LOCUS       sequence-251689-       11063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for 
            NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to 
            NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung 
            JK
  TITLE     CRISPR DNA base editors with reduced RNA off-target and self-editing
            activities.
  JOURNAL   Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6.
  PUBMED    31477922
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6."
COMMENT     Sequence Label: Target-AID (pRZ762)
FEATURES             Location/Qualifiers
     source          1..11063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1634..2347,2348..2350,2351..2353))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1637..2347,2348..2350,2351..2353))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1679..1700)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2028..2047
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2287..2308
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2354..2410)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2429..2680)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(2687..2707)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3341..3406)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(3614..3634)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3647..7720,7721..7723,7724..7750))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3647..7750)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7775..7795)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7798..7807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7873..7892)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7900..8916
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7954..7976)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8917..9194)
                     /label=chicken beta-actin promoter
     enhancer        9196..9575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9606..9710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9711..9779,9780..10571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9929..9948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct cagtggtatt
     1561 tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag
     1621 gagtgcggcc gcttcacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     1681 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     1741 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     1801 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     1861 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     1921 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     1981 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     2041 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     2101 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     2161 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     2221 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     2281 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     2341 ccttgctcac catcggtcca ggattctctt cgacatctcc ggcttgtttc agcagagaga
     2401 agtttgttgc gccgcttcca ccgcctccta gcatcttgat cttgttctct ccattagagt
     2461 cctgaatcac cagagcccag ggtttgtact ctggggcatc tgacgtgagc agcatcacgt
     2521 tctcatcggt gctctcatca tacgcagtgt gtacgagaat gtcactttcc ggcttattcc
     2581 caatgacctc ctcaacttct tcaggaagca tgagtatcga ctcttggatg accaactgtt
     2641 tgcctgtttc cttctctatg atgtcggaaa ggttggtcat ggtaccgact tttcttttct
     2701 tcttgggacc aacggctgga gacttagtgg tatgcagaat cttgacctgg atcatgatgc
     2761 tcagctcgga tctccacttc tccgcacgtt tcagtgtttt ctccaaccag cggttttcat
     2821 tgagctggtt atgagagctt tggatgaaaa tcttccgaca acactgatag tgctcggaaa
     2881 ccatcacgtt caggcctaca ccattgtccc tcagattcca gagtcctatc tggttccggg
     2941 cattcttctc gtagtacagc ttgcaggccc agattttcaa tgtgtgcccg tttccccgaa
     3001 gttcctgatt ataccattcc agaattttct cggcacaatc agcgcaagga ctccagctgg
     3061 aataccagtt gatcgtaaac tgcccaggat tatcccgaag atactcttcc acctttcgga
     3121 tactgaatat ctcagcgtgt attcctctct cggtgccact ttggggcttg ttgacggcat
     3181 agccccaaaa gcaagccctt ctttcgcccc ttcgcttcaa ctcaaacagc acgtagcagc
     3241 ggtgtgacac agatttcttg ttgttgaaga actgcttctt gaaggtgtaa atatccagtt
     3301 tctcatgaat gcgcacgtac tcggcgtctg tcatccctga tttatcgtcg tcgtctttgt
     3361 agtcgatgtc gtggtctttg taatccccat catggtcttt gtagtcaccg gaatatttct
     3421 ccacatatgg caccagaatc atgccccttt tcccttcgct atcctcggca ttccaccatt
     3481 gttcttcggg cttatctctg atgcggagaa tatcgccctt cttaaagggg agatcctcct
     3541 cgtcattccc attgaagtca aacagagctc gtacgtattc tgctgagcct ccaccgccgg
     3601 tacctcctcc acccaccttc ctcttcttct tggggtcagc cctgctgtct ccaccgagct
     3661 gagagaggtc gattcttgtt tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt
     3721 ccaggacctc ctttgtagag gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga
     3781 aggctgcagg cgcgcccaag ttggtcagag taaacaagtg gataatgttt tctgcctgct
     3841 ccctgatggg cttatccctg tgcttattgt aagcagaaag caccttatcg aggttagcgt
     3901 cggcgaggat cactcttttg gagaattcgc ttatttgctc gatgatctca tcaaggtagt
     3961 gtttgtgttg ttccacgaac agctgcttct gctcattatc ttcgggagac cctttgagct
     4021 tttcatagtg gctggccaga tacaagaaat taacgtattt agagggcagt gccagctcgt
     4081 tacctttctg cagctcgccc gcactagcga gcattcgttt ccggccgttt tcaagctcaa
     4141 agagagagta cttgggaagc ttaatgatga ggtctttttt gacctcttta tatcctttcg
     4201 cctcgagaaa gtcgatgggg tttttttcga agcttgatcg ctccatgatt gtgatgccca
     4261 gcagttcctt gacgcttttg agttttttag acttcccttt ctccactttg gccacaacca
     4321 gtacactgta agcgactgta ggagaatcga atccgccgta tttcttgggg tcccaatctt
     4381 ttttgcgtgc gatcagcttg tcgctgttcc ttttcgggag gatactttcc ttggagaagc
     4441 ctccggtctg tacttcggtc tttttaacga tgttcacctg cggcatggac aggaccttcc
     4501 ggactgtcgc gaaatcccta cccttgtccc acacgatttc tcctgtttct ccgtttgttt
     4561 cgataagtgg tcgcttccga atctctccat tggccagtgt aatctcggtc ttgaaaaaat
     4621 tcataatatt gctgtaaaag aagtacttag cggtggcctt gcctatttcc tgctcagact
     4681 ttgcgatcat tttcctaaca tcgtacactt tatagtctcc gtaaacaaat tcagattcaa
     4741 gcttgggata ttttttgata agtgcagtgc ctaccactgc attcaggtag gcatcatgcg
     4801 catggtggta attgttgatc tctctcacct tataaaactg aaagtccttt ctgaaatctg
     4861 agaccagctt agacttcaga gtaataactt tcacctctcg aatcagtttg tcattttcat
     4921 cgtacttggt gttcatgcgt gaatcgagaa tttgggccac gtgcttggtg atctggcgtg
     4981 tctcaacaag ctgccttttg atgaagccgg ctttatccaa ctcagacagg ccacctcgtt
     5041 cagccttagt cagattatcg aacttccgtt gtgtgatcag tttggcgttc agcagctgcc
     5101 gccaataatt tttcattttc ttgacaactt cttctgaggg gacgttatca ctcttccctc
     5161 tatttttatc ggatcttgtc aacactttat tatcaataga atcatctttg agaaaagact
     5221 ggggcacgat atgatccacg tcgtagtcgg agagccgatt gatgtccagt tcctgatcca
     5281 cgtacatgtc cctgccgttc tgcaggtagt acaggtagag cttctcattc tgaagctggg
     5341 tgttttcaac tgggtgttcc ttaaggattt gggaccccag ttcttttata ccctcttcaa
     5401 tcctcttcat cctttcccta ctgttcttct gtcccttctg ggtagtttgg ttctctcggg
     5461 ccatctcgat aacgatattc tcgggcttat gccttcccat tactttgacg agttcatcca
     5521 cgaccttaac ggtctgcagt attccctttt tgatagctgg gctacctgca agattagcga
     5581 tgtgctcgtg aagactgtcc ccctggccag aaacttgtgc tttctggatg tcctccttaa
     5641 aggtgagaga gtcatcatgg atcaactgca tgaagttccg gttggcaaat ccatcggact
     5701 taagaaaatc caggattgtc tttccactct gcttgtctcg gatcccattg atcagttttc
     5761 ttgacagccg cccccatcct gtatatcggc gcctcttgag ctgtttcatg actttgtcgt
     5821 cgaagagatg agcgtaagtt ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg
     5881 taagggtgag gacaatgtcc tcaagaatgt cctcgttctc ctcattgtcc aggaagtcct
     5941 tgtctttaat gattttcagg agatcgtgat acgttcccag ggatgcgttg aagcgatcct
     6001 ccactccgct gatttcaaca gagtcgaaac attcaatctt tttgaaatag tcttctttga
     6061 gctgtttcac ggtaactttc cggttcgtct tgaagaggag gtccacgata gctttcttct
     6121 gctctccaga caggaatgct ggctttctca tcccttctgt gacgtatttg accttggtga
     6181 gctcgttata aactgtgaag tactcgtaca gcagagagtg tttaggaagc accttttcgt
     6241 taggcagatt tttatcaaag ttagtcatcc tttcgatgaa ggactgggca gaggccccct
     6301 tatccacgac ttcctcgaag ttccagggag tgatggtctc ttctgatttg cgagtcatcc
     6361 acgcgaatct ggaatttccc cgggcgaggg ggcctacata gtagggtatc cgaaatgtga
     6421 ggattttctc aatcttttcc ctgttatctt tcaaaaaggg gtagaaatcc tcttgccgcc
     6481 tgaggatagc gtgcagttcg cccaggtgaa tctggtgggg gatgcttcca ttgtcgaaag
     6541 tgcgctgttt gcgcaacaga tcttctctgt taagctttac cagcagctcc tcggtgccgt
     6601 ccattttttc caagatgggc ttaataaatt tgtaaaattc ctcctggctt gctccgccgt
     6661 caatgtatcc ggcgtagcca tttttagact gatcgaagaa aatttccttg tacttctcag
     6721 gcagttgctg tctgacaagg gccttcagca aagtcaagtc ttggtggtgc tcatcatagc
     6781 gcttgatcat actagcgctc agcggagctt tggtgatctc cgtgttcact cgcagaatat
     6841 cactcagcag aatggcgtct gacaggttct ttgccgccaa aaaaaggtct gcgtactggt
     6901 cgccgatctg ggccagcaga ttgtcgagat catcatcgta ggtgtctttg ctcagttgaa
     6961 gcttggcatc ttcggccagg tcgaagttag atttaaagtt gggggtcagc ccgagtgaca
     7021 gggcgataag attaccaaac aggccgttct tcttctcccc agggagctgt gcgatgaggt
     7081 tttcgagccg ccgggatttg gacagcctag cgctcaggat tgctttggcg tcaactccgg
     7141 atgcgttgat cgggttctct tcgaaaagct gattgtaagt ctgaaccagt tggataaaga
     7201 gtttgtcgac atcgctgttg tctgggttca ggtccccctc gatgaggaag tgtccccgaa
     7261 atttgatcat atgcgccagc gcgagataga tcaaccgcaa gtcagcctta tcagtactgt
     7321 ctacaagctt cttcctcaga tgatatatgg ttgggtactt ttcatggtac gccacctcgt
     7381 ccacgatatt gccaaagatt gggtggcgct cgtgcttttt atcctcctcc accaaaaagg
     7441 actcctccag cctatggaag aaagagtcat ccaccttagc catctcatta ctaaagatct
     7501 cctgcaggta gcagatccga ttctttctgc gggtatatct gcgccgtgct gttcttttga
     7561 gccgcgtggc ttcggccgtc tccccggagt cgaacaggag ggcgccaatg aggttcttct
     7621 ttatgctgtg gcgatcggta ttgcccagaa ctttgaattt tttgctcggc accttgtact
     7681 cgtccgtaat gacggcccaa ccgacgctgt ttgtgccgat agcgagccca atggagtact
     7741 tcttgtccat tgccgcagga actccgtgga ttccgacttt acgcttcttc ttcggtgcca
     7801 tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt ctttgccaaa
     7861 atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc
     7921 atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc
     7981 cctgccccgt cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg
     8041 gagaagggga cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc
     8101 ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt
     8161 cggctccgca cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa
     8221 aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg
     8281 cccctccccc gagccctccc cggcccgagg cggccccgcc ccgcccggca cccccacctg
     8341 ccgccacccc ccgcccggca cggcgagccc cgcgccacgc cccgtacgga gccccgcacc
     8401 cgaagccggg ccgtgctcag caactcgggg aggggggtgc agggggggtt gcagcccgac
     8461 cgacgcgccc acaccccctg ctcacccccc cacgcacaca ccccgcacgc agcctttgtt
     8521 cccctcgcag ccccccccgc accgcggggc accgcccccg gccgcgctcc cctcgcgcac
     8581 actgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc
     8641 ggagccgcac gcggcgctcc ccacgcacac acacacgcac gcaccccccg agccgctccc
     8701 cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac
     8761 aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct
     8821 cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga
     8881 gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt
     8941 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
     9001 attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc
     9061 gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     9121 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     9181 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     9241 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     9301 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     9361 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     9421 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     9481 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     9541 tgattactat taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa
     9601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10861 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11041 aggcgcagcg gtcgggctga acg
//
