LOCUS       sequence-251738-        4396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for TERT promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Bell RJ, Rube HT, Kreig A, Mancini A, Fouse SD, Nagarajan RP, Choi 
            S, Hong C, He D, Pekmezci M, Wiencke JK, Wrensch MR, Chang SM, Walsh
            KM, Myong S, Song JS, Costello JF
  TITLE     Cancer. The transcription factor GABP selectively binds and 
            activates the mutant TERT promoter in cancer.
  JOURNAL   Science. 2015 May 29;348(6238):1036-9. doi: 10.1126/science.aab0015.
            Epub 2015 May 14.
  PUBMED    25977370
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab0015"; journalName: "Science"; date: 
            "2015-05-29- 29"; volume: "348"; issue: "6238"; pages: "1036-9"
COMMENT     Sequence Label: pGL4.0-TERT WT
FEATURES             Location/Qualifiers
     source          1..4396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..81
                     /note="synthetic polyadenylation signal"
     CDS             join(186..254,255..1046)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1246..1834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1735..1754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1988..2005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2253..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2290..2309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2344..2363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2418..4070)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      4067..4076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(4324..4396,1..19)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(4356..4375)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
       61 tgaaaataaa gatattttat tgcggcctgt ccaatacctc ccgtacctta atattactta
      121 cttatccttg agagacgtac tagtaaccct gataaatgct tcaataatat tgaaaaagga
      181 cgagtatgag catccaacat tttcgtgtcg cactcattcc cttctttgcg gcattttgct
      241 tgcctgtttt tgcacacccc gaaacgctgg tgaaagtaaa agatgctgaa gatcaactgg
      301 gtgcaagagt gggctatatc gaactggatc tcaatagcgg caagatcctt gagtctttcc
      361 gccccgaaga acgattcccg atgatgagca ctttcaaagt actgctatgt ggcgcggtgt
      421 tgtcccgtat agacgccggg caagagcagc ttggtcgccg tatacactac tcacaaaacg
      481 acttggttga gtactcgccg gtcacggaaa agcatcttac ggatggcatg acggtaagag
      541 aattgtgtag tgctgccatt accatgagcg acaacaccgc ggccaactta cttctgacaa
      601 cgatcggagg ccctaaggag ctgactgcat ttcttcataa tatgggtgat catgtgaccc
      661 ggcttgaccg ctgggaacca gagttgaacg aagccatacc gaacgacgag cgtgatacca
      721 cgatgccagt agcaatggcc acaactcttc ggaaactact cactggcgaa cttcttactc
      781 tagcatcacg acagcagctc atagactgga tggaggcgga caaagtagca ggaccacttc
      841 ttcgctcggc cctccctgct ggctggttca ttgctgacaa atcgggggcc ggtgaacgcg
      901 gctctcgcgg catcattgct gcgctggggc ctgatggtaa gccctcacga atcgtagtga
      961 tctacacgac ggggagtcag gccactatgg acgaacgaaa taggcagatc gctgagatcg
     1021 gtgcctcact gatcaagcac tggtaaccac tgcagtggtt tagcatttgc ggccgctgtc
     1081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc tgccggcacc tgtcctacga
     2041 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     2101 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacggatc cttatcgatt
     2161 ttaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2221 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgctcgaag cggccggccg
     2401 ccccgactct agaattatta cacggcgatc ttgccgccct tcttggcctt aatgagaatc
     2461 tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt taggcacctc gtccacgaac
     2521 acaacaccac cgcgcagctt cttggcggtt gtaacctggc tggccacata gtccacgatc
     2581 tccttctcgg tcatggtttt accgtgttcc agcacgacga ctgcggcggg cagctcgccg
     2641 gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga tgttggggtg ttgcagcagg
     2701 atgctctcca gttcggctgg ggctacctgg tagcccttgt atttgatcag gctcttcagc
     2761 cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg cgatgtcgcc gctgtgcagc
     2821 cagccgtcct tgtcgatgag agcgtttgta gcctcggggt tgttaacgta gccgctcatg
     2881 atcatggggc cacggacgca cagctcgccg cgctggttca cacccagtgt cttaccggtg
     2941 tccaagtcca ccaccttagc ctcgaagaag ggcaccacct tgcctactgc gccaggcttg
     3001 tcgtcccctt cgggggtgat cagaatggcg ctggttgttt ctgtcaggcc gtagccctgg
     3061 cggatgcctg gtaggtggaa gcgtttggcc acggcctcac ctacctcctt gctgagcggc
     3121 gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt acttgtcgat gagagtgctc
     3181 ttagcgaaga agctaaatag tgtgggcacc agcagggcag attgaatctt atagtcttgc
     3241 aagctgcgca agaatagctc ctcctcgaag cggtacatga gcacgacccg aaagccgcag
     3301 atcaagtagc ccagcgtggt gaacatgccg aagccgtggt gaaatggcac cacgctgagg
     3361 atagcggtgt cggggatgat ctggttgccg aagatggggt cgcgggcatg actgaatcgg
     3421 acacaagcgg tgcggtgcgg tagggctacg cccttgggca atccggtact gccactactg
     3481 ttcatgatca gggcgatggt tttgtcccgg tcgaagctct cgggcacgaa gtcgtactcg
     3541 ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca tgctttggaa gccctggtag
     3601 tcggtcttgc tatccatgat gatgatcttt tgtatgatcg gtagcttctt ttgcacgttg
     3661 aggatctttt gcagcccttt cttgctcacg aatacgacgg tgggctggct gatgcccatg
     3721 ctgttcagca gctcgcgctc gttgtagatg tcgttagctg gggccacagc cacaccgatg
     3781 aacagggcac ccaacacggg catgaagaac tgcaagctat tctcgctgca caccacgatc
     3841 cgatggtttg tattcagccc atagcgcttc atagcttctg ccagccgaac gctcatctcg
     3901 aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt cggtaaaggc gatggtgccg
     3961 ggcaccaggg cgtagcgctt catggctttg tgcagctgct cgccggcggt cccgtcttcg
     4021 agtgggtaga atggcgctgg gcccttctta atgtttttgg catcttccat ggtggcttta
     4081 ccaacagtac cggattgcca agcttggccg ccgaggccag atccagcgct gcctgaaact
     4141 cgcgccgcga ggagagggcg gggccgcgga aaggaagggg aggggctggg agggcccgga
     4201 gggggctggg ccggggaccc gggaggggtc gggacggggc ggggtccgcg cggaggaggc
     4261 ggagctggaa ggtgaagggg caggactcga ggctagcgag ctcaggtacc ggccagttag
     4321 gccagagaaa tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt
     4381 tttgtttcgt tttgtt
//
