LOCUS       sequence-251681-        6970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor vector for gamma and Delta TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6970)
  TITLE     Alcocer Lab plasmids
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMJA251
FEATURES             Location/Qualifiers
     source          1..6970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     546..565
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    564..619
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     665..684
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             1357..1368
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(1630..1833)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1659..1679)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1834..2137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2972..2989)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2978..3202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3248..3676
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3335..3354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3545..3566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3685..3705)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3690..4019
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3870..4005
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3932..3951
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4067..4114
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4133..4507
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4637..4758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4674..4693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4728..4747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4807..4823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4807..4823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4820..4842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4831..4847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4855..4861,4862..4879,4880..4885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4900..4921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5038..5055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5209..5797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5289..5308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5968..6759,6760..6828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6591..6610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6829..6933)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
       61 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
      121 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
      181 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      241 tgactagtaa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
      301 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
      361 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
      421 cagggggctg tttcatatac tgatgacctc tttatagcca cctttgttca tggcagccag
      481 catatggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
      541 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
      601 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
      661 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
      721 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattgaat tccttaataa
      781 acccaatgcc tttttcttat taatgcccaa ctttgtgacc caagaaaggt tggggttaga
      841 ttttcttttc catttgcagg aagaaaaccc gtttctttta aggcagacag caggtggata
      901 tggccaaaat aggcccccct cttgaggagc cggaggaggt acgtgtaata tgcagaggtg
      961 tttgtgagct gcagcattag tgtatcattt gcatcttttg accaattgtc tttggggtcc
     1021 attgtgatga catcatttgc atcttttgaa taactgtctt tggggtccac tgtggtgaca
     1081 tctgtcttta ttggaggaaa gataatttct tgatcaattc cgtttttatt attctcatgt
     1141 ctgacgatac atctgtgttc tttgtccagt gactcttctg gcaccgttaa ccagctaaat
     1201 ttcatgtatg tgtcgttagt cttcatggtg ttcccctcct gggagcccag aatcgtgttg
     1261 ctcttctttt cttgccaatg tatcttaata atatctggga aaaatttctc aagaagacaa
     1321 aggtatgttc cagccttctg gagttttgtt tcagcaattg aaggaagaaa aatagtgggc
     1381 ttgggggaaa catctgcatc aagttgttta tctgtgacaa caagtgttgt tccactgccg
     1441 agacgaaaga gtttcttata atacgcgtct cacatgcctt cctctctgag gccttggaag
     1501 gaaagggacc agatgaagag gagcagaaga tcctcttaaa actcaggccc cactcaacaa
     1561 ggaagtgatc cctagcacag aagacagtag gagggaggat ccctagcgga tctgacggtt
     1621 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     1681 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     1741 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     1801 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     1861 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     1921 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     1981 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2041 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2101 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     2161 aactaatgac cccgtaattg attactatta ataactactc gagtatgtcg aggtggcgtg
     2221 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggaggt
     2281 accaccggtc atatgccgcg ggagcccaga tcctgtgtgt gtgtgtgtgt gcttgtgcac
     2341 atctgttctc tgattggctg actgtacttc ctgagagtcc ctgtttaatg ttacaagaaa
     2401 cagctggaga cgatgcaggg ttctacccgg gtcatctttc gtctcggaaa aggaacccgt
     2461 gtgactgtgg aaccaagaag tcagcctcat accaaaccat ccgtttttgt catgaaaaat
     2521 ggaacaaatg tcgcttgtct ggtgaaggaa ttttacccca aggatataag aataaatctc
     2581 gtgtcatcca agaagataac agagtttgat cctgctattg tcatctctcc cggtgggaag
     2641 tacaatgctg tcaagcttgg taaatatgaa gattcaaatt cagtgacatg ttcagttcaa
     2701 cacgacaata aaactgtgca ctccactgac tttgaagtga agacagattc tacagatcac
     2761 gtaaaaccaa aggaaactga aaacacaaag caaccttcaa agagctgcca taaacccaaa
     2821 gccatagttc ataccgagaa ggtgaacatg atgtccctca cagtgcttgg gctacgaatg
     2881 ctgtttgcaa agactgttgc cgtcaatttt ctcttgactg ccaagttatt tttcttgtaa
     2941 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3001 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3061 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3121 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3181 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3241 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3301 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3541 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3601 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3661 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4021 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     4081 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     4141 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     4201 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     4261 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     4321 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     4381 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     4441 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     4501 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4561 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4621 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4741 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6961 cgtcgacgga
//
