LOCUS       sequence-251696-       15736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating the full-length tetO array (from pLAU44) near the his-3 
            gene in N. crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15736)
  AUTHORS   Nguyen TS, Gladyshev E
  TITLE     Developing a tetO/TetR system in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2020 Mar;136:103316. doi: 
            10.1016/j.fgb.2019.103316. Epub 2019 Dec 9.
  PUBMED    31821884
REFERENCE   2  (bases 1 to 15736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2020-03"; volume: "136:103316. doi"; pages: " 
            10.1016/j.fgb.2019.103316. Epub 2019 Dec 9"
COMMENT     Sequence Label: pTSN6
FEATURES             Location/Qualifiers
     source          1..15736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2344..2362
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2637..2648
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4006..4024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4099..4117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4128..4146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4190..4208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4266..4284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4295..4313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4324..4342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4371..4389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4399..4417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4428..4446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4475..4493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4532..4550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4579..4597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4608..4626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4637..4655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4706..4724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4735..4753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4782..4800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4811..4829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4840..4858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4887..4905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4916..4934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4945..4963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5048..5066
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5095..5113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5151..5169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5198..5216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5227..5245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5256..5274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5303..5321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5332..5350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5361..5379
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5408..5426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5437..5455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5466..5484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5513..5531
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5542..5560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5572..5590
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5676..5694
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5723..5741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5752..5770
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5781..5799
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5828..5846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5886..5904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5933..5951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5962..5980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5991..6009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6037..6055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6066..6084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6095..6113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6142..6160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6200..6218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6248..6266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6305..6323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6352..6370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6394..6412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6470..6488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6546..6564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6575..6593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6604..6622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6651..6669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6680..6698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6709..6727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6756..6774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6785..6803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6814..6832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6861..6879
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6890..6908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6993..7011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7022..7040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7096..7114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7125..7143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7172..7190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7277..7295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7306..7324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7335..7353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7382..7400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7411..7429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7440..7458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7487..7505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7516..7534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7545..7563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7592..7610
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7620..7638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7649..7667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7725..7743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7754..7772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7801..7819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7830..7848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7859..7877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7905..7923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7934..7952
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7981..7999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8010..8028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(8080..8085,8086..8102,8103..8108)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             8297..8830
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    8848..8866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8877..8895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8983..9001
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9012..9030
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9059..9077
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9088..9106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9117..9135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9193..9211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9222..9240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9269..9287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9298..9316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9327..9345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9374..9392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9403..9421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9432..9450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9479..9497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9536..9554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9583..9601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9612..9630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9641..9659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9688..9706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9820..9838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9887..9905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9916..9934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9945..9963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9992..10010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10021..10039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10050..10068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10097..10115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10126..10144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10155..10173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10258..10276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10305..10323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10334..10352
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10409..10427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10438..10456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10467..10485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10514..10532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10543..10561
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10637..10655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10666..10684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10713..10731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10742..10760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10771..10789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10818..10836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10847..10865
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10876..10894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10923..10941
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10952..10970
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10981..10999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11057..11075
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11132..11150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11161..11179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11190..11208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11237..11255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11266..11284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11295..11313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11370..11388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11399..11417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11446..11464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11475..11493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11504..11522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11551..11569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11580..11598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11609..11627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11656..11674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11685..11703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11714..11732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11761..11779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11817..11835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11864..11882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11893..11911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11922..11940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11969..11987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11998..12016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12027..12045
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12074..12092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12103..12121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12132..12150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12179..12197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12208..12226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12237..12255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12284..12302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12399..12417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12475..12493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12504..12522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12533..12551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12580..12598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12609..12627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12638..12656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12685..12703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12714..12732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12790..12808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12819..12837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12848..12866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12895..12913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12924..12942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12953..12971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(13010..13026)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13011..13027)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(13056..13074)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(15735..15736,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(15736,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaataatacg actcactata gggagaccgg cagatccaat gcggatggat tcgctcactc
     2401 ggtgccgcgg cggtgcgaag ttcctgccgt ggattggggg attgcacaga cggtggagtg
     2461 cttgctacag ctaggtaccc agttgcacac caaacggtgc ccctgtgccc ctgcgtctag
     2521 gtgtgtcctg tgagcaactg cgcccgcgcg cggcaccggg aagctgcggg aacctcaatg
     2581 gatgattggc gccagatccc cggcatcacc tcagcacatg ggcagcccga gaaggaatcg
     2641 agggtagaag atattccagg gtttccgcta gcacaacttc caagcgcctc taccgctact
     2701 ccacacaccc acgctgaatg gcacaaccac tgtaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaagagccca ttctcacaag gagcaacact gtgctgctat
     2941 catagtacac tacaagcaaa gtcgggatgt tgctagaatc cgtcggctac gtcggtagat
     3001 acctcccata ggcagtaagt ctaggtatcc aatttatagt tccgcgtatt tcttcgtcga
     3061 acggggcaca gcaaagccta acagccgcag tgcctcgcaa ccaacaccaa gcagtaatct
     3121 catccatcca tacagatcct cccacaatat ctttacatgc gcacttgcgc tgtgttagca
     3181 gtttaggttc cactaccaag gctgacacga gcagcactga cagcaaactg ctgcttgggg
     3241 ccaagaaatt ggcagtttgc tgagcaccta catatcagta cacatcaacc ctggtttaat
     3301 tgttagcctg gtactgttaa ccgtcatgaa cgaggacgtg gaggaagaac cacgctaaca
     3361 attgaaccag ggttgatgta tacctctctt accatatttg cttgcttcca cgtccctatc
     3421 ctcaagcgat tttgttcttg actttttgta tttcaacgca aaggccattc aggtttctca
     3481 tgttcaaccc tttggatggc gatgccgttc gtcccaggac ctcgtgacct ttgaaagacg
     3541 agaaatatat cccaaagcaa cttctgctta tggacaagca actgcatggc ggcccacaac
     3601 tcatatctcg cgtaatcatc caaacgctag gcccgagtac tctactgttc tcgagacacc
     3661 taccataata tgtacccaat cagacatgac aacagtcaac caaacatacc ctgatccggc
     3721 caaaccctga catccctaga tgatggttag gcacacaaat ggaaaacggg actcgttggt
     3781 taggtgggaa cgcttgtatt gcagccagcc aaccgactct cctcgtccct ctcgagtccc
     3841 gttattgccg tttgacccag ccgcccagcc gccaaacagc tgagggagcc aatggtctaa
     3901 tacaccgtcc aacggcccca gtgatgtttc aaacttgatt gacagcgaac gaaacccctg
     3961 aaacccgtta tagatatgta tagatttgac ggggtagctt ggccctaata cgactcacta
     4021 tagggcgaat tggagctcca ccgcggtggc ggccgcctct agcagggagg tcctatcagt
     4081 gatagagaag acgaaccgtc cctatcagtg atagagaggg gaaaggatcc ctatcagtga
     4141 tagagacgaa gtgggactct agcacggagg cgggtcccta tcttgcgcgt ccctatcagt
     4201 gatagagaag tggggaggtc cctatcagtg ataaagacag tgtgtggctc tagcagcggg
     4261 cagggtccct atcagtgata gagagaacag gagatcccta tcagtgatag agacgacgga
     4321 aggtccctat cagtgataga gaaaggtaat cgctctagca agcgaggggg tccctatcag
     4381 tgatagagag acaggtggtc cctatcagtg atagagaaag gagacggtcc ctatcagtga
     4441 tagagaatgg ggccagctct agcagaaggg aaggtcccta tcagtgatag agagacctat
     4501 agatcctatc agtgatagag aacaggatgg atccctatca gtgatagaga agggacgaag
     4561 ctctagcagg ggagacggtc cctatcagtg atagagaggc gcgcttgtcc ctatcagtga
     4621 tagagagacg ccggagtccc tatcagtgat agagagaagg gacaactcta gcaatggtcc
     4681 ctacagtgat agagaacaga acgcatccct atcagtgata gagactgcgg actatcccta
     4741 tcagtgatag agacggggtg catctctagc agggccgaga atccctatca gtgatagaga
     4801 atgcggggcc tccctatcag tgatagagag ccgaaatgtt ccctatcagt gatagagaag
     4861 cgagctggct ctagcagagg gcacaatccc tatcagtgat agagagacgg gaccgtccct
     4921 atcagtgata gagataaagc cgagtcccta tcagtgatag agatggagag agactctagc
     4981 agaaagagtt gtccctatca gtgacagaga aagtcagagg tccctcagtg atagagaggg
     5041 ggagatgtcc ctatcagtga tagagacaga tcagcactct agcatgaagt gtagtcccta
     5101 tcagtgatag agacactcag gaatccctca gtgatagaga atctcgggtg tccctatcag
     5161 tgatagagag ggggctactc tctagcaggg tctctagtcc ctatcagtga tagagagagc
     5221 gcagactccc tatcagtgat agagacgaag agccatccct atcagtgata gagacaaggg
     5281 ataactctag caatggggac catccctatc agtgatagag atggcggcgg ttccctatca
     5341 gtgatagaga cgcaggcgga tccctatcag tgatagagaa gtaaggaggc tctagcatgg
     5401 agtctgatcc ctatcagtga tagagaccgg gagacatccc tatcagtgat agagagcggt
     5461 ttaaatccct atcagtgata gagaaccgcg gggactctag cacggagccg tgtccctatc
     5521 agtgatagag aagagaggag gtccctatca gtgatagaga cgtggagaag gtccctatca
     5581 gtgatagaga gaatggtagg ctctagcaga ggccgctatc cctatagtga tagagaatta
     5641 tacacgtccc tatcagtgat agggaggaag atgtatccct atcagtgata gagagtggaa
     5701 ggtactctag cagagagagg cgtccctatc agtgatagag aggaagtaag ctccctatca
     5761 gtgatagaga gagtgctaag tccctatcag tgatagagaa agagacggac tctagcatga
     5821 gagcgactcc ctatcagtga tagagacgcg gagtgatccc tattagtgat agagacgagg
     5881 ggggatccct atcagtgata gagagcctta taatctctag caggggagct aatccctatc
     5941 agtgatagag agtcatataa atccctatca gtgatagaga gcgagccgac tccctatcag
     6001 tgatagagac cgttaaaaac tctagcaggt gaggtatccc tatcagtgat agagaacaac
     6061 gaaaatccct atcagtgata gagagggaaa aaagtcccta tcagtgatag agaggcgggg
     6121 agactctagc attgcaacta gtccctatca gtgatagaga gagacaaacg tccctaccag
     6181 tgatagagat tccggagagt ccctatcagt gatagagacg aaaaagagtc tctagcacgg
     6241 ggggatgtcc ctatcagtga tagagagagg cctgagtcct atcagtgata gagaaagcac
     6301 agggtcccta tcagtgatag agagggtaga gcgctctagc aggtgccaac gtccctatca
     6361 gtgatagaga gcgcggcagg tccctatcag gaatccctat cagtgataga gagggcgggg
     6421 agctctagca gggccaaacg tccctctcag tgatagagaa gggccaaaat ccctatcagt
     6481 gatagagata taaaagcgtc cctatcagtg gtagagagga agcagcactc tagcagcata
     6541 aatgatccct atcagtgata gagagagagg cggctcccta tcagtgatag agaaaagggc
     6601 cggtccctat cagtgataga gaggtaacgc atctctagca agccaggggg tccctatcag
     6661 tgatagagag ggcgatgcct ccctatcagt gatagagagc gccgggggtc cctatcagtg
     6721 atagagaaga atagtagctc tagcagaagg aataatccct atcagtgata gagaaggcaa
     6781 tggctcccta tcagtgatag agaagcacgc atatccctat cagtgataga gaggcgcggg
     6841 cactctagca acggcggaga tccctatcag tgatagagag gagaccacat ccctatcagt
     6901 gatagagaat gagggggatc cctatagtga tagagaggag ggcagcctct agcagtatgg
     6961 acgatcccta tcaggataga gaaaggggga actccctatc agtgatagag agtaaggtcg
     7021 ttccctatca gtgatagaga gggcctggga ctctagcagt ggtagctgtc cctaagtgat
     7081 agagaaacca gaatatccct atcagtgata gagaaagaga tggttcccta tcagtgatag
     7141 agaaagggcc acactctagc aaagtagcag ttccctatca gtgatagaga aagttgttga
     7201 tccctatctg tggtagagag agcagaccac ccctatcagt gatagagagg gaagggccct
     7261 ctagcagacg ggggaatccc tatcagtgat agagataaac gctcttccct atcagtgata
     7321 gagacctagc agggtcccta tcagtgatag agacccccta gtgctctagc aacgaaacaa
     7381 atccctatca gtgatagaga aggagtggta tccctatcag tgatagagag caggggaggt
     7441 ccctatcagt gatagagaaa acgcatcact ctagcacggc cgcgtgtccc tatcagtgat
     7501 agagaagcgg cggaatccct atcagtgata gagacgacgt gcagtcccta tcagtgatag
     7561 agaaaagaag ccactctagc aggcatgggg gtccctatca gtgatagaga gagatagcgt
     7621 ccctatcagt gatagagacg gcgttccgtc cctatcagtg atagagaaaa ccgggcactc
     7681 tagcaagaga gcgagaccct atcagtgata gagatcggga cgtatcccta tcagtgatag
     7741 agatgagggg gactccctat cagtgataga gataaatata gactctagca aggcatgaca
     7801 tccctatcag tgatagagat ggaaaaactt ccctatcagt gatagagaaa gggacaattc
     7861 cctatcagtg atagagaggt actggcactc tagcacgcgg gtgctcccta tcagtgatag
     7921 agaacgcagc aggtccctat cagtgataga gaagatgggg gcctctagca aaaaatgcgg
     7981 tccctatcag tgatagagag acaagaagtt ccctatcagt gatagagaca ggggcacttc
     8041 cctatcgtga tagagatgag agactactct agcacccagt tgacataagc ctgttcggtt
     8101 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     8161 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
     8221 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     8281 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
     8341 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
     8401 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
     8461 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
     8521 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
     8581 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
     8641 cgagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
     8701 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
     8761 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     8821 cgccacctaa tctagcactg gcggcggtcc ctatcagtga tagagatgga gttggatccc
     8881 tatcagtgat agagactaaa gggggtccct atcagtgatg gagaaggtaa tacgctctag
     8941 caaagacgag agtccctatc agtgatagag ggaggtgtgg aatccctatc agtgatagag
     9001 agtaaaacgg atccctatca gtgatagaga acgcagtaga ctctagcagg ctttgaagtc
     9061 cctatcagtg atagagaaat ccagtggtcc ctatcagtga tagagaagga agtagttccc
     9121 tatcagtgat agagaatggc agaggctcta gcagagacgg caatccccat cagtgataga
     9181 gaggatcaga attccctatc agtgatagag aaatatggca atccctatca gtgatagaga
     9241 tcatagcggc ctctagcaga gttgggggtc cctatcagtg atagagagta tgtagtatcc
     9301 ctatcagtga tagagataga gaggaatccc tatcagtgat agagagggag aagagctcta
     9361 gcaagggggt acgtccctat cagtgataga gataggggga ggtccctatc agtgatagag
     9421 agcgagggga atccctatca gtgatagaga aggggactca ctctagcaac gatacaggtc
     9481 cctatcagtg atagagagga tagccagtcc ctatcagtat agagaactgg gcaaatccct
     9541 atcagtgata gagaaaatgg catgctctag cagaaaatca agtccctatc agtgatagag
     9601 aggtgaggag ctccctatca gtgatagaga gggttatcag tccctatcag tgatagagaa
     9661 aaatagagtc tctagcaggg taagtcgtcc ctatcagtga tagagaagaa taagtatccc
     9721 tatcagtgat agagccaaag catgatccct atagtgatag agatggattc aactctagca
     9781 ccggaaaagt tccctatcag tggtagagaa gctgaaaagt ccctatcagt gatagagagg
     9841 cgaatgactc cctatcagtg aggagcggct ctagcaaaga gaggagtccc tatcagtgat
     9901 agagatcaga ggcactccct atcagtgata gagaggggga tacatcccta tcagtgatag
     9961 agaaagcgtt ggactctagc agcgaagcga ttccctatca gtgatagaga aaggagagga
    10021 tccctatcag tgatagagag tggggcgcat ccctatcagt gatagagacg aggtccagct
    10081 ctagcaagca ggtccatccc tatcagtgat agagagaagg ggaggtccct atcagtgata
    10141 gagaaaagga tggatcccta tcagtgatag agagcgatcg gaactctagc agactaagag
    10201 ttcctatcag tgatagagac aagaaagcat ccctaacagt gatagagaga gcgcaagtcc
    10261 ctatcagtga tagagagaca gggcagctct agcacgcgta aggatcccta tcagtgatag
    10321 agatcggtga atatccctat cagtgataga gacgggaggg cctcctatca gtgatagaga
    10381 tacatagagg ctctagcaaa agcgaacctc cctatcagtg atagagaggt gcgtgaatcc
    10441 ctatcagtga tagagaaagc cagcgttccc tatcagtgat agagaggcaa acatgctcta
    10501 gcagaaagtt tactccctat cagtgataga gaagaaagga cttccctatc agtgatagag
    10561 agaaaagcga ctcagtgata gagaaggacg aaatctctag cagtcaatgg gctcccttga
    10621 tagagaaaat acgggttccc tatcagtgat agagaggaga ggggctccct atcagtgata
    10681 gagagcacgc aggactctag cacgtaaagt gctccctatc agtgatagag aagcgggaaa
    10741 atccctatca gtgatagaga gctacagagc tccctatcag tgatagagat ctcagagggc
    10801 tctagcaaac gggagagtcc ctatcagtga tagagagagt taagactccc tatcagtgat
    10861 agagacgcat cacggtccct atcagtgata gagacacgtt tgaactctag cagaagtgcg
    10921 gatccctatc agtgatagag actaaagaat ttccctatca gtgatagaga acattcacgt
    10981 tccctatcag tgatagagag tgtgggcgcc tctagcatcg aggggaatcc ctatgagtga
    11041 tagagacgga gataagtccc tatcagtgat agagacgata tgagtcccta tcagtggtag
    11101 agaggggaca tcgctctagc agtgggatgg atccctatca gtgatagaga acacaggcca
    11161 tccctatcag tgatagagag gcgtaacact ccctatcagt gatagagata cagacaatct
    11221 ctagcaagca tgaggctccc tatcagtgat agagagcgga cgggctccct atcagtgata
    11281 gagagtaggg gccatcccta tcagtgatag agagaaaaag gaactctagc actcgggatg
    11341 gtccttatca gtgatagaga gaatataggt ccctatcagt gatagagagc cgtcacgctc
    11401 cctatcagtg atagagagag ggggtgcctc tagcactccg cgggctccct atcagtgata
    11461 gagaggcaag gaagtcccta tcagtgatag agaaatcgat cgttccctat cagtgataga
    11521 gatgggtcta aactctagca tagcagaagc tccctatcag tgatagagag gggggagatt
    11581 ccctatcagt gatagagaga caggaaaatc cctatcagtg atagagacgc tacagcactc
    11641 tagcacaaac cctggtccct atcagtgata gagagggatc gtaatcccta tcagtgatag
    11701 agaagagcat ggatccctat cagtgataga gagcgcgcag ggctctagca cgtcatggta
    11761 tccctatcag tgatagagag cacgcagacc ctatcagtga tagagagagg caggggtccc
    11821 tatcagtgat agagacaggt cccgactcta gcatcgtgac aagtccctat cagtgataga
    11881 gaaccccgag cctccctatc agtgatagag agaatcagag gtccctatca gtgatagaga
    11941 aacacgggtc ctctagcaag aagatcaatc cctatcagtg atagagaaaa ggtgagatcc
    12001 ctatcagtga tagagactca gcgctgtccc tatcagtgat agagaatgca gggagctcta
    12061 gcagaatgac aggtccctat cagtgataga gacccaaggt cgtccctatc agtgatagag
    12121 agctgtgcat ctccctatca gtgatagaga ccgcaagaag ctctagcaga agcagttgtc
    12181 cctatcagtg atagagaagc ggaaccatcc ctatcagtga tagagaagaa tagaagtccc
    12241 tatcagtgat agagagatat accaactcta gcaaaggccg ggatccctat cagtgataga
    12301 gacagcacgg agaatgcagc gcctccctat cagtgaaagg agaagaagtg gctctagcaa
    12361 ggcccgggtt ccctatcagt tatagagaag gtgagcggtc cctatcagtg atagagagaa
    12421 cacgaaatcc ctatctgtga tagagagcaa gggggtctct agcagcggag gcagtcccta
    12481 tcagtgatag agactgggga cgatccctat cagtgataga gagagtcgcg gttccctatc
    12541 agtgatagag aaacgcaggt gctctagcaa tgcatattat ccctatcagt gatagagaaa
    12601 ggcgaggctc cctatcagtg atagagatga cgaaagatcc ctatcagtga tagagacgga
    12661 gccaaactct agcagtgggt ggggtcccta tcagtgatag agagagggaa gggtccctat
    12721 cagtgataga gaggaagaga ggtccctgtc agtgatagag aggtgagacc actctagcaa
    12781 gagtggggat ccctatcagt gatagagaac gttgatcatc cctatcagtg atagagaaga
    12841 gtgaaagtcc ctatcagtga tagagaagca ccgcccctct agcacggccg gccatcccta
    12901 tcagtgatag agagggcgac gtgtccctat cagtgataga gaaatctagc aatccctatc
    12961 agtgatagag agacggacgt actctagacg aattcgatat caagcttatc gataccgtcg
    13021 acctcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattagggcg
    13081 tggacggcta atggggtctg aatgctaaaa gacaccattt cccacactcc ctctgatttt
    13141 ttttatggtt tttttttatc ttcatttttc gccgtctttt attcggttgt ttgggaggtt
    13201 cacatgcaac gtgcaagact gcaagttttt tgagatgggc gcttcatgca atgcagcatc
    13261 actgcaggga cattgtgaca tccacgtttc gatcgtctgt gcttcccccg ttcatgtacg
    13321 catgatctga aaaaaaaaaa aaaaaaaaag gttgatgtaa taatgaaaat aaacacccag
    13381 ggacgagcac ctcatctgct ggaataggga gaaaaactca agggccgttt gggtctccac
    13441 acttaccaac agattcatgt actgatcttc agcctgtaca gaacagtaca atgtccaaaa
    13501 ctcgttatca ttactctatg tgcccaaaat gacccagata tacatcaacc cactacatcc
    13561 ttttgttctt gttttttttt tttttttttg ctacttcgaa aagaatttcc gtttagttgc
    13621 tcttgctcat gtgctcaaga cggatgctga ccgcccttct gtgagcctcg agctcctcaa
    13681 ccttagccaa ctgcatgaca gcctggccga cgtttttgag accctcggca gtcaagttgg
    13741 agctggtaat gtgcttgacg aacgaggcga aattgacgcc agagtactgc ttgccaaagc
    13801 cataggtagc tatgtgtctt gacgttagca gaagaatctg tgaagcgggg ctatttatga
    13861 tatgtaactt acgcagcgag tggttaacac cagcagagta atcgccaacg gactcaggag
    13921 tccaggcgcc aatgaagaca ctaccggcgt tcatgacaag atcgacagcc ttctcggcct
    13981 ccttgatctg gaggatcaag tgctcaggag cgtacttgtt gctgagctcc atggcctcct
    14041 cgacggtctt gacctgcacg gtgatcgagt gggcgatgga gccacggaca atctggacgc
    14101 gaggaagctc cgtagcctga cggtgaacct cgtcctcaat agcctgaaga tgctcctcgt
    14161 cgaggtcaat agcgatcagg atgacctgac tgtcaacgcc gtgctcagcc tgggacagga
    14221 gatccgaggc aacgaacgcg gggttggcgt ccttgtcagc gatgaccagc acctcggacg
    14281 ggccagcggg catgtcaata ccaacggcag cgttggtgtc gttgctgacg aacatcttgg
    14341 cagcagtgac gaactggtta ccggggccga gaatcttgtc gaccttggtg atgctctcgg
    14401 tgccgtaggc catggcagct acggcctggg caccgccggc aagcacgatg gactcggccc
    14461 caaccttgtg agcgacgtgg acaatctcgg gagtgatggt tccgtcggcg cggggaggag
    14521 aggcgaacac aatcttgttg cagccggcga ccatggcggg aacacccagc ataagggcag
    14581 tgctggggag aacggcggta ccgccgggga tgtagcagcc gacggcctcg atgggacgag
    14641 agaaacggct gcagacaaca ccgggcatgg tctcgacctg gaggggcttc tcctccttct
    14701 gggcggcgtg gaacttgcgg atgttctcga aggacacgtc gatggcagca atggtctcct
    14761 cagggagctg cataagctcc ttggggaagg gcgccttcag gacggggcta gtaagagagg
    14821 tagccttctc gaacttgtga gtgtacgaca gaacagcctt gtcgccgttc ttgcggacgt
    14881 cctcgatgat ggggacaatg atcttgtaga tggcatcgga cgacttttgc gcaggacgct
    14941 tgagagcagc atcgagctcc tcggtagaga ccttggaggc gtcgaaacgt ctcatggtga
    15001 tcttctcagg ggtggtctcc tgggcggcct ccttggtgac aggggccgaa gtggcagcga
    15061 gagcggacgc cgcaggcttg atgccctcct tctcagccca cttaccctta gcatctccag
    15121 tcctgcgctt gaccttccag ctcttggcgt caaggctcct ttcgatatcg gcaagagtaa
    15181 cgccggcagc aacggccctg gtaagagcaa agtagaagag atcggcagcc tcaaaggcga
    15241 tttcctgggg ggtctgagcg gtgcagagct cctcagcctc ctccatgatc ttggcccgga
    15301 ctagcttctc atcggagaag agacgggcag tgtaggagcc ctcgggggca gactgtttcc
    15361 tcgaaatcaa agtctgctcg agcttgggaa ggcctttgag ctgaccaaag cagccggact
    15421 gatcgaggtg gcagaaacga cccttctgct tcacgacaaa cttgagagca tcgttatcgc
    15481 agtcaagcga gatgcggacg agctcctgag tgtctccgga agtagcaccc ttgtaccaga
    15541 gaccgcgctt ccggctctga tagacaccag tctgtgtcct gagggcctcg ttcacactct
    15601 cggcactgct gtagaccaga cccagagcag tgtcgtgctc atcaacgaca acggtgggga
    15661 gaagaccatc gggacggtca gacttccaga cgctcgagag gatggtggag atggcaagct
    15721 tcagctgctc gagttc
//
