LOCUS       sequence-251712-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Lin PH, Shenoy S, Galitski T, Shalloway D
  TITLE     Transformation of mouse cells by wild-type mouse c-Src.
  JOURNAL   Oncogene. 1995 Jan 19. 10(2):401-5.
  PUBMED    7530829
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            1995 Jan 19. 10(2):401-5."
COMMENT     Sequence Label: pM5MF529
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     complement(981..1000)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(5995..6583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6075..6094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6754..7545,7546..7614))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7377..7396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7615..7719)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7787..7805
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcaggcgca
      901 gaactggtag gtatggaaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      961 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     1021 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     1081 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     1141 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaca
     1201 gcacacaagg tagtttcacc aaaccaaaag cgctaccacc cgcaattttc ctcatttgtg
     1261 gggatcgcgc atggcaagga attcccagtc gtccggtagg gggcccctgc tatttaggca
     1321 agcttaccat gttagcacct aagcatacag atattctcaa ggtgcttgtc aattcatcgc
     1381 ggacaggtat aagacgtaaa cgaagcacct cacacctgga tgatacatgc tcagatgaag
     1441 tgcagctttg gggtcctaca gcaagaatct ttgcatctat cctagccccg ggggtagcag
     1501 ctgcgcaagc cttaagagaa attgagagac tagcctgttg gtccgttaaa caggctaact
     1561 tgacaacatc actcctcggg gacttattgg atgatgtcac gagtattcga cacgcggtcc
     1621 tgcagaaccg agcggctatt gacttcttgc tcctagctca cggccatggc tgtgaggacg
     1681 ttgccggaat gtgctgtttc aatttgagtg atcacagtga gtctatacag aagaagttcc
     1741 agctaatgaa ggaacatgtc aataagatcg gcgtggacag cgacccaatt ggaagttggc
     1801 tgcgaggact attcggggga ataggagaat gggccgttca tttgctgaaa ggactgcttt
     1861 tggggcttgt agttattttg ttgctagtag tgtgcctgcc ttgccttttg caaatcgtat
     1921 gcggtaacat cagaaagatg attaataact ccatcagcta ccacacggaa tataagaagc
     1981 tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata aggcagtaca tgggtggtgg
     2041 tatagcgctt gcgagtcggg ttgtaacggg acatggctta actaagggga ctatggcatg
     2101 tataggcgca aagcggggtt tcggttgtac gcgattagga gtccccttag gatatagtag
     2161 acacgctttt gcatatgata cataacttcc ctgttttgcc cttagactat tcaagttgcc
     2221 tctgtggatt agggctggag gcagctcgga tggtctgacg gccagataag gcaggaaaga
     2281 cagctattgg taattgtgaa atacgctttt gtctgtgtgc tgcaggagct gagctgactc
     2341 tgctggtggc ctcgcgtacc actgtggcca ggcggtagct gggacgtgca gccgaccacc
     2401 atgggcagca acaagagcaa gcccaaggac gccagccagc ggcgccgcag cctggagccc
     2461 tcggaaaacg tgcacggggc agggggcgcc ttcccggcct cacagacacc gagcaagccc
     2521 gcctccgccg acggccaccg cgggcccagc gccgccttcg tgccgcccgc ggccgagccc
     2581 aagctcttcg gaggcttcaa ctcctcggac accgtcacct ccccgcagag ggcggggcct
     2641 ctggcaggtg gggtgaccac ctttgtggcc ctctatgact atgagtcacg gacagagact
     2701 gacctgtcct tcaagaaagg ggagcggctg cagattgtta acaacacgga gggagactgg
     2761 tggctggcac actcgctgag cacgggacag accggttaca tccccagcaa ctatgtggcg
     2821 ccctccgact ccatccaggc tgaggagtgg tactttggca agatcactag acgggaatca
     2881 gagcggctgc tgctcaacgc cgagaacccg agagggacct tcctcgtgag ggagagtgag
     2941 accacaaaag gtgcctactg cctctctgta tccgacttcg acaatgccaa gggcctaaat
     3001 gtgaaacact acaagatccg caagctggac agcggcggtt tctacatcac ctcccgcacc
     3061 cagttcaaca gcctgcagca gctcgtggct tactactcca aacatgctga tggcctgtgt
     3121 caccgcctca ctaccgtatg tcccacatcc aagcctcaga cccagggatt ggccaaggat
     3181 gcgtgggaga tcccccggga gtccctgcgg ctggaggtca agctgggcca gggttgcttc
     3241 ggagaggtgt ggatggggac ctggaacggc accacgaggg ttgccatcaa aactctgaag
     3301 ccaggcacca tgtccccaga ggccttcctg caggaggccc aagtcatgaa gaaactgagg
     3361 cacgagaaac tggtgcagct gtatgctgtg gtgtcggaag aacccattta cattgtgaca
     3421 gagtacatga acaaggggag tctgctggac tttctcaagg gggaaacggg caaatatttg
     3481 cggctacccc agctggtgga catgtctgct cagatcgctt caggcatggc ctatgtggag
     3541 cggatgaact atgtgcaccg ggaccttcga gccgccaata tcctagtagg ggagaacctg
     3601 gtgtgcaaag tggccgactt tgggttggcc cggctcatag aagacaacga atacacagcc
     3661 cggcaaggtg ccaaattccc catcaagtgg acagcccccg aggcagccct ctatggccgg
     3721 ttcaccatca agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag
     3781 ggccgggtgc catacccagg tgagagttag catcaacccc acaccccgtg ggttgggcac
     3841 agcgtgtgac ccggccccgc tgcccttctt tccacaggga tggtcaacag ggaggtgctg
     3901 gaccaggtgg agaggggcta ccgcatgccc tgcccgcccg agtgccccga gtcgctgcat
     3961 gacctcatgt gccagtgctg gcggaaggac cctgaggagc ggcccacttt tgagtacctg
     4021 caggccttcc tggaggacta cttcacctcg acagagcccc agttccagcc tggagagaac
     4081 ctatagatct ctagaagctt atcgattagt ccaatttgtt aaagacagga tatcagtggt
     4141 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     4201 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     4261 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     4321 gagaatagaa aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     4381 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     4441 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     4501 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     4561 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     4621 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     4681 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     4741 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     4801 gtatttacat taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg
     4861 gagtattcag aatgtgtcat aaatatttct aattttaaga tagtatctcc attggctttc
     4921 tactttttct tttatttttt tttgtcctct gtcttccatt tgttgttgtt gttgtttgtt
     4981 tgtttgtttg ttggttggtt ggttaatttt tttttaaaga tcctacacta tagttcaagc
     5041 tagactatta gctactctgt aacccagggt gaccttgaag tcatgggtag cctgctgttt
     5101 tagccttccc acatctaaga ttacaggtat gagctatcat ttttggtata ttgattgatt
     5161 gattgattga tgtgtgtgtg tgtgattgtg tttgtgtgtg tgattgtgta tatgtgtgta
     5221 tggttgtgtg tgattgtgtg tatgtatgtt tgtgtgtgat tgtgtgtgtg tgattgtgca
     5281 tgtgtgtgtg tgtgattgtg tttatgtgta tgattgtgtg tgtgtgtgtg tgtgtgtgtg
     5341 tgtgtgtgtg tgtgtgtgtg ttgtgtatat atatttatgg tagtgagagg caacgctccg
     5401 gctcaggtgt caggttggtt tttgagacag agtctttcac ttagcttgga attctcatgt
     5461 ttgacagctt atcatcgatg ataagctgtc aaacatgaga atttcgaccg atgcccttga
     5521 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     5581 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     5641 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     5701 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     5761 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     5821 cgttccgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     5881 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     5941 gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6421 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     7081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7621 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7681 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7741 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7801 gaggcccttt cgtcttcaag aattcattct tttattgagt ccagattaga aaaagaagag
     7861 aatttatgaa cggacctttt gacagatttc tttttgaaaa tactcattcc tcttcagatt
     7921 tgttttaaat gaagttttct ttctttaact tgtttgcacg cagctcagct gtgttctttc
     7981 ttaaatgagg tctggtaaag aaaaggccct accaaggtct tagctgtact tgtgacatga
     8041 tctctctatt aggttaaagt ttttttctgt tatgtgatgc tcataaaact gagttataac
     8101 aatattaata ataacttcac taattgattc tgtgagggat gagaagaggg aggaatagga
     8161 tgagaaaaac agagggaact atttgccttg tatttgacat acttagaaga taagggaaac
     8221 atgtttggaa tgtcagttcc ccaggctact ttatgtcaat gttagaaatg gaaacaatga
     8281 ttttcattgg ctacaaacag gagaggaggc tgtggtgatt ttggggtatg cgttgtgttc
     8341 attgtgattc tataagcacc ccatcttcca aagagagctc aacaatagtc aggaagcctc
     8401 gccctaaagc ccatcctccc ccccgcccca cccccatgac tttgtttcct tgtcagactt
     8461 tacctgagag gaaatgaaat taataagctg tgaaaaagta tcccatgtgg gctgctctta
     8521 agtctcccgt tgccttttcc tcctgtgttc tgcgttctag ctgtttgcga gtaggtgagg
     8581 ctggtgactc actgccttca agcagcagca ggactggtga gtcatagctt tcaggctggg
     8641 gttgtggttc actcccatgg acggtttaac ccgtggttgt ctcttagggg gtcagtggga
     8701 acatccttcg tatggtcgtg atcttcttaa atcctctccc caggaccagg caataacagt
     8761 gtcctggcaa aagctccagt actccatctt ctcaggacat cccagtccac atgactaatc
     8821 tttcccacct tctacatttg agcgtttcct gccttcacat tatagaaact attttgggaa
     8881 gtattatttt taaacatgac ttttccctta attctaccat aataagtgct gggttaagct
     8941 ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat ttgacttgtt
     9001 ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta aaatgttaat
     9061 tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca tgaatgctgc
     9121 aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa attacttaga
     9181 gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc tgagaagcca
     9241 gtttgcatct gtcaggatca atttcccatt atgccagtca tattaattac tagtcaatta
     9301 gttgattttt atttttgaca tatacatgtg aaagacccca cctgtaggtt tggcaagcta
     9361 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agaaaagttc
     9421 agatcaaggt caggaacaga tggaacag
//
