaag aagcgtcatg agatgcctcc
     2041 acacatctat gctatatctg aatctgctta cagatgcatg cttcaagatc gtgaggacca
     2101 gtcaattctt tgcacgggtg agtcaggtgc tgggaagaca gaaaatacaa agaaagttat
     2161 tcagtacctt gcccatgttg cttcttcaca taaaggaaga aaggaccata atattcctgg
     2221 ggaacttgaa cggcagcttt tgcaagcaaa tccaattctg gaatcatttg gaaatgcgaa
     2281 gactgtgaaa aatgataact catctcgttt tggcaaattt attcggatca actttgatgt
     2341 aactggctat atcgttgggg ccaacattga aacatacctt ctggaaaagt ctcgtgctgt
     2401 tcgtcaagca aaagatgaac gtacttttca tatcttttac cagttgttat ctggagcagg
     2461 agaacaccta aagtctgatt tgcttcttga aggatttaat aactacaggt ttctctccaa
     2521 tggctatatt cctattccgg gacagcaaga caaagataat ttccaggaga ccatggaagc
     2581 aatgcacata atgggcttct cccatgaaga gattctgtca atgcttaaag tagtatcttc
     2641 agtgctacag tttggaaata tttctttcaa aaaggagaga aatactgatc aagcttccat
     2701 gccagaaaat acagttgcgc agaagctctg ccatcttctt gggatgaatg tgatggagtt
     2761 tactcgggcc atcctgactc cccggatcaa ggtcggccga gactatgtgc aaaaagccca
     2821 gaccaaagaa caggcagatt ttgcagtaga agcattggca aaagctacct atgagcggct
     2881 ctttcgctgg ctcgttcatc gcatcaataa agctctggat aggaccaaac gtcagggagc
     2941 atctttcatt ggaatcctgg atattgctgg atttgaaatt tttgagctga actcctttga
     3001 acaactttgc atcaactaca ccaatgagaa gctgcagcag ctgttcaacc acaccatgtt
     3061 tatcctagaa caagaggaat accagcgcga aggcatcgag tggaacttca tcgatttcgg
     3121 gctggatctg cagccatgca tcgacctaat agagagacct gcgaaccctc ctggtgtact
     3181 ggcccttttg gatgaagaat gctggttccc taaagccaca gataaaacct ttgttgaaaa
     3241 actggttcaa gagcaaggtt cccactccaa gtttcagaaa cctcgacaat taaaagacaa
     3301 agctgatttt tgcattatac attatgcagg gaaggtggac tataaggcag atgagtggct
     3361 gatgaagaat atggaccccc tgaatgacaa cgtggccacc cttttgcacc agtcatcaga
     3421 cagatttgtg gcagagcttt ggaaagatgt ggaccgtatc gtgggtctgg atcaagtcac
     3481 tggtatgact gagacagctt ttggctccgc atataaaacc aagaagggca tgtttcgtac
     3541 cgttgggcaa ctctacaaag aatctctcac caagctgatg gcaactctcc gaaacaccaa
     3601 ccctaacttt gttcgttgta tcattccaaa tcacgagaag agggctggaa aattggatcc
     3661 acacctagtc ctagatcagc ttcgctgtaa tggtgtcctg gaagggatcc gaatctgtcg
     3721 ccagggcttc cctaaccgaa tagttttcca ggaattcaga cagagatatg agatcctaac
     3781 tccaaatgct attcctaaag gttttatgga tggtaaacag gcctgtgaac gaatgatccg
     3841 ggctttagaa ttggacccaa acttgtacag aattggacag agcaagatat ttttcagagc
     3901 tggagttctg gcacacttag aggaagaaag agatttaaaa atcaccgata tcattatctt
     3961 cttccaggcc gtttgcagag gttacctggc cagaaaggcc tttgccaaga agcagcagca
     4021 actaagtgcc ttaaaggtct tgcagcggaa ctgtgccgcg tacctgaaat tacggcactg
     4081 gcagtggtgg cgagtcttca caaaggtgaa gccgcttcta caagtgactc gccaggagga
     4141 agaacttcag gccaaagatg aagagctgtt gaaggtgaag gagaagcaga caaaggtgga
     4201 aggagagctg gaggagatgg agcggaagca ccagcagctt ttagaagaga agaatatcct
     4261 tgcagaacaa ctacaagcag agactgagct ctttgctgaa gcagaagaga tgagggcaag
     4321 acttgctgct aaaaagcagg aattagaaga gattctacat gacttggagt ctagggttga
     4381 agaagaagaa gaaagaaacc aaatcctcca aaatgaaaag aaaaaaatgc aagcacatat
     4441 tcaggacctg gaagaacagc tagacgagga ggaaggggct cggcaaaagc tgcagctgga
     4501 aaaggtgaca gcagaggcca agatcaagaa gatggaagag gagattctgc ttctcgagga
     4561 ccaaaattcc aagttcatca aagaaaagaa actcatggaa gatcgcattg ctgagtgttc
     4621 ctctcagctg gctgaagagg aagaaaaggc gaaaaacttg gccaaaatca ggaataagca
     4681 agaagtgatg atctcagatc tagaagaacg cttaaagaag gaagaaaaga ctcgtcagga
     4741 actggaaaag gccaaaagaa aactcgacgg ggagacgacc gacctgcagg accagatcgc
     4801 agagctgcag gcgcagattg atgagctcaa gctgcagctg gccaagaagg aggaggagct
     4861 gcagggcgca ctggccagag gtgatgatga aacactccat aagaacaatg cccttaaagt
     4921 tgtgcgagag ctacaagccc aaattgctga acttcaggaa gactttgaat ccgagaaggc
     4981 ttcacggaac aaggccgaaa agcagaaaag ggacttgagt gaggaactgg aagctctgaa
     5041 aacagagctg gaggacacgc tggacaccac ggcagcccag caggaactac gtacaaaacg
     5101 tgaacaagaa gtggcagagc tgaagaaagc tcttgaggag gaaactaaga accatgaagc
     5161 tcaaatccag gacatgagac aaagacacgc aacagccctg gaggagctct cagagcagct
     5221 ggaacaggcc aagcggttca aagcaaatct agagaagaac aagcagggcc tggagacaga
     5281 taacaaggag ctggcgtgtg aggtgaaggt cctgcagcag gtcaaggctg agtctgagca
     5341 caagaggaag aagctcgacg cgcaggtcca ggagctccat gccaaggtct ctgaaggcga
     5401 caggctcagg gtggagctgg cggagaaagc aagtaagctg cagaatgagc tagataatgt
     5461 ctccaccctt ctggaagaag cagagaagaa gggtattaaa tttgctaagg atgcagctag
     5521 tcttgagtct caactacagg atacacagga gcttcttcag gaggagacac gccagaaact
     5581 aaacctgagc agtcggatcc ggcagctgga agaggagaag aacagtcttc aggagcagca
     5641 ggaggaggag gaggaggcca ggaagaacct ggagaagcaa gtgctggccc tgcagtccca
     5701 gttggctgat accaagaaga aagtagatga cgacctggga acaattgaaa gtctggaaga
     5761 agccaagaag aagcttctga aggacgcgga ggccctgagc cagcgcctgg aggagaaggc
     5821 actggcgtat gacaaactgg agaagaccaa gaaccgcctg cagcaggagc tggacgacct
     5881 cacggtggac ctggaccacc agcgccaggt cgcctccaac ttggagaaga agcagaagaa
     5941 gtttgaccag ctgttagcag aagagaagag catctctgct cgctatgccg aagagcggga
     6001 ccgggccgaa gccgaggcca gagagaaaga aaccaaagcc ctgtcactgg cccgggccct
     6061 cgaggaagcc ctggaggcca aggaggagtt tgagaggcag aacaagcagc tccgagcaga
     6121 catggaagac ctcatgagct ccaaagatga tgtgggaaaa aacgttcacg aacttgaaaa
     6181 atccaaacgg gccctagagc agcaggtgga ggaaatgagg acccagctgg aggagctgga
     6241 agacgaactc caggccacgg aagatgccaa gcttcgtctg gaggtcaaca tgcaggccat
     6301 gaaggcgcag ttcgagagag acctgcaaac cagggatgag cagaatgaag agaagaagcg
     6361 gctgctgatc aaacaggtgc gggagctcga ggcggagctg gaggatgaga ggaaacagcg
     6421 ggcgcttgct gtagcttcaa agaaaaagat ggagatagac ctgaaggacc tcgaagccca
     6481 aatcgaggct gcgaacaaag ctcgggatga ggtgattaag cagctccgca agctccaggc
     6541 tcagatgaag gattaccaac gtgaattaga agaagctcgt gcatccagag atgagatttt
     6601 tgctcaatcc aaagagagtg aaaagaaatt gaagagtctg gaagcagaaa tccttcaatt
     6661 gcaggaggaa cttgcctcat ctgagcgagc ccgccgacac gccgagcagg agagagatga
     6721 gctggcggac gagatcacca acagcgcctc tggcaagtcc gcgctgctgg atgagaagcg
     6781 gcgtctggaa gctcggatcg cacagctgga ggaggagctg gaagaggagc agagcaacat
     6841 ggagctgctc aacgaccgct tccgcaagac cactctacag gtggacacac tgaacgccga
     6901 gctagcagcc gagcgcagcg ccgcccagaa gagtgacaat gcacgccagc aactggagcg
     6961 gcagaacaag gagctgaagg ccaagctgca ggaactcgag ggtgctgtca agtctaagtt
     7021 caaggccacc atctcagccc tggaggccaa gattgggcag ctggaggagc agcttgagca
     7081 ggaagccaag gaacgagcag ccgccaacaa attagtccgt cgcactgaga agaagctgaa
     7141 agaaatcttc atgcaggttg aggatgagcg tcgacacgcg gaccagtata aagagcagat
     7201 ggagaaggcc aacgctcgga tgaagcagct taaacgccag ctggaggaag cagaagaaga
     7261 agcgacgcgt gccaacgcat ctcggcgtaa actccagcgg gaactggatg atgccaccga
     7321 ggccaacgag ggcctgagcc gcgaggtcag caccctgaag aaccggctga ggcggggtgg
     7381 ccccatcagc ttctcttcca gccgatctgg ccggcgccag ctgcaccttg aaggagcttc
     7441 cctggagctc tccgacgatg acacagaaag taagaccagt gatgtcaacg agacgcagcc
     7501 accccagtca gagtaaggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     7561 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7621 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7741 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     7801 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7861 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7921 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7981 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     8041 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     8101 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     8161 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     8221 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8341 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     8401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8701 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     8761 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8821 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8881 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8941 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9001 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9061 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9121 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9181 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9241 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9301 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9361 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9421 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9481 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9541 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9601 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9661 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9721 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     9781 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     9841 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     9901 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     9961 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
    10021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10861 tac
//
