LOCUS       sequence-251735-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low level expression of E.coli G.stearothermophillus MutY chimera 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Russelburg LP, O'Shea Murray VL, Demir M, Knutsen KR, Sehgal SL, Cao
            S, David SS, Horvath MP
  TITLE     Structural Basis for Finding OG Lesions and Avoiding Undamaged G by 
            the DNA Glycosylase MutY.
  JOURNAL   ACS Chem Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639.
  PUBMED    31829624
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639."
COMMENT     Sequence Label: EcNGsC-MutY-pKK223
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     30..52
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagaa
       61 ttcggtgacc atgcaagcgt cgcaattttc agcccaggtt ctggactggt acgataaata
      121 cgggcgaaaa actctgccct ggcaaattga caagacgccc tacaaagtat ggctctcaga
      181 agtgatgttg caacaaactc aggttgcgac cgttatcccc tattttgaac gctttatggc
      241 gcgcttcccg acggtgaccg atctcgccaa tgcgccgctc gacgaagttc tccacttgtg
      301 gaccgggctt ggctattacg cccgcgcgcg caatctgcat aaagcggcac aacaagtggc
      361 gaccttacac ggcggtaaat tcccggaaac ctttgaggaa gttgcagcac tgccgggcgt
      421 cgggcgttcc accgcaggcg cgattctctc gctttctctg ggtaagcact ttccgattct
      481 cgacggtaac gtcaaacgcg tgctggcgcg ctgctatgct gtaagcggct ggcctgggaa
      541 aaaagaggtc gagaataaat tatggagttt gagcgagcag gtgacgcccg cggttggcgt
      601 ggaacggttt aatcaggcga tgatggattt gggtgcgatg atttgtacgc gctcgaaacc
      661 gaaatgttcg ctctgtccgc tacaaaacgg atgtattgcc gccgccaaca atagctgggc
      721 gctttatccg ggc
//
