LOCUS       sequence-251705-        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a fluorecent protein with an RNA binding peptide: 
            YPet-PCP.Expression with constitutive E. coli RNAP promoter 
            (J23106), ribozyme RiboJ and RBS BBa_B0034..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, 
            Simmel FC
  TITLE     Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in
            a Cell-Free Gene Expression System.
  JOURNAL   Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 
            10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
  PUBMED    29564885
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.8b00526"; journalName: "Nano Lett"; date: 
            "2018-04-11- 11"; volume: "18"; issue: "4"; pages: "2650-2657"
COMMENT     Sequence Label: pSB1C3_YPet-PCP (y-plasmid)
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        169..203
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             292..303
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    1487..1507
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1508..1565
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1589..1606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1760..2348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1840..1859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2531..2625)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2646..3305)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3220..3239
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3467..3485
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgagtaagc
       61 ccctctagag gaccacgcat cgtgatgcct atgcgcggta gtcccacctt gtccactaga
      121 atggaagatt ggcacgtatc aagactttgg agtagtacca taacgccgtt tacggctagc
      181 tcagtcctag gtatagtgct agctagcgca gcgctcaacg ggtgtgcttc ccgttctgat
      241 gagtccgtga ggacgaaagc gcctctacaa ataattttgt ttaatcatga gaaagaggag
      301 aaaactagat gtctaaaggt gaagaactgt ttacgggtgt cgtgccgatt ctggtcgagt
      361 tggacggcga cgtgaacggt cacaaattca gcgtgagcgg cgagggcgag ggtgacgcga
      421 cgtacggtaa gctgactctg aagctgctgt gcaccacggg taaattgccg gttccgtggc
      481 cgaccctggt cacgacgctg ggttatggtg tacaatgttt tgcacgctat ccggaccaca
      541 tgaaacagca cgatttcttc aagagcgcga tgccggaagg ctatgttcag gaacgtacca
      601 tctttttcaa agatgatggt aattacaaaa cccgcgcaga agtgaagttc gagggtgaca
      661 ccctggtgaa ccgtattgag ctgaagggta ttgacttcaa ggaagatggc aatattctgg
      721 gtcacaaact ggagtacaac tataacagcc ataacgtcta catcaccgcg gataagcaaa
      781 aaaatggtat caaagcaaat ttcaagattc gccacaacat cgaagatggc ggcgtgcaac
      841 tggccgatca ttatcagcag aataccccaa tcggtgacgg tccggtgctg ttgccggata
      901 accactacct gagctatcaa agcgcgttgt tcaaagaccc gaatgaaaaa cgtgaccaca
      961 tggttctgct ggaatttctg accgctgcgg gcatcactga aggcatgaat gaactgtaca
     1021 agacgcgtgg tggcggcggt tcgatgagca agactatcgt tttgtccgtc ggcgaggcta
     1081 cccgtacctt gaccgaaatt caatccaccg cggaccgtca aatttttgag gaaaaagtcg
     1141 gtcctctggt gggtcgtctg cgtctgaccg cgagcctgcg ccagaacggt gccaaaacgg
     1201 cataccgtgt taatctgaaa ctggatcagg ccgacgttgt ggacagcggt ctgccgaaag
     1261 tccgctacac ccaggtgtgg agccacgatg tgacgatcgt tgcgaatagc accgaagcga
     1321 gccgcaagag cctgtacgac ctgaccaaga gcctggtggc aacgtcccaa gttgaagatc
     1381 tggttgttaa cctggtgccg ctgggtcgtt aaagcatgcc ggaggaaaca cagaaaaaag
     1441 cccgcacctg acagtgcggg cttttttttt cgaccaaagg ggtgcatact agtagcggcc
     1501 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     1561 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1741 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     2521 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     2581 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     2641 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2701 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2761 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2821 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2881 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2941 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3001 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3061 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     3121 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3181 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3241 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3301 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3361 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     3421 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3481 gaggcaga
//
